The BEL1-like family of transcription factors in potato

Sharma, Pooja; Lin, Tong; Grandellis, Carolina; Yu, Mei; Hannapel, David J.

doi:10.1093/jxb/ert432

Cited by 71 publications

(65 citation statements)

References 48 publications

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“…Concordant with Arabidopsis, there are 13 BEL1 genes in potato (Solanum tuberosum; Sharma et al, 2014). One of the StBELs, StBEL5, functions as a signal mRNA in potato, is trafficked long distance through the sieve element system, and is involved in the activation of tuberization (Banerjee et al, 2006a).…”

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confidence: 99%

Targets of the StBEL5 Transcription Factor Include the FT Ortholog StSP6A

2015

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ORCID IDs: 0000-0001-7693-8503 (P.S.); 0000-0001-8195-9202 (D.J.H.).The BEL1-like family of transcription factors is ubiquitous in plants and plays important roles in regulating development. They function in tandem with KNOTTED1 types to bind to a double TTGAC motif in the upstream sequence of target genes. StBEL5 of potato (Solanum tuberosum) functions as a mobile RNA signal that is transcribed in leaves, moves down into stolons in response to short days, and induces tuber formation. Despite their importance, however, very little is known about the targets of BEL1-like transcription factors. To better understand this network, we made use of a phloem-mobile BEL5 induction model, an ethanol-inducible system coupled with RNA sequencing analysis, and a screen for tandem TTGAC ciselements in the upstream sequence to catalog StBEL5 target genes. Induction of StBEL5 activated several genes that are also induced by StSP6A (S. tuberosum SELF-PRUNING 6A), a FLOWERING LOCUS T coregulator that functions as a signal for tuberization. Both enhancement and suppression of StBEL5 expression were also closely linked to StSP6A transcriptional activity. Site mutagenesis in tandem TTGAC motifs located in the upstream sequence of StSP6A suppressed the short dayinduced activity of its promoter in both young tubers and leaves. The expression profile of StBEL5 induced in stolons from plants grown under long-day conditions revealed almost 10,000 differentially expressed genes, including important tuber marker genes and genes involved in cell growth, transcription, floral development, and hormone metabolism. In a random screen of 200 differentially expressed targets of StBEL5, 92% contained tandem TTGAC motifs in the upstream sequence within 3 kb of the transcription start site.

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confidence: 99%

Targets of the StBEL5 Transcription Factor Include the FT Ortholog StSP6A

2015

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“…BLH form heterodimeric complexes with STM and BP. This KNOX-BLH interaction is conserved among various plant species (Mizumoto et al, 2011;Arnaud and Pautot, 2014;Sharma et al, 2014).…”

Section: Class 1 Knox Homeodomain Transcription Factorsmentioning

confidence: 98%

“…In Medicago truncatula KNOX genes present important differences compared with other characterised legume KNOXs (Di Giacomo et al, 2008;Zhou et al, 2014). (Sharma et al, 2014). BLH form heterodimeric complexes with STM and BP.…”

Section: Class 1 Knox Homeodomain Transcription Factorsmentioning

confidence: 99%

Plant development regulation: Overview and perspectives

Yruela

2015

Journal of Plant Physiology

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Plant development, as occur in other eukaryotes, is conducted through a complex network of hormones, transcription factors, enzymes and micro RNAs, among other cellular components.They control developmental processes such as embryo, apical root and shoot meristem, leaf, flower, or seed formation, among others. The research in these topics has been very active in last decades. Recently, an explosion of new data concerning regulation mechanisms as well as the response of these processes to environmental changes has emerged. Initially, most of investigations were carried out in the model eudicot Arabidopsis but currently data from other plant species are available in the literature, although they are still limited. The aim of this review is focused on the current knowledge of main molecular actors involved in plant development regulation in diverse plant species. A special attention will be given to the major families of genes and proteins participating in these mechanisms. The information on the regulatory pathways where they participate will be briefly cited. Additionally, he importance of certain structural features of such proteins that confer ductility and flexibility to these mechanisms will also be reported and discussed.

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“…These proteins were indeed shown to interact each other and bind the GA2oxidase1, YUCCA and IPT promoters to activate expression of these genes, hence it was proposed that they induce tuberization by regulating hormonal levels in the stolons [42,43]. However, direct evidence showing that these mobile RNAs are required for tuberization could not be obtained due to redundant function of members of these gene families [45,46].…”

Section: Other Mobile Signals Controlling Potato Tuberizationmentioning

confidence: 99%