The bactericidal agent triclosan modulates thyroid hormone-associated gene expression and disrupts postembryonic anuran development

Veldhoen, Nik; Skirrow, Rachel C.; Osachoff, Heather L.; Wigmore, Heidi; Clapson, David J.; Gunderson, Mark P.; Aggelen, Graham van; Helbing, Caren C.

doi:10.1016/j.aquatox.2006.08.010

Cited by 332 publications

(187 citation statements)

References 46 publications

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“…Some of these AWIs are unaltered or incompletely removed in wastewater-treatment plants (WWTPs), and subsequently have been identified in the environment, especially in surface waters receiving wastewater effluent (9)(10)(11)(12). Various studies have raised concerns about the potential impacts of the environmental presence of AWIs on humans and wildlife, which includes reproductive impairment and antibiotic resistance among pathogenic bacteria (13)(14)(15)(16)(17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation of Pharmaceuticals and Other Anthropogenic Waste Indicators in Earthworms from Agricultural Soil Amended With Biosolid or Swine Manure

Kinney

Furlong

Kolpin

et al. 2008

Environ. Sci. Technol.

319

215

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Analysis of earthworms offers potential for assessing the transfer of organic anthropogenic waste indicators (AWIs) derived from land-applied biosolid or manure to biota. Earthworms and soil samples were collected from three Midwest agricultural fields to measure the presence and potential for transfer of 77 AWIs from land-applied biosolids and livestock manure to earthworms. The sites consisted of a soybean field with no amendments of human or livestock waste (Site 1), a soybean field amended with biosolids from a municipal wastewater treatment plant (Site 2), and a cornfield amended with swine manure (Site 3). The biosolid applied to Site 2 contained a diverse composition of 28 AWIs, reflecting the presence of human-use compounds. The swine manure contained 12 AWIs, and was dominated by biogenic sterols. Soil and earthworm samples were collected in the spring (about 30 days after soil amendment) and fall (140−155 days after soil amendment) at all field sites. Soils from Site 1 contained 21 AWIs and soil from Sites 2 and 3 contained 19 AWIs. The AWI profiles at Sites 2 and 3 generally reflected the relative composition of AWIs present in waste material applied. There were 20 AWIs detected in earthworms from Site 1 (three compounds exceeding concentrations of 1000 µg/kg), 25 AWIs in earthworms from Site 2 (seven compounds exceeding concentrations of 1000 µg/kg), and 21 AWIs in earthworms from Site 3 (five compounds exceeding concentrations of 1000 µg/kg). A number of compounds that were present in the earthworm tissue were at concentrations less than reporting levels in the corresponding soil samples. The AWIs detected in earthworm tissue from the three field sites included pharmaceuticals, synthetic fragrances, detergent metabolites, polycyclic aromatic hydrocarbons (PAHs), biogenic sterols, disinfectants, and pesticides, reflecting a wide range of physicochemical properties. For those contaminants detected in earthworm tissue and soil, bioaccumulation factors (BAF) ranged from 0.05 (galaxolide) to 27 (triclosan). This study documents that when AWIs are present in source materials that are land applied, such as biosolids and swine manure, AWIs can be transferred to earthworms.

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Section: Introductionmentioning

confidence: 99%

Bioaccumulation of Pharmaceuticals and Other Anthropogenic Waste Indicators in Earthworms from Agricultural Soil Amended With Biosolid or Swine Manure

Kinney

Furlong

Kolpin

et al. 2008

Environ. Sci. Technol.

319

215

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“…In addition, some native amphibian species, especially some species of the Rana genus, such as Rana catesbeiana (Veldhoen et al, 2006), Rana pipiens (Croteau et al, 2009), Rana temporaria (Mortensen et al, 2006) and Rana rugosa (Oka et al, 2009), have also been used as experimental species for assaying the endocrine-disrupting effects of chemicals. Rana nigromaculata (also called as Pelophylax nigromaculatus) is a species of true frog distributed widely in East Asia.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and mRNA expression of ribosomal protein L8 in Rana nigromaculata during development and under exposure to hormones

