The band forming rhythm of <i>Neurospora</i> mutants

Berliner, Martha D.; Neurath, Peter W.

doi:10.1002/jcp.1030650206

Cited by 21 publications

(10 citation statements)

References 8 publications

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“…which a t least implies light-dark cycle control and nutritional independence which these and other Neurospora strains do not have (1). This report gives evidence for the specific chemical control, by sugars, of the rhythm in Neurospora.…”

mentioning

confidence: 62%

Control of Rhythmic Growth of a Neurospora "Clock" Mutant by Sugars

Berliner¹,

Neurath²

1966

Can. J. Microbiol.

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confidence: 62%

Control of Rhythmic Growth of a Neurospora "Clock" Mutant by Sugars

Berliner¹,

Neurath²

1966

Can. J. Microbiol.

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“…The rhythm in clock is described as a hyphal branching rhythm, and published reports disagree as to whether it may (Bianchi, 1964) or may not (Neurath and Berliner, 1964;Sussman et al, 1964) be accompanied by conidiation. This rhythm also may (Sussman et al, 1964) or may not (Bianchi, 1964;Berliner and Neurath, 1965) be damped out in constant light, and cannot be entrained to LD cycles (Neurath and Berliner, 1964). The growth rhythm in clock is temperature compensated above 20°C (Neurath and Berliner, 1964;Sussman et al, 1965).…”

Section: Discussionmentioning

confidence: 96%

Effects of Choline Depletion on the Circadian Rhythm in Neurospora crassa

Lakin-Thomas¹

1996

Biological Rhythm Research

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One approach to identifying components of the circadian oscillator is to screen for clock defects in mutants with known biochemical lesions. The chol-1 mutant of Neurospora crassa is defective in the first methylation step of phosphatidylcholine synthesis, the conversion of phosphatidylethanolamine to phosphatidylmonomethylethanolamine, and requires choline for normal growth. Choline depletion of this mutant inhibits growth and lengthens the period of the rhythm of conidiation. On high levels of choline (above 20 µM), the growth rate and the period of the rhythm are normal. Below about 10 µM choline, the growth rate and period length depend on the choline concentration, and the period is about 58 h on minimal medium without choline. Choline depletion decreases period stability, and replicate cultures do not remain in phase due to variability in period within each culture. At intermediate levels of choline (around 10 µM) cultures are often arrhythmic. The choline requirement for growth can be met by the phosphatidylcholine precursors monomethylethanolamine and dimethylethanolamine, and these supplements also restore a normal period.Choline depletion of the chol-1 strain exaggerates the rhythm in growth rate previously reported in a chol + strain. Growth rate during formation of a conidial band (measured as forward advance of the mycelial front) is less than half of the maximum rate during non-conidiating interband formation. Choline-depleted cultures can be entrained to light/dark (LD) cycles with periods near to their freerunning periods. Cultures on 10 µM choline (with a free-running period of about 25 h) can be entrained to a 24 h (12:12) LD cycle, but not to a 36 h (18:18) or 48 h (24:24) LD cycle. Cultures on 0.5 µM choline (free-running period of about 52 h) or minimal medium (free-running period of about 58 h) can be entrained to 18:18 and 24:24 LD cycles, but not a 12:12 cycle. The phase relationship of the conidiation rhythm to the zeitgeber for low-choline cultures in LD 24:24 is similar to high choline cultures in LD 12:12. Continuous light abolishes rhythmicity in choline-depleted cultures.These results may indicate a role for membrane phospholipids, and the metabolites of phosphatidylcholine in particular, in the control of the period of the circadian oscillator in Neurospora.

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“…In general, perturbing the metabolism of an organism has little effect on its circadian rhythmicity (9), but changes in rhythm expression and period have been demonstrated in certain strains of Neurospora and other fungi by supplementing the growth medium with appropriate sugars and amino acids (3,5,10).…”

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Effects of Medium Composition and Carbon Dioxide on Circadian Conidiation in Neurospora

1972

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Efforts to significantly perturb the timing mechanism, and thus the period, of the rhythm responsible for circadian conidiation in bd, a strain of Neurospora crassa, by altering the medium composition have been unsuccessful. Various salt solutions, sugars, and amino acids do, however, have pronounced effects on growth and conidiation, and thus on the expression and persistence of rhythmicity.Aeration August, 1970. perturb the postulated timing mechanism is to vary the growth medium. In general, perturbing the metabolism of an organism has little effect on its circadian rhythmicity (9), but changes in rhythm expression and period have been demonstrated in certain strains of Neurospora and other fungi by supplementing the growth medium with appropriate sugars and amino acids (3, 5, 10).The work reported here was initiated to determine if varying the growth medium would provide useful information about the mechanisms responsible for the bd rhythmicity. The experiments provide a better understanding of the band-gene role in Neurospora rhythmicity, and suggest that aeration is an important variable in the expression of Neurospora rhythmicity. The effects of aeration provide new insight into the occurrence of rhythmicity in Neurospora and the role of carbon dioxide in regulating conidiation of Neurospora cultures. MATERIALS AND METHODSStrains. A single-mutant strain, band (bd), which exhibits the timex phenotype on our standard glucose-arginine medium (16) was used throughout. This strain, MLS 41-4 (bd, a), was isolated after three backcrosses of the original bd (E7a) into the St. Lawrence wild-type, 74-OR 23-1A. The sources of the other wild-type and exotic strains used have been reported, as have the methods of stock maintenance and preservation (18).Media. The media contained Vogel's salts (22), 1.5% Difco Bacto-agar (unless otherwise stated), and the supplements listed. All percentage designations for media are weight per volume. The media were prepared, autoclaved, and dispensed (8 ml/tube) into 55-cm Pyrex growth-tubes stoppered with cotton. Heat-labile amino acids were filter sterilized and added to warm medium (45-50 C) before dispensing.Growth Conditions. Growth-tube cultures were inoculated, incubated 18 to 20 hr at room temperature in the light, and then placed in continuous darkness at 25 C. Scoring of rhythmicity and period calculations have been described previously (16, 18

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The band forming rhythm of Neurospora mutants

Cited by 21 publications

References 8 publications

Control of Rhythmic Growth of a Neurospora "Clock" Mutant by Sugars

Control of Rhythmic Growth of a Neurospora "Clock" Mutant by Sugars

Effects of Choline Depletion on the Circadian Rhythm in Neurospora crassa

Effects of Medium Composition and Carbon Dioxide on Circadian Conidiation in Neurospora

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