The Bacterial Hemagglutination Test for the Demonstration of Antibodies to Enterobacteriaceae

Neter, Erwin; Westphal, Otto; Lüderitz, Otto; Gorzynski, E. A.

doi:10.1111/j.1749-6632.1956.tb40113.x

Cited by 107 publications

(45 citation statements)

References 17 publications

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“…Sera were stored at -15° and stool samples were stored on Dry Ice. Antibodies against E. coli 083 were determined by a hemagglutination technique on microtitrator plates according to the method of Neter et al [25].…”

Section: Methodsmentioning

confidence: 99%

Serum Immunoglobulins and Coproantibody Formation in Infants after Artificial Intestinal Colonization with Escherichia coli 083 and Oral Lysozyme Administration

1973

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Section: Methodsmentioning

confidence: 99%

Serum Immunoglobulins and Coproantibody Formation in Infants after Artificial Intestinal Colonization with Escherichia coli 083 and Oral Lysozyme Administration

1973

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“…The assay was done by the method of Neter et al using lipopolysaccharide-coated sheep red blood cells (sheep RBC) [33]. The sheep RBC were coated with lipopolysaccharide by suspending them in buffer A at a density of 5 x lo8 cells/ml, and incubating 1 ml of this suspension with 0.5 ml lipopolysaccharide solution (1 mg/ml in buffer A) at 37°C for 30 min.…”

Section: Assay Of Lipopolysaccharide-binding Proteinmentioning

confidence: 99%

Purification and characterization of lipopolysaccharide‐binding protein from hemolymph of American cockroach Periplaneta americana

Jomori

Kubo

Natori

1990

European Journal of Biochemistry

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A protein having affinity to lipopolysaccharide of Escherichia coli K12 was purified to homogeneity from the hemolymph of Periplaneta americana. This protein, with an average molecular mass of 450 kDa, was a homooligomer of a 28‐kDa subunit protein. Comparative studies using lipopolysaccharide molecules of E. coli and Salmonella minnesota suggested that this protein recognizes and binds to a specific carbohydrate structure of E. coli lipopolysaccharide. Ca2+ was required for this protein to bind to lipopolysaccharide, but other divalent cations could not replace Ca2+.

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“…The technique for coating RBC with LPS has been described (Neter et al, 1956). In our experiments the final concentration of LPS was 500 ng/ml.…”

Section: Endotoxinmentioning

confidence: 99%

“…Serum was collected each week before immunisation by puncture of the retro-orbital venous plexus and titrated against LPS-coated RBC as described (Neter et al 1956). …”

Section: Haemagglutination Testsmentioning

confidence: 99%

Failure to Induce Autoimmunity in Mice With Endotoxin

Cox

Keast

1974

Aust J Exp Biol Med

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Summary. Prolonged immunisation of mice with endotoxin-coated syngeneic erythrocytes provoked strong immunity against endotoxin. Endotoxin-coated, "''iCr-Iabelled syngeneic erythrocytes were cleared faster in immunised mice compared with unimmunised mice. The clearance of allogeneic erythrocytes was unchanged. There wa.s no autoimmune response against syngeneic erythrocytes as measured by direct Coombs' tests and in vivo survival studies of ^^Cr-labelled erythrocytes.INTRODUCTION.

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The Bacterial Hemagglutination Test for the Demonstration of Antibodies to Enterobacteriaceae

Cited by 107 publications

References 17 publications

Serum Immunoglobulins and Coproantibody Formation in Infants after Artificial Intestinal Colonization with Escherichia coli 083 and Oral Lysozyme Administration

Serum Immunoglobulins and Coproantibody Formation in Infants after Artificial Intestinal Colonization with Escherichia coli 083 and Oral Lysozyme Administration

Purification and characterization of lipopolysaccharide‐binding protein from hemolymph of American cockroach Periplaneta americana

Failure to Induce Autoimmunity in Mice With Endotoxin

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