The bacterial composition associated with Atriolum robustum, a common ascidian from Xisha coral reef, China

Liu, Yong; Wu, Peng; Li, Chunhou; Xiao, Yao; Wang, Teng; Lin, Lin

doi:10.1007/s13199-020-00742-4

Cited by 3 publications

(2 citation statements)

References 45 publications

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“…As in many other sedentary marine organisms, symbiotic bacteria are known to reside in tunicates and are also known as tissue-associated, such as in endodermal tissues, the pharynx, the tunic and the intestine ( Behrendt et al, 2012 ; Dishaw et al, 2014 ). The ascidian microbiota contributes to nutrition via photosynthesis ( Donia et al, 2011 ; López-Legentil et al, 2011 ; Hirose, 2015 ), production of secondary metabolites ( Schmidt, 2014 ; Tianero et al, 2015 ; Chen et al, 2018 ), nitrification ( Martínez-García et al, 2008 ; Erwin et al, 2014 ), protection against biofouling, predation ( Degnan et al, 1989 ; Paul et al, 1990 ; Fu et al, 1998 ; Vervoort et al, 1998 ; Faulkner, 2001 ; Schreiber et al, 2016 ), vanadium accumulation ( Ueki et al, 2019 ), and the host immune response ( Liu et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Physiological changes during torpor favor association with Endozoicomonas endosymbionts in the urochordate Botrylloides leachii

et al. 2023

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Environmental perturbations evoke down-regulation of metabolism in some multicellular organisms, leading to dormancy, or torpor. Colonies of the urochordate Botrylloides leachii enter torpor in response to changes in seawater temperature and may survive for months as small vasculature remnants that lack feeding and reproductive organs but possess torpor-specific microbiota. Upon returning to milder conditions, the colonies rapidly restore their original morphology, cytology and functionality while harboring re-occurring microbiota, a phenomenon that has not been described in detail to date. Here we investigated the stability of B. leachii microbiome and its functionality in active and dormant colonies, using microscopy, qPCR, in situ hybridization, genomics and transcriptomics. A novel lineage of Endozoicomonas, proposed here as Candidatus Endozoicomonas endoleachii, was dominant in torpor animals (53–79% read abundance), and potentially occupied specific hemocytes found only in torpid animals. Functional analysis of the metagenome-assembled genome and genome-targeted transcriptomics revealed that Endozoicomonas can use various cellular substrates, like amino acids and sugars, potentially producing biotin and thiamine, but also expressing various features involved in autocatalytic symbiosis. Our study suggests that the microbiome can be linked to the metabolic and physiological states of the host, B. leachii, introducing a model organism for the study of symbioses during drastic physiological changes, such as torpor.

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Section: Introductionmentioning

confidence: 99%

Physiological changes during torpor favor association with Endozoicomonas endosymbionts in the urochordate Botrylloides leachii

et al. 2023

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“…Coral reefs are often called the tropical rainforests of the ocean and are the most productive and biologically diverse ecosystem in the world, supporting more than 25% of all known marine species (Moberg and Folke 1999;Walker and Wood, 2005). However, the world's coral reefs are in serious decline due to numerous anthropogenic activities and global climate disturbances (Wilkinson, 2008;Liu et al 2021). Nineteen percent of the world's coral reefs were lost in the 4 years of [2004][2005][2006][2007][2008], and only 46% of the world's reefs were considered to be in a relatively healthy condition during that period (Wilkinson, 2008).…”

Section: Introductionmentioning

confidence: 99%

Characteristics of Macroalgal Consumption by Eight Herbivorous Coral Reef Fishes From the Xisha Islands, China, as Determined by Microscopy, 18S rRNA High-throughput Sequencing and Stable Isotope Analyses