Lou

Cao

et al. 2014

Journal of Environmental Sciences

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Like Xenopus laevis, some species of the Rana genus are also used to study endocrine disrupting chemicals (EDCs). Although ribosomal protein L8 (rpl8) is the most-used reference gene for analyzing gene expression by quantitative reverse transcription polymerase chain reaction in Rana, its suitability as the reference gene has never been validated in any species of the Rana genus. We characterized rpl8 cDNA in Rana nigromaculata, a promising native species in East Asia for assaying endocrine disrupting effects. We found that the rpl8 cDNA consisted of 919 bp and encoded 257 amino acids, exhibiting high identities of amino acid sequence with known rpl8 in other Rana species. Then, we examined the stability of mRNA expression during development. Compared with elongation factor 1 alpha 1, another common housekeeping gene, neither stage-specific nor tissue-specific expression of the rpl8 gene was found in all tissues examined (brain, liver, intestine, tail, testis and ovary) during R. nigromaculata development. Finally, we investigated rpl8 expression under exposure to hormones. No change in rpl8 mRNA expression was found under exposure to thyroid hormone (T4) and estrogen (estradiol), whereas expression of the corresponding biomarker genes was induced.Our results show that rpl8 is an appropriate reference gene for analyzing gene expression by quantitative reverse transcription polymerase chain reaction for assaying EDCs using R. nigromaculata, and might also provide support for using rpl8 as a reference gene in other Rana species due to the high conservation of rpl8 among the Rana genus.

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“…The former is believed to play key roles in establishing tissue competence to respond to THs and in cell proliferation, whereas the latter functions in cell differentiation and apoptosis (Denver et al, 2009;Furlow and Neff, 2006). Expression of TRs (in particular TRβ) has been used as a molecular biomarker for assaying TH signaling disrupting actions in X. laevis because of their response to TH Veldhoen et al, 2006a). To validate R. nigromaculata as a native model species for assaying TH signaling disrupting effects in future study, in this study, we investigated the responsiveness of TR expression to an exogenous TR agonist (T3) and antagonist (AM) in R. nigromaculata tadpoles.…”

Section: Responsiveness Of Tr Expression To Exogenous Tr Agonist and mentioning

confidence: 99%

Molecular characterization and developmental expression patterns of thyroid hormone receptors (TRs) and their responsiveness to TR agonist and antagonist in Rana nigromaculata

Lou

Zhang

Ren

et al. 2014

Journal of Environmental Sciences

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Considering some advantages of Rana nigromaculata as an experimental species, we propose that this species, like Xenopus laevis, could be used to assay thyroid hormone (TH) signaling disrupting actions. To validate the utilizability of R. nigromaculata, we investigated the responsiveness of R. nigromaculata to a TH receptor (TR) agonist (T3) and antagonist (amiodarone) by analyzing expression, based on characterizing TR cDNA and developmental expression patterns. With high levels of identity with the corresponding genes in X. laevis, both TRα and TRβ in R. nigromaculata exhibited roughly similar developmental expression patterns to those of X. laevis, in spite of some species-specific differences. Both TRα and TRβ expression had greater changes in the liver and intestine than in the tail and brain during metamorphosis. T3 exposure for 2 days induced more dramatic increases of TRβ expression in stage 27 than in stage 34 tadpoles but not in stage 42 tadpoles, showing that the responsiveness of R. nigromaculata to TH decreased with development and disappeared at the onset of metamorphic climax.Corresponding to greater changes of TRβ expression in the liver and intestine than in the tail and brain during metamorphosis, the liver and intestine had higher responsiveness to exogenous T3 than the tail and brain. Amiodarone inhibited T3-induced TRβ expression. Our results show that R. nigromaculata can be used as a model species for assaying TH signaling disrupting actions by analyzing TRβ expression, and intestine tissues at stage 27 are ideal test materials due to high responsiveness and easy accessibility.

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The bactericidal agent triclosan modulates thyroid hormone-associated gene expression and disrupts postembryonic anuran development

Cited by 332 publications

References 46 publications

Bioaccumulation of Pharmaceuticals and Other Anthropogenic Waste Indicators in Earthworms from Agricultural Soil Amended With Biosolid or Swine Manure

Bioaccumulation of Pharmaceuticals and Other Anthropogenic Waste Indicators in Earthworms from Agricultural Soil Amended With Biosolid or Swine Manure

Molecular characterization and mRNA expression of ribosomal protein L8 in Rana nigromaculata during development and under exposure to hormones

Molecular characterization and developmental expression patterns of thyroid hormone receptors (TRs) and their responsiveness to TR agonist and antagonist in Rana nigromaculata

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