The BAH domain facilitates the ability of human Orc1 protein to activate replication origins in vivo

Noguchi, Kohji; Vassilev, Alex; Ghosh, Sourish; Yates, John L.; DePamphilis, Melvin L.

doi:10.1038/sj.emboj.7601396

Cited by 81 publications

(96 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine whether or not HsOrc6 in its native environment is bound to a nuclear transport protein, FH-Orc6 was purified by double affinity chromatography from HeLa cells that expressed it constitutively. Previous applications of this strategy demonstrated that constitutively expressed FH-Orc1 and FHOrc2 proteins modeled their endogenous counterparts (8,9). They are assembled into ORCs in the nuclei of G 1 -phase cells where they preferentially bind to DNA replication origins and stimulate DNA replication.…”

Section: Orc6mentioning

confidence: 99%

“…Mammalian ORC(2-5) can assemble spontaneously in vitro from individual ORC subunits under a variety of conditions (7)(8)(9)(10)(11)(12)(13)(14). Assembly begins with formation of an ORC(2, 3, 5) complex to which Orc4 binds in a manner that requires binding of ATP to both the Orc4 and Orc5 subunits (11,12) (Fig.…”

Section: Nuclear Localization Of Orc6-humanmentioning

confidence: 99%

“…ORC isolated from frog eggs lacks the Orc6 subunit, which is not essential for DNA replication (6). ORC isolated from human cells also lacks the Orc6 subunit (7)(8)(9)(10), and only the human ORC (1)(2)(3)(4)(5) complex is required to initiate DNA replication in an ORC-depleted frog egg extract (9,10). Human ORC (2)(3)(4)(5) binds to DNA and initiates DNA replication only when associated with Orc1 (9,10,26).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Assembly of the Human Origin Recognition Complex Occurs through Independent Nuclear Localization of Its Components

Ghosh

Vassilev

Zhang

et al. 2011

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Initiation of eukaryotic genome duplication begins when a six-subunit origin recognition complex (ORC) binds to DNA. However, the mechanism by which this occurs in vivo and the roles played by individual subunits appear to differ significantly among organisms. Previous studies identified a soluble human ORC(2-5) complex in the nucleus, an ORC(1-5) complex bound to chromatin, and an Orc6 protein that binds weakly, if at all, to other ORC subunits. Here we show that stable ORC(1-6) complexes also can be purified from human cell extracts and that Orc6 and Orc1 each contain a single nuclear localization signal that is essential for nuclear localization but not for ORC assembly. The Orc6 nuclear localization signal, which is essential for Orc6 function, is facilitated by phosphorylation at its cyclin-dependent kinase consensus site and by association with Kpna6/1, nuclear transport proteins that did not co-purify with other ORC subunits. These and other results support a model in which Orc6, Orc1, and ORC(2-5) are transported independently to the nucleus where they can either assemble into ORC(1-6) or function individually.

show abstract

Section: Orc6mentioning

confidence: 99%

Section: Nuclear Localization Of Orc6-humanmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Assembly of the Human Origin Recognition Complex Occurs through Independent Nuclear Localization of Its Components

Ghosh

Vassilev

Zhang

et al. 2011

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Among the ORC subunits in eukarya, only Orc1 has a BAH domain that mediates protein -protein interactions. The BAH domain in human Orc1 facilitates the ability of Orc1 to activate replication origins in vivo (Noguchi et al 2006), and links H4K20me2 to DNA replication licensing and the Meier-Gorlin syndrome, primordial dwarfism that is related directly to mutations in prereplication proteins (Bicknell et al 2011;Kuo et al 2012).…”

Section: Dna Replication Proteins the Prereplication Complexmentioning

confidence: 99%

Regulating DNA Replication in Plants

Sánchez

Costas

Sequeira-Mendes

et al. 2012

Cold Spring Harbor Perspectives in Biology

View full text Add to dashboard Cite

Chromosomal DNA replication in plants has requirements and constraints similar to those in other eukaryotes. However, some aspects are plant-specific. Studies of DNA replication control in plants, which have unique developmental strategies, can offer unparalleled opportunities of comparing regulatory processes with yeast and, particularly, metazoa to identify common trends and basic rules. In addition to the comparative molecular and biochemical studies, genomic studies in plants that started with Arabidopsis thaliana in the year 2000 have now expanded to several dozens of species. This, together with the applicability of genomic approaches and the availability of a large collection of mutants, underscores the enormous potential to study DNA replication control in a whole developing organism. Recent advances in this field with particular focus on the DNA replication proteins, the nature of replication origins and their epigenetic landscape, and the control of endoreplication will be reviewed.

