The bacterial replication initiator DnaA. DnaA and , the bacterial mode to initiate DNA replication

MESSER, W

doi:10.1016/s0168-6445(02)00127-4

Cited by 183 publications

(309 citation statements)

References 161 publications

(262 reference statements)

Supporting

Mentioning

298

Contrasting

Order By: Relevance

“…Circular representations of both chromosomes are depicted in Figure 1. The origin of replication of the large replicon was identified between the dnaA and dnaN genes, as in other bacterium genomes (10). GC nucleotide skew (G -C/G + C) analysis (11) confirmed the origin of replication of the large replicon and indicated two putative sites for the small replicon.…”

Section: Genome Analysismentioning

confidence: 69%

Genome features of Leptospira interrogans serovar Copenhageni

Nascimento

Verjovski-Almeida

Sluys

et al. 2004

Braz J Med Biol Res

199

183

View full text Add to dashboard Cite

We report novel features of the genome sequence of Leptospira interrogans serovar Copenhageni, a highly invasive spirochete. Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in mammals. Genomic sequence analysis reveals the presence of a competent transport system with 13 families of genes encoding for major transporters including a three-member component efflux system compatible with the long-term survival of this organism. The leptospiral genome contains a broad array of genes encoding regulatory system, signal transduction and methyl-accepting chemotaxis proteins, reflecting the organism's ability to respond to diverse environmental stimuli. The identification of a complete set of genes encoding the enzymes for the cobalamin biosynthetic pathway and the novel coding genes related to lipopolysaccharide biosynthesis should bring new light to the study of Leptospira physiology. Genes related to toxins, lipoproteins and several surface-exposed proteins may facilitate a better understanding of the Leptospira pathogenesis and may serve as potential candidates for vaccine.

show abstract

Section: Genome Analysismentioning

confidence: 69%

Genome features of Leptospira interrogans serovar Copenhageni

Nascimento

Verjovski-Almeida

Sluys

et al. 2004

Braz J Med Biol Res

199

183

View full text Add to dashboard Cite

show abstract

“…When searching for DnaA boxes in the chromosome of Anabaena PCC 7120, hundreds of DnaA boxes were found (data not shown), six of which were located in the intergenic non-coding region between dnaA and dnaN, and the DnaA box sequence was TTTTCCACA. The presence of a cluster of DnaA boxes in the immediate neighbourhood of the dnaA gene provides a good indication that this intergenic region (Messer, 2002). Although only four of these cyanobacterial oriC regions have the dnaA-coding region in the immediate neighbourhood (Fig.…”

Section: Prediction Of Oric In Anabaena Pcc 7120 and Comparison Of Thmentioning

confidence: 99%

“…DNA replication starts at the replication origin, oriC, which contains several DnaA boxes and an AT-rich region. The DnaA protein binds to DnaA boxes and opens the DNA duplex at the AT-rich region, and then initiates the process of DNA replication (Messer, 2002). In many bacteria, the DnaA box is an asymmetrical 9 bp recognition sequence: 59-TT A / T TNCACA-39 (Schaper & Messer, 1995).…”

Section: Introductionmentioning

confidence: 99%

Identification of the oriC region and its influence on heterocyst development in the filamentous cyanobacterium Anabaena sp. strain PCC 7120

et al. 2011

View full text Add to dashboard Cite

PCC 7120 (Anabaena PCC 7120) is a filamentous, nitrogen-fixing cyanobacterium. Upon deprivation of combined nitrogen, about 5-10 % of the cells become heterocysts, i.e. cells devoted to N 2 fixation. Heterocysts are intercalated among vegetative cells and distributed in a semi-regular pattern, and adjacent heterocysts are rarely observed. Previously, we showed that the cell cycle could play a regulatory function during heterocyst development, although the mechanism involved remains unknown. As a further step to understand this phenomenon, we identified the oriC region for chromosomal DNA replication, located between dnaA and dnaN. The oriC region of Anabaena PCC 7120 was able to support the self-replication of a plasmid in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Surprisingly, integration of the oriC region into the chromosome of Anabaena PCC 7120 through homologous recombination led to much slower cell growth in the absence of a combined-nitrogen source and to multiple contiguous proheterocysts after prolonged incubation. Real-time RT-PCR showed that expression of two heterocyst-related genes, hetR and hetN, was altered in these strains: hetR expression remained high 48 h after induction, and hetN increased to high levels after induction for 12 h. These results suggest that the balance between oriC and DnaA could be important for heterocyst development.

show abstract

“…AAA+ | bacteria | initiation regulation | in vitro reconstitution | nucleoprotein complex I n Escherichia coli, DnaA protein initiates DNA replication by forming a nucleoprotein complex with the chromosomal replication origin oriC (1)(2)(3)(4). DnaA consists of four functional domains: (i) Domain I binds to specific proteins, such as DnaB helicase.…”

mentioning

confidence: 99%

“…In E. coli, the cell cycle-coordinated initiation of chromosomal replication is sustained by multiple systems that regulate the activities of DnaA and oriC (1)(2)(3)(4)15). After ATP-DnaA induces replication initiation, DnaA-bound ATP is hydrolyzed by Hda protein in complex with the DNA-loaded β-clamp subunit of DNA polymerase III holoenzyme, yielding initiation-inactive ADPDnaA (2,4,16,17).…”

mentioning

confidence: 99%

DnaA binding locus datA promotes DnaA-ATP hydrolysis to enable cell cycle-coordinated replication initiation

Kasho

Katayama

2012

Proc. Natl. Acad. Sci. U.S.A.

102

231

View full text Add to dashboard Cite

The initiation of chromosomal DNA replication is rigidly regulated to ensure that it occurs in a cell cycle-coordinated manner. To ensure this in Escherichia coli, multiple systems regulate the activity of the replication initiator ATP-DnaA. The level of ATP-DnaA increases before initiation after which it drops via DnaA-ATP hydrolysis, yielding initiation-inactive ADP-DnaA. DnaA-ATP hydrolysis is crucial to regulation of initiation and mainly occurs by a replication-coupled feedback mechanism named RIDA (regulatory inactivation of DnaA). Here, we report a second DnaA-ATP hydrolysis system that occurs at the chromosomal site datA. This locus has been annotated as a reservoir for DnaA that binds many DnaA molecules in a manner dependent upon the nucleoid-associated factor IHF (integration host factor), resulting in repression of untimely initiations; however, there is no direct evidence for the binding of many DnaA molecules at this locus. We reveal that a complex consisting of datA and IHF promotes DnaA-ATP hydrolysis in a manner dependent on specific inter-DnaA interactions. Deletion of datA or the ihf gene increased ATP-DnaA levels to the maximal attainable levels in RIDA-defective cells. Cell-cycle analysis suggested that IHF binds to datA just after replication initiation at a time when RIDA is activated. We propose a model in which cell cycle-coordinated ATP-DnaA inactivation is regulated in a concerted manner by RIDA and datA.AAA+ | bacteria | initiation regulation | in vitro reconstitution | nucleoprotein complex

show abstract

The bacterial replication initiator DnaA. DnaA and , the bacterial mode to initiate DNA replication

Cited by 183 publications

References 161 publications

Genome features of Leptospira interrogans serovar Copenhageni

Genome features of Leptospira interrogans serovar Copenhageni

Identification of the oriC region and its influence on heterocyst development in the filamentous cyanobacterium Anabaena sp. strain PCC 7120

DnaA binding locus datA promotes DnaA-ATP hydrolysis to enable cell cycle-coordinated replication initiation

Contact Info

Product

Resources

About