The bacterial load in milk is associated with clinical severity in cases of bovine coliform mastitis

Abstract

We evaluated the relationship between the severity of coliform mastitis and bacterial load in 106 quarter milk samples. We found no significant relationship between somatic cell count and coliform bacterial load in milk in bovine clinical coliform mastitis. Results of the Cochran-Armitage test for trend in milk bacterial load proportions indicated a significant decreasing low group (P<0.001), increasing medium group (P<0.002) and increasing high group (P<0.02) with increasing clinical grade. The present study indicates that the coliform bacterial load in milk is significantly associated with clinical severity states in cases of bovine coliform mastitis, and can be a useful indicator for optimal management of this disease.

Immune response during the onset of coliform mastitis in dairy cows vaccinated with STARTVAC®

“…E. coli and Klebsiella spp. are known to be the major pathogens causing coliform mastitis (Klaas & Zadoks, 2018;Nagasawa et al, 2019). It is generally known that mastitis caused by Klebsiella spp.…”

Evaluation of a rapid coliform detection kit from clinical mastitis milk using colloidal gold nanoparticle–based immunochromatographic strips

“…We have already reported that although the clinical score for coliform mastitis is unrelated to the somatic cell count, it is related to coliform bacterial counts [ 14 ]. The results of this study were similar to those of previous reports and also showed an association between clinical scores and ICS test scores.…”

“…On the other hand, the ICS test is effective only for the identification of a specific bacterial species, but it is considered to be useful when quickly determining the causative bacterial species is necessary [ 15 ]. Coliform bacterial load in milk is significantly associated with the clinical severity of coliform mastitis in cows and is considered a useful indicator for its optimal management [ 14 ]. In the ICS test, the time for result determination was one hour, which included the time for bacteriolysis and reaction; however, we were able to obtain the results as quickly as 30–40 min for milk samples from severe cases with high coliform counts.…”

“…In our previous reports, we showed in detail the process of identification and coliform measurement in milk samples [ 14 ]. We plated each sample on a 5% sheep blood agar plate (Nissui Plate Sheep Blood Agar, Nissui Pharmaceutical Co., Tokyo, Japan) and cultured the samples aerobically at 37°C for 24 hr.…”

“…Furthermore, even if colonies are obtained from culture, skilled technicians are required for identification. Thus, to reduce the damage to cows, there is a need to develop a rapid and efficient tool to identify bacterial species in coliform-induced bovine mastitis [ 14 ].…”