The BAH (bromo‐adjacent homology) domain: a link between DNA methylation, replication and transcriptional regulation

Callebaut, Isabelle; Courvalin, Jean-Claude; Mornon, Jean‐Paul

doi:10.1016/s0014-5793(99)00132-5

Cited by 190 publications

(178 citation statements)

References 46 publications

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“…Misexpression of wge induces eye-to-wing TD by altering both wg and vg expression. wge displays trxG-like characteristics and encodes a chromatin-associated protein that includes a bromo-adjacent homology (BAH) domain implicated in epigenetic regulation of gene expression (Callebaut, Courvalin, & Mornon, 1999). Taken together, these studies indicate that developmental potency and the reprogramming of cellular fate are closely allied with the maintenance of both chromatin organization and fate-specific transcriptional programs.…”

Section: Developmental Potency and Chromatin Reorganizationmentioning

confidence: 97%

Transdetermination: Drosophila imaginal disc cells exhibit stem cell-like potency

McClure

Schubiger

2007

The International Journal of Biochemistry & Cell Biology

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Drosophila imaginal discs, the primordia of the adult fly appendages, are an excellent system for studying developmental plasticity. Cells in the imaginal discs are determined for their disc-specific fate (wingness, legness) during embryogenesis. Disc cells maintain their determination during larval development, a time of extensive growth and proliferation. Only when prompted to regenerate do disc cells exhibit lability in their determined identity. Regeneration in the disc is mediated by a localized region of cell division, known as the regeneration blastema. Most regenerating disc cells strictly adhere to their disc-specific identity; some cells however, switch fate in a phenomenon known as transdetermination. Similar regeneration and transdetermination events can be induced in situ by misexpression of the signaling molecule wingless. Recent studies indicate that the plasticity of disc cells during regeneration is associated with high morphogen activity and the reorganization of chromatin structure. Here we provide both a historical perspective of imaginal disc transdetermination, as well as discuss recent findings on how imaginal disc cells acquire developmental plasticity and multipotency. We also highlight how an understanding of imaginal disc transdetermination can enhance an understanding of developmental potency exhibited by stem cells.

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Section: Developmental Potency and Chromatin Reorganizationmentioning

confidence: 97%

Transdetermination: Drosophila imaginal disc cells exhibit stem cell-like potency

McClure

Schubiger

2007

The International Journal of Biochemistry & Cell Biology

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“…It also contains the Polybromo protein that is not present in the BAF complex. Polybromo, also called BAF180, contains six successive Bromodomains and shares some homology with three subunits of the yeast RSC complex, Rsc1, Rsc2 and Rsc4, both within and (Papoulas et al, 1998), 2 (Kwon et al, 1994;Nie et al, 2000), 3 (Kwon et al, 1994;Xue et al, 2000), 4 (Underhill et al, 2000), 5 (Du et al, 1998;Tsuchiya et al, 1998), 6 (Tamkun et al, 1992), 7 (Chiba et al, 1994;Khavari et al, 1993;Muchardt and Yaniv, 1993), 8 (Cao et al, 1997), 9 (Dingwall et al, 1995), 10 (Kalpana et al, 1994;Muchardt et al, 1995), 11 (Kal et al, 2000), 12 , 13 (Zhao et al, 1998), 14 (Cairns et al, 1999), 15 ¯anking the Bromodomains (Cairns et al, 1999;Callebaut et al, 1999). The presence of Polybromo in the PBAF complex suggests that PBAF may in fact correspond to the yeast RSC complex.…”

Section: Swi/snf Complexes In Higher Eucaryotesmentioning

confidence: 99%

When the SWI/SNF complex remodels … the cell cycle

2001

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Mammalian cells contain several chromatin-remodeling complexes associated with the Brm and Brg1 helicaselike proteins. These complexes likely represent the functional homologs of the SWI/SNF and RSC complexes found in Saccharomyces cerevisiae. The mammalian chromatin-remodeling complexes are involved in both activation and repression of a variety of genes. Several lines of evidence also indicate that they play a speci®c role in the regulation of cell growth. Brm is down-regulated by ras signaling and its forced reexpression suppresses transformation by this oncogene. Besides, the Brg1 gene is silenced or mutated in several tumors cell lines and a Brg1-associated complex was recently found to co-purify with BRCA1, involved in breast and ovarian cancers. Finally, the gene encoding SNF5/Ini1, a subunit common to all mammalian SWI/ SNF complexes, is inactivated in rhabdoid sarcomas, a very aggressive form of pediatric cancer. The current review will address observations made upon inactivation of Brm, Brg1 and SNF5/Ini1 by homologous recombination in the mouse, as well as the possible implication of these factors in the regulation of the Retinoblastoma pRb-mediated repression of the transcription factor E2F. Oncogene (2001) 20, 3067 ± 3075.