show abstract

“…The BAH domain of ORC1 is important for the activation of EBNA1-dependent origins of DNA synthesis. Deletion of the entire domain (AA1-169), as well as a single point mutation (E111K), reduced the association of ORC1 with DS to background as measured by Chromatin ImmunoPrecipitation (ChIP) (Noguchi, Vassilev et al 2006). These same modifications also reduced the short-term replication of oriP plasmids to background levels, and the overexpression of the BAH domain alone (AA 1-315) functioned dominant-negatively to inhibit oriP replication (ibid).…”

mentioning

confidence: 99%

The plasmid replicon of Epstein–Barr virus: Mechanistic insights into efficient, licensed, extrachromosomal replication in human cells

Lindner

Sugden

2007

Plasmid

103

View full text Add to dashboard Cite

The genome of Epstein-Barr Virus (EBV) and plasmid derivatives of it are among the most efficient extrachromosomal replicons in mammalian cells. The latent origin of plasmid replication (oriP), when supplied with the viral Epstein-Barr Nuclear Antigen 1 (EBNA1) in trans, provides efficient duplication, partitioning and maintenance of plasmids bearing it. In this review, we detail what is known about the viral cis and trans elements required for plasmid replication. In addition, we describe how the cellular factors that EBV usurps are used to complement the functions of the viral constituents. Finally, we propose a model for the sequential assembly of an EBNA1-dependent origin of DNA synthesis into a pre-Replicative Complex (pre-RC), which functions by making use only of cellular enzymatic activities to carry out the replication of the viral plasmid. KeywordsoriP; EBNA1; EBV; origin; DS; Rep* General BackgroundEpstein-Barr Virus (EBV), a member of the herpesvirus family, is a surprisingly successful parasite, as are other human members of this family of viruses. It establishes a persistent, lifelong infection in greater than 90% of the world's population (Fields, Knipe et al. 2006). Primary infection by EBV causes infectious mononucleosis in some, usually adolescent people (Evans, Niederman et al. 1968). The latent cycle of the virus that follows infection is usually asymptomatic in the host, however in a small fraction of infected people, EBV contributes to several cancers including Burkitt's lymphoma, some T-cell lymphomas, Hodgkin's disease, post-transplant lymphoproliferative disease, nasopharyngeal carcinoma, and gastric carcinoma. EBV was the first human virus to be classified as a tumor virus (reviewed in Chapter 75 of (Fields, Knipe et al. 2006)) and has been studied because of its association with these diseases. EBV, in addition, has also served as a model to study DNA replication in human cells because of it's ability both to maintain its genome as an extrachromosomal replicon in infected B-cells, and to appropriate the cellular DNA replication machinery needed for its licensed replication.* To whom correspondence should be addressed: 1400 University Ave, Madison, WI 53706, Phone: 608.262.6697, Fax: 608.262.2824, Email: sugden@oncology.wisc.edu. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Cis Elements of EBV that Contribute to DNA ReplicationThe genome of EBV is approximately 165kbp (Bloss and Sugden 1994) and resides as a nucleosome-coated nuclear plasmid in infected cells (Wensing and Farrell 2000), (Shaw, Levinger et al. 1979)...

show abstract

The BAH domain facilitates the ability of human Orc1 protein to activate replication origins in vivo

Cited by 81 publications

References 57 publications

Assembly of the Human Origin Recognition Complex Occurs through Independent Nuclear Localization of Its Components

Assembly of the Human Origin Recognition Complex Occurs through Independent Nuclear Localization of Its Components

Regulating DNA Replication in Plants

The plasmid replicon of Epstein–Barr virus: Mechanistic insights into efficient, licensed, extrachromosomal replication in human cells

Contact Info

Product

Resources

About