The bacterial tryptophan reverse mutation assay with Escherichia coli WP2

Mortelmans, Kristien; Riccio, Edward S.

doi:10.1016/s0027-5107(00)00076-2

Cited by 112 publications

(86 citation statements)

References 28 publications

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“…38 It should also be noted that our system is compatible with the genome-borne Trp system and the F′-borne LacZ reversion system. 15,16,42 This compatibility provides unique opportunities to independently measure the mutational events in genomes and plasmids, which is especially useful when searching for genome-specific or plasmid-specific mutagenic factors.…”

Section: Discussionmentioning

confidence: 99%

A System for the Rapid Determination of the Mutation Spectrum in Escherichia coli

et al. 2012

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The first step toward elucidating the mutagenic effects of chemicals and pathways is to determine the specificity of the mutations generated spontaneously or in response to treatment with mutagens. We constructed a set of plasmid-encoded probes for the specific detection of each type of base substitution mutation. Using these probes, we were able to quickly determine both the mutation rate and the specificity of the mutations caused by different types of mutagens and mutagenic conditions. We also developed a PCR-based method to rapidly and robustly determine the mutation spectrum in response to various mutagenic samples in parallel. This system allows one to not only analyze the mutation specificity of various chemicals, but also to search for novel genetic elements that promote the specific mutation events.

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Section: Discussionmentioning

confidence: 99%

A System for the Rapid Determination of the Mutation Spectrum in Escherichia coli

et al. 2012

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“…The number of revertant (His+) mutant colonies after exposure with a potential carcinogen can be determined. A similar test utilizing Escherichia coli and a tryptophan synthesis reverse mutation is also commonly applied and approved by the OECD (guideline T472) [105,106]. Such tests are often applied as a preliminary test for potential carcinogenicity of substances.…”

Section: Specific Endpoints 321 Mutagenesis and Carcinogenicitymentioning

confidence: 99%

Cell-based in vitro models in environmental toxicology: a review

Poteser¹

2017

Biomonitoring

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An analysis of biological effects induced by environmental toxins and exposure-related evaluation of potential risks for health and environment represent central tasks in classical biomonitoring. While epidemiological data and population surveys are clearly the methodological frontline of this scientific field, cellbased in vitro assays provide information on toxin-affected cellular pathways and mechanisms, and are important sources for the identification of relevant biomarkers. This review provides an overview on currently available in vitro methods based on cultured cells, as well as some limitations and considerations that are of specific interest in the context of environmental toxicology. Today, a large number of different endpoints can be determined to pinpoint basal and specific toxicological cellular effects. Technological progress and increasingly refined protocols are extending the possibilities of cell-based in vitro assays in environmental toxicology and promoting their increasingly important role in biomonitoring.

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“…The E. coli WP2 (uvrA) strain carries a mutation at the tryptophan (trp) allele, which is an auxotrophic mutation reverted by base-pair substitution. The strain is deficient in the repair of UV-induced DNA damage (uvrA) (Bridges, 1972;Green and Muriel, 1976;Mortelmans and Riccio, 2000) and thus has enhanced sensitivity to some mutagenic agents.…”

Section: Test Systemmentioning

confidence: 99%