The behavior of HeLa-S3 cells under the influence of supranormal temperatures

An in-vitro study of the effects of temperature on breast cancer cells: Experiments and models

“…Heat causes a cellular stress which triggers a cascade of molecular events. Studies have shown that heat affects nuclear function through the inhibition of RNA [12][13][14][15], DNA [16,17], and protein synthesis [16][17][18]. In addition, hyperthermia causes delay or arrest in cell cycle progression [19]; chiefly through mitotic arrest [20][21][22][23] and inhibiting S phase entry from G 2 [24,25].…”

“…Within the last decade, researchers have developed multiple delivery modalities for hyperthermia and tested them in both in-vitro and in-vivo conditions as previously described [12,17,38,39]. The most common is the use of ferromagnetic fluids in combination with an oscillating magnetic field [40][41][42].…”

Tissue-Type-Specific Heat-Shock Response and Immunolocalization of Class I Low-Molecular-Weight Heat-Shock Proteins in Soybean

“…These observations are strongly correlated with the inhibition of rRNA processing and ribosome assembly that is rapidly initiated after the beginning of HS (Arrigo et al, 1980;Neumann et al, 1984;Hadjiolov, 1985;Bell et al, 1988). Structural changes in the cytoplasm, mitochondrion, chloroplast, ER, and dictyosome have also been reported after HS (Heine et al, 1971;Skogqvist, 1974;Schnepf and Schmitt, 1981;Craig, 1985;Welch and Suhan, 1985).…”

“…The most significant change in the ultrastructure of a11 organisms under HS occurs within the nucleolus, where the granular components are broken down, the fibrillar components became more prominent, and the degree of vacuolization is increased (Simard and Bernhard, 1967;Heine et al, 1971;Fransolet et al, 1979;Neumann et al, 1984;Chen et al, 1988;. These observations are strongly correlated with the inhibition of rRNA processing and ribosome assembly that is rapidly initiated after the beginning of HS (Arrigo et al, 1980;Neumann et al, 1984;Hadjiolov, 1985;Bell et al, 1988).…”

Ultrastructural detection of nucleic acids within heat shock-induced perichromatin granules of HeLa cells by cytochemical and immunocytological methods

“…The number of PGs is estimated at between 500 and 2000 by nucleus of mammalian cells (Watson, 1962;Monneron and Bernhard, 1969) and varies depending on the physiological conditions. For examples, an increased number of PGs has been observed when the cells are subjected to hyper-or hypo-thermal shocks (Heine et al, 1971;Puvion et al, 1977;Cervera and Montero, 1980;Mähl et al, 1989) and when the cells come into apoptosis (Miller et al, 2002) or hibernation (Biggiogera and Pellicciari, 2000). Cycloheximide, an inhibitor of protein synthesis (Daskal et al, 1975;Lafarga et al, 1993), a-amanitin, an inhibitor of polymerase II and III (Derenzini and Moyne, 1978), 5-6-dichloro-1-bribofuranosyl benzimidazole, an inhibitor of mRNAs synthesis , all cause an increase in the number of PGs in treated cells.…”