The behavior of inorganic phosphate in the blood and urine of normal and diabetic subjects during carbohydrate metabolism

Abstract

have recently called atteation to the fall of the concentration of the inorganic phosphate in the blood of human subjects and experimental animals following the use of insulin. In the course of various studies on diabetes, particularly in connection with the studies on the respiratory exchange of normal and diabetic subjects following the ingestion of glucose, glycerol, calcium hexose phosphate and calcium glycerophosphate' and the respiratory exchange following the administration of insulin and epinephrin4 data were accumulated on the changes of the inorganic phosphate in the blood and urine under the above enumerated experimental conditions. It was found that in normal human subjects the ingestion of 50-100 gm. of glucose and the intravenous injection of 3.5 units of insulin, also the subcutaneous injection of 0.5 cc. of 1 :lo00 solution of epinephrin caused a marked fall in the inorganic phosphate of the blood, and a simultaneous fall of the rate of excretion of phosphate in the urine. With the injection of epinephrin, however, in normal subjects the faltl of the blood phosphate was frequently accompanied by a rise in the rate of urinary excretion of phosphate. The excretion of phosphate was most singularly depressed by the IHarrop, G. A., and Benedict, E. M., Paw. SOC.

Propranolol Block of Adrenaline-Induced Hypophosphataemia in Man

“…Experiments with 32P have shown that it is due to a more rapid removal of phosphate from the plasma (Hevesy & Dal Santo, 1954;Dury, 1955); since a renal mechanism seems out of the question (Perlzweig et al, 1923;Allan, Dickson & Markowitz, 1924), this would seem to be a tissue-wards movement, as suggested by Cori &Cori (1931) andHevesy &Dal Santo (1954).…”

Effect of Fasting and Insulin on the Glucagon-lnduced Orthophosphate Incorporation to the Isolated Perfused Rat Liver

“…This phenomenon may explain, in part, the well-known hypophosphatemic effect exhibited by these glycogenolytic factors (2)(3)(4)(5)(6). The functional meaning of the increased orthophosphate incorporation to the liver has not been clarified yet.…”