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“…BAHD1 is so named because it contains a C-terminal BAH domain, which is found in several chromatin-binding proteins involved in transcriptional repression (9), including the yeast Sir3 protein (10). We investigated whether BAHD1 could be involved in heterochromatin formation using a combination of approaches, including two-hybrid screen, knockdown, and gain-offunction experiments.…”

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confidence: 99%

Human BAHD1 promotes heterochromatic gene silencing

Bierne

Tham

Batsché

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

125

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Gene silencing via heterochromatin formation plays a major role in cell differentiation and maintenance of homeostasis. Here we report the identification and characterization of a novel heterochromatinization factor in vertebrates, bromo adjacent homology domaincontaining protein 1 (BAHD1). This nuclear protein interacts with HP1, MBD1, HDAC5, and several transcription factors. Through electron and immunofluorescence microscopy studies, we show that BAHD1 overexpression directs HP1 to specific nuclear sites and promotes the formation of large heterochromatic domains, which lack acetyl histone H4 and are enriched in H3 trimethylated at lysine 27 (H3K27me3). Furthermore, ectopically expressed BAHD1 colocalizes with the heterochromatic inactive X chromosome (Xi). The BAH domain is required for BAHD1 colocalization with H3K27me3, but not with the Xi chromosome. As highlighted by whole genome microarray analysis of BAHD1 knockdown cells, BAHD1 represses several proliferation and survival genes, in particular the insulin-like growth factor II gene (IGF2). When overexpressed, BAHD1 specifically binds the CpG-rich P3 promoter of IGF2, which increases MBD1 and HDAC5 targeting at this locus. This region contains DNA-binding sequences for the transcription factor SP1, with which BAHD1 coimmunoprecipitates. Collectively, these findings provide evidence that BAHD1 acts as a silencer by recruiting at specific promoters a set of proteins that coordinate heterochromatin assembly.BAH domain ͉ epigenetics ͉ heterochromatin ͉ HP1 ͉ polycomb G ene repression in eukaryotes in response to developmental or environmental signals is a complex multistep process requiring the concerted action of many cellular factors, including DNA-binding transcription factors and cofactors. It also involves chromatin components and chromatin-binding/-modifying proteins, which are implicated in the establishment and maintenance of a condensed chromatin state, also referred to as facultative heterochromatin (1). Deregulation of these chromatin regulatory factors and aberrant chromatin modifications play important roles in various human diseases, including cancers, developmental abnormalities, and neurologic disorders (2-6). Moreover, several recent reports have linked histone modifications and infectious diseases (7). During a screen for human proteins that interact with microbial factors and that might play a role in bacterial pathogenicity, we identified a putative chromatin-associated protein, bromo adjacent homology domaincontaining protein 1 (BAHD1), which remains uncharacterized. One study correlated repression of the BAHD1 gene to poor prognosis in lung cancer (8), raising the possibility that BAHD1 might act as a tumor suppressor. This connection between BAHD1 and pathological processes prompted us to characterize the function of this protein.BAHD1 is so named because it contains a C-terminal BAH domain, which is found in several chromatin-binding proteins involved in transcriptional repression (9), including the yeast Sir3 protein (10). We invest...

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The BAH (bromo‐adjacent homology) domain: a link between DNA methylation, replication and transcriptional regulation

Cited by 190 publications

References 46 publications

Transdetermination: Drosophila imaginal disc cells exhibit stem cell-like potency

Transdetermination: Drosophila imaginal disc cells exhibit stem cell-like potency

When the SWI/SNF complex remodels … the cell cycle

Human BAHD1 promotes heterochromatic gene silencing

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