The bacterial flora of tsetse fly midgut and its effect on trypanosome transmission

Soumana, Illiassou Hamidou; Simo, Gustave; Njiokou, Flobert; Tchicaya, Bernadette; Abd-Alla, Adly M. M.; Cuny, G; Geiger, Anne

doi:10.1016/j.jip.2012.03.029

Cited by 38 publications

(21 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These experiments were performed on the midguts of insectary-reared Gpg flies. As previously shown, these flies harbor the obligate symbiont W. glossinidia and the secondary symbiont S. glossinidius (Hamidou Soumana et al, 2013 ). We first analyzed the proteomes of midguts from flies that received either a trypanosome-infected or non-infected bloodmeal.…”

Section: Introductionmentioning

confidence: 73%

Differential expression of midgut proteins in Trypanosoma brucei gambiense-stimulated vs. non-stimulated Glossina palpalis gambiensis flies

et al. 2015

Self Cite

View full text Add to dashboard Cite

The unicellular pathogenic protozoan Trypanosoma brucei gambiense is responsible for the chronic form of sleeping sickness. This vector-borne disease is transmitted to humans by the tsetse fly of the group Glossina palpalis, including the subspecies G. p. gambiensis, in which the parasite completes its developmental cycle. Sleeping sickness control strategies can therefore target either the human host or the fly vector. Indeed, suppression of one step in the parasite developmental cycle could abolish parasite transmission to humans, with consequences on the spreading of the disease. In order to develop this type of approach, we have identified, at the proteome level, events resulting from the tripartite interaction between the tsetse fly G. p. gambiensis, its microbiome, and the trypanosome. Proteomes were analyzed from four biological replicates of midguts from flies sampled 3 days post-feeding on either a trypanosome-infected (stimulated flies) or a non-infected (non-stimulated flies) bloodmeal. Over 500 proteins were identified in the midguts of flies from both feeding groups, 13 of which were shown to be differentially expressed in trypanosome-stimulated vs. non-stimulated flies. Functional annotation revealed that several of these proteins have important functions that could be involved in modulating the fly infection process by trypanosomes (and thus fly vector competence), including anti-oxidant and anti-apoptotic, cellular detoxifying, trypanosome agglutination, and immune stimulating or depressive effects. The results show a strong potential for diminishing or even disrupting fly vector competence, and their application holds great promise for improving the control of sleeping sickness.

show abstract

Section: Introductionmentioning

confidence: 73%

Differential expression of midgut proteins in Trypanosoma brucei gambiense-stimulated vs. non-stimulated Glossina palpalis gambiensis flies

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…While the fecundity of these flies does not change, they become more resistant to trypanosome infections (Dale and Welburn, 2001). In G. palpalis populations collected in Cameroon, parasite infection prevalence positively correlated with the presence of Sodalis in examined flies (Farikou et al, 2010; Soumana et al, 2013). These results suggest that in contrast to Wigglesworthia , which increases tsetse refractoriness to trypanosomes, Sodalis appears to favor the establishment of trypanosome infections in tsetse.…”

Section: The Biology Of Tsetse Symbiosismentioning

confidence: 99%

Tsetse fly microbiota: form and function

Wang

Weiss

Aksoy

2013

Front. Cell. Infect. Microbiol.

113

View full text Add to dashboard Cite

Tsetse flies are the primary vectors of African trypanosomes, which cause Human and Animal African trypanosomiasis in 36 countries in sub-Saharan Africa. These flies have also established symbiotic associations with bacterial and viral microorganisms. Laboratory-reared tsetse flies harbor up to four vertically transmitted organisms—obligate Wigglesworthia, commensal Sodalis, parasitic Wolbachia and Salivary Gland Hypertrophy Virus (SGHV). Field-captured tsetse can harbor these symbionts as well as environmentally acquired commensal bacteria. This microbial community influences several aspects of tsetse's physiology, including nutrition, fecundity and vector competence. This review provides a detailed description of tsetse's microbiome, and describes the physiology underlying host-microbe, and microbe-microbe, interactions that occur in this fly.

show abstract

“…1), but is predominantly found in the midgut [89]. The function of Sodalis in tsetse is poorly understood, and the degree to which and how Sodalis contributes to tsetse vector competency remain a contentious topic [90]. One theory proposes that the chitinolytic activity of Sodalis within tsetse’s midgut may potentiate trypanosome infection susceptibility in teneral (newly eclosed) flies through the release of N-acetyl-d-glucosamine (GlcNAc) and its inhibition of anti-trypanosomal lectins [91, 92] found naturally in low abundance in young adult flies.…”

Section: Tsetse Flymentioning

confidence: 99%

Grandeur Alliances: Symbiont Metabolic Integration and Obligate Arthropod Hematophagy

Rio

Attardo

Weiss

2016

Trends in Parasitology

102

View full text Add to dashboard Cite

Several arthropod taxa live exclusively on vertebrate blood. This food source lacks essential metabolites required for the maintenance of metabolic homeostasis, and as such, these arthropods have formed symbioses with nutrient-supplementing microbes that facilitate their host’s ‘hematophagous’ feeding ecology. Herein we highlight metabolic contributions of bacterial symbionts that reside within tsetse flies, bed bugs, lice, reduviid bugs and ticks, with specific emphasis on B vitamin and cofactor biosynthesis. Importantly, these arthropods can transmit pathogens of medical and veterinary relevance and/or cause infestations that induce psychological and dermatological distress. Microbial metabolites, and the biochemical pathways that generate them, can serve as specific targets of novel control mechanisms aimed at disrupting the metabolism of hematophagous arthropods, thus combatting pest invasion and vector-borne pathogen transmission.

show abstract

The bacterial flora of tsetse fly midgut and its effect on trypanosome transmission

Cited by 38 publications

References 51 publications

Differential expression of midgut proteins in Trypanosoma brucei gambiense-stimulated vs. non-stimulated Glossina palpalis gambiensis flies

Differential expression of midgut proteins in Trypanosoma brucei gambiense-stimulated vs. non-stimulated Glossina palpalis gambiensis flies

Tsetse fly microbiota: form and function

Grandeur Alliances: Symbiont Metabolic Integration and Obligate Arthropod Hematophagy

Contact Info

Product

Resources

About