Wu¹,

Wang²,

Liu³

et al. 2021

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Herbivorous fishes play an important role in controlling the overabundance of macroalgae on coral reefs. Understanding the feeding selectivity and consumption of macroalgae by herbivorous fishes can be challenging in studies of their ecological role in the preservation and recovery of coral reefs. Coral reef decline, macroalgal overgrowth and overfishing are clearly visible in the Xisha Islands, China. However, there have been no studies of the feeding behaviors of herbivorous fishes in this area. We used microscopy, 18S rRNA high-throughput sequencing and stable isotope analyses to comprehensively examine the dietary spectrum of eight herbivorous reef fish species common in the Xisha Islands, including one parrotfish, two chub, two unicorn fish and three rabbitfish. Multi-technique analyses of intestinal contents revealed that Kyphosus vaigiensis, Naso unicornis and Siganus argenteus showed a high consumption potential of macroalgae, suggesting that they are the key browsers which should receive priority protection in in the Xisha Islands. Kyphosus cinerascens, K. vaigiensis, N. unicornis and S. punctatissimus fed on the entire macroalgal thallus, indicating their greater ecological importance compared with species which only consume the algal fronds. However, Calotomus carolinus can consume the red alga Pneophyllum conicum, which is widely distributed on Indo-Pacific coral reefs and can overgrow and kill live corals. Clearly, a diverse herbivorous fish fauna is very important in the Xisha coral reefs. These results not only demonstrated the various functions of different herbivorous fish species in macroalgal removal, but also provided insights into the management of herbivorous fishes on the coral reefs of the South China Sea.

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Differences of Macroalgal Consumption by Eight Herbivorous Coral Reef Fishes From the Xisha Islands, China

Wang

Liu

et al. 2022

Front. Mar. Sci.

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Herbivorous fishes play an important role in preventing the overabundance of macroalgae on coral reefs. Understanding the feeding selectivity and consumption of macroalgae by herbivorous fishes can be challenging in studies of their ecological role in the preservation and recovery of coral reefs. Coral reef decline, macroalgal overgrowth and overfishing are clearly visible in the Xisha Islands, China. However, there are seldom studies on the feeding behaviors of herbivorous fishes in this area. We used microscopy, 18S rRNA high-throughput sequencing, and stable isotope analyses to comprehensively examine the diet of eight herbivorous reef fish species common in the Xisha Islands, including one parrotfish, two chub, two unicorn fish, and three rabbitfish. Based on microscopic examination, Siganus argenteus fed on the highest number of macroalgae species (five species), followed by Naso unicornis (four species). Kyphosus cinerascens, K. vaigiensis, N. unicornis and S. punctatissimus fed on the entire macroalgal thallus, indicating their greater ecological importance compared with species that only consume algal fronds. According to the 18S rRNA high-throughput sequencing of fish intestinal contents, cluster analysis revealed that consumed macroalgae composition from the eight fishes always grouped together based on the fish species, but with low similarity. K. vaigiensis removed the highest diversity of macroalgae species as well as the greatest quantity of macroalgae. Calotomus carolinus can consume the red alga Pneophyllum conicum, which is widely distributed on Indo-Pacific coral reefs and can overgrow and kill live corals. N. unicornis was found to occupy the lowest trophic position based on stable isotope analysis. Multi-technique analyses revealed that K. vaigiensis, N. unicornis and S. argenteus showed a high consumption potential of macroalgae, suggesting that they are the key browsers that should receive priority protection in the Xisha Islands. A diverse herbivorous fish fauna is also very important in the Xisha coral reefs. These results not only demonstrated the various functions of different herbivorous fish species in macroalgal removal, but also provided insights into the management of herbivorous fishes on the coral reefs of the South China Sea.

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The bacterial composition associated with Atriolum robustum, a common ascidian from Xisha coral reef, China

Cited by 3 publications

References 45 publications

Physiological changes during torpor favor association with Endozoicomonas endosymbionts in the urochordate Botrylloides leachii

Physiological changes during torpor favor association with Endozoicomonas endosymbionts in the urochordate Botrylloides leachii

Characteristics of Macroalgal Consumption by Eight Herbivorous Coral Reef Fishes From the Xisha Islands, China, as Determined by Microscopy, 18S rRNA High-throughput Sequencing and Stable Isotope Analyses

Differences of Macroalgal Consumption by Eight Herbivorous Coral Reef Fishes From the Xisha Islands, China

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