The bacterial flagellin gene as a biomarker for detection, population genetics and epidemiological analysis

Winstanley, Craig; Morgan, J. Alun W.

doi:10.1099/00221287-143-10-3071

Cited by 104 publications

(96 citation statements)

References 85 publications

(100 reference statements)

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“…Flagellin proteins have a distinctive domain structure, comprising conserved N-and C-terminal regions, and a central domain that may vary considerably in both amino acid sequence and size (Macnab 1992, Winstanley & Morgan 1997. In Escherichia coli, flagellin proteins are encoded by the fliC gene that has conserved 5' and 3' regions and a high polymorphic central region (Kuwajima et al 1986, Winstanley & Morgan 1997, Fields et al 1997, Wang et al 2003, Botelho et al 2003, Amhaz et al 2004, Tominaga 2004, Beutin et al 2005, Moreno et al 2006.…”

Section: Introductionmentioning

confidence: 99%

“…In Escherichia coli, flagellin proteins are encoded by the fliC gene that has conserved 5' and 3' regions and a high polymorphic central region (Kuwajima et al 1986, Winstanley & Morgan 1997, Fields et al 1997, Wang et al 2003, Botelho et al 2003, Amhaz et al 2004, Tominaga 2004, Beutin et al 2005, Moreno et al 2006. Joys (1985) suggested that the accumulation of random mutations maintained by functional pressure absence is responsible by the polymorphism presented in the central region of fliC gene.…”

Section: Introductionmentioning

confidence: 99%

“…Flagellar activity is coupled to a sensory apparatus in a control system that allows movement of a cell towards attractive environments and away from repellent ones. The basic structure of bacterial flagella can be sub divided into three parts: (i) the basal body, which consists of a series of rings and a central rod, is anchored in the inner and outer membranes of the cell; (ii) the hook is located external to the cell and provides the link between the basal body and filament; and (iii) the flagellar filament, the largest portion of the flagella, consists of repeating sub-units of the protein flagellin in a helical arrangement and that often extends many times the length of the cell (Macnab 1992, Winstanley & Morgan 1997.…”

Section: Introductionmentioning

confidence: 99%

“…Among surface bacterial cell proteins, flagella proteins have been used as a target to population variation studies (Winstanley & Morgan 1997).…”

Section: Introductionmentioning

confidence: 99%

“…The fliC gene has been used in bacterial systematic and population genetic studies because its variability reflects the selective procedures involved on the bacterial flagella antigen (H antigen) diversity. For phylogenetic trees and intra-specific population variation analysis the fliC sequences are divided in three regions: two corresponding to the 5' and 3' conserved regions and one corresponding to the central region (Winstanley & Morgan 1997).…”

Section: Introductionmentioning

confidence: 99%

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Clonal study of avian Escherichia coli strains by fliC conserved-DNA-sequence regions analysis

et al. 2008

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ABSTRACT.-Campos T.A., Nakazato G., Stehling E.G., The clonal relationship among avian Escherichia coli strains and their genetic proximity with human pathogenic E. coli, Salmonela enterica, Yersinia enterocolitica and Proteus mirabilis, was determined by the DNA sequencing of the conserved 5' and 3'regions fliC gene (flagellin encoded gene). Among 30 commensal avian E. coli strains and 49 pathogenic avian E. coli strains (APEC), 24 commensal and 39 APEC strains harbored fliC gene with fragments size varying from 670bp to 1,900bp. The comparative analysis of these regions allowed the construction of a dendrogram of similarity possessing two main clusters: one compounded mainly by APEC strains and by H-antigens from human E. coli, and another one compounded by commensal avian E. coli strains, S. enterica, and by other H-antigens from human E. coli. Overall, this work demonstrated that fliC conserved regions may be associated with pathogenic clones of APEC strains, and also shows a great similarity among APEC and H-antigens of E. coli strains isolated from humans. These data, can add evidence that APEC strains can exhibit a zoonotic risk. Clonal study of avian Escherichia coli strains by fliC conserved-DNA-sequence regions analysis

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Among surface bacterial cell proteins, flagella proteins have been used as a target to population variation studies (Winstanley & Morgan 1997).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Clonal study of avian Escherichia coli strains by fliC conserved-DNA-sequence regions analysis

et al. 2008

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Bioinformatics for Flexibility, Reliability, and Mixture Analysis of Intact Microorganisms

Fenselau

Pribil

2005

Identification of Microorganisms by Mass Spectrometry

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Enhanced CBir1-specific innate and adaptive immune responses in Crohnʼs disease

Shen

Landers

Derkowski

et al. 2008

Inflammatory Bowel Diseases

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CBir1 is a dominant antigen with a role in innate and adaptive immunity in mouse models of colitis and antibodies to CBir1 are associated with severe human Crohn’s disease. Our aim was to determine whether CBir1 stimulates innate and antigen-specific T-cell responses in Crohn’s disease. We demonstrate that CBir1 enhanced IL-6 and IL-1β production by peripheral blood (PB) monocytes. IL-6 was significantly increased in monocytes from Crohn’s disease compared to controls and ulcerative colitis. Anti-CBir1+ patients and IL-6 was inversely correlated. A significant increase in CBir1 specific peripheral T cell proliferation was more evident in cells from Crohn’s disease than controls and ulcerative colitis. CBir1 induced increased numbers of IFN-gamma+ cells in lamina propria mononuclear cells (LPMC) from Crohn’s disease compared to ulcerative colitis and controls. CBir1 induces enhanced peripheral innate, and peripheral and mucosal antigen-specific T-cell responses in Crohn’s disease. Consistent with results from the mouse, CBir1 immune activation could play a role in Crohn’s disease.

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The bacterial flagellin gene as a biomarker for detection, population genetics and epidemiological analysis

Cited by 104 publications

References 85 publications

Clonal study of avian Escherichia coli strains by fliC conserved-DNA-sequence regions analysis

Clonal study of avian Escherichia coli strains by fliC conserved-DNA-sequence regions analysis

Bioinformatics for Flexibility, Reliability, and Mixture Analysis of Intact Microorganisms

Enhanced CBir1-specific innate and adaptive immune responses in Crohnʼs disease

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