The baboon (Papio sp.) as a model for female reproduction studies

Bauer, Cassondra

doi:10.1016/j.contraception.2015.06.007

Cited by 34 publications

(27 citation statements)

References 53 publications

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“…At least 3 days per week, females underwent visual evaluation of sex skin tumescence to assess menstrual cycle phase and evidence of mating (semen plug) [12].…”

Section: Methodsmentioning

confidence: 99%

Transcervical administration of polidocanol foam prevents pregnancy in female baboons

et al. 2016

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BackgroundOur objective was to conduct a pilot study to determine if transcervical administration of polidocanol foam (PF) with or without doxycycline or benzalkonium chloride (BZK) would prevent pregnancy in baboons.MethodsIn study phase 1, adult cycling baboons underwent a hysterosalpingogram to evaluate tubal patency prior to transcervical infusion of 20 mL of 5% PF followed by 1 mL of saline containing 100 mg doxycycline (5%/doxy; n=5), 3% PF plus doxycycline (3%/doxy; n=4), 3% PF with 0.01% BZK (3%/BZK; n=4) or no additional treatment (control; n=9). Immediately following treatment, animals received intramuscular depot medroxyprogesterone acetate (DMPA, 2 mg/kg) to suppress cyclicity during healing and were then socially housed with males of proven fertility. The primary outcome was pregnancy within six cycles of resumption of menses (efficacy phase 1). During study phase 2, PF-treated females from study phase 1 contributed additional cycles (6–8) of exposure (efficacy phase 2), and 5 control females who had recovered from medical abortion (after study phase 1 pregnancy) were subsequently treated with 5% PF (with DMPA) and exposed to breeding (efficacy phase 1; n=3 six cycles, n=2 five cycles).ResultsAll females resumed normal menstrual cycles and mating activity after DMPA. During efficacy phase 1, 7/9 (78%) control females became pregnant. In contrast, fewer pregnancies occurred in PF-treated females: 5% PF 0/5 (0%), 5%/doxy 1/5 (20%), 3%/doxy 1/4 (25%) and 3%/BZK 1/4 (25%). During efficacy phase 2, only one additional pregnancy occurred (3%/BZK).ConclusionsA single transcervical treatment with 5% PF prevented pregnancy in most baboons. Cotreatment with doxycycline or BZK did not improve results.ImplicationsTranscervical intrauterine administration of PF resulted in a high rate of tubal occlusion with prevention of pregnancy; refinements are needed to increase the contraceptive rate following a single treatment to near 100%.

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“…At least 3 days per week, females underwent visual evaluation of sex skin tumescence to assess menstrual cycle phase and evidence of mating (semen plug) [12].…”

Section: Methodsmentioning

confidence: 99%

Transcervical administration of polidocanol foam prevents pregnancy in female baboons

et al. 2016

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“…In an attempt to understand the physiopathology of Brucella-induced obstetric complications in women, we investigated the consequences of infection on human trophoblasts using as a model B. melitensis, the most common pathogenic species in human brucellosis. We also used B. papionis (Schlabritz-Loutsevitch et al, 2009) because this species was associated with pregnancy complications in primates whose reproductive system is highly similar to that in humans (Bauer, 2015). We show that B. papionis can infect human CTB, EVT, and SYN.…”

Section: Introductionmentioning

confidence: 96%

“…We also used B . papionis (Schlabritz‐Loutsevitch et al, ) because this species was associated with pregnancy complications in primates whose reproductive system is highly similar to that in humans (Bauer, ). We show that B .…”

Section: Introductionmentioning

confidence: 99%

“…Because Papio sp. are widely used as an animal model to study human reproduction (including placental infection-related pathologies;Onditi et al, 2015) because of their high similarities in placental structure and fetal development(Bauer, 2015), infection with B. papionis in these primatesmight constitute a relevant in vivo model to study Brucella-induced placental disorders. Interestingly, B. papionis replicates actively within human CTB or SYN but only survives in EVT.…”

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confidence: 99%

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Infection by Brucella melitensis or Brucella papionis modifies essential physiological functions of human trophoblasts

García‐Méndez

Hielpos

Soler-Lloréns

et al. 2019

Cellular Microbiology

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Brucellosis is a zoonosis caused by bacteria of the Brucella genus. In ruminants, brucellosis causes abortion, followed by chronic infection and secretion of bacteria in milk. In humans, it usually presents as flu‐like symptoms, with serious complications if untreated. Epidemiological studies have only recently established that brucellosis can also cause pregnancy complications in women, but the pathogenic mechanisms are unknown. Pioneering studies in ruminants showed that Brucella infect trophoblasts and then colonise the placenta where they grow to high density. A recent study showed that the main zoonotic Brucella species can infect human cytotrophoblasts (CTB) and extravillous trophoblasts (EVT). In this work, we show that Brucella papionis (associated with stillbirth in primates) also infects human trophoblasts. However, it replicates actively in CTB, whereas its replication is very restricted within EVT. We also observed alteration of several trophoblastic functions upon infection by B. papionis or Brucella melitensis (the most prevalent species in human brucellosis). Infection altered the production of hormones, the ability of CTB to form syncytiotrophoblasts, and the invasion capacity of EVT. We also found that infection can spread between different types of trophoblasts. These findings constitute a new step in understanding how Brucella infection causes adverse pregnancy outcomes.

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“…In contrast, the normal baboon vaginal microbiota is diverse and reminiscent of a BV state, although they do not show symptoms of BV such as discharge or odor [3]. A NHP model for Cu-IUDs would permit studies regarding the impact of Cu-IUDs on the biology of the female reproductive tract [4] and the relationship between intrauterine contraception and infectious agents ( e.g. Chlamydia trachomatis , HIV) that may be ethically impossible in humans.…”

Section: Introductionmentioning

confidence: 99%

Effects of the copper IUD on composition of the vaginal microbiota in the olive baboon

Eastman

Sack

Chai

et al. 2019

Preprint

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ObjectivesAssess the impact of transcervical insertion, use, and removal of copper intrauterine devices (Cu-IUD) on baboon physiology (e.g. weight, menstruation) and vaginal microbiota.Study designVaginal swabs were taken before insertion (pre-IUD), during IUD use (IUD), and after removal (post-IUD) and microbiota assessed by 16S rRNA-encoding gene sequence analysis.ResultsNo animals showed physical changes or discomfort during pre-IUD, IUD, or post-IUD phases. There were no changes to the microbiome associated with insertion or use of Cu-IUD over 16 weeks, although removal resulted in perturbation to community structure.ConclusionsBaboons tolerate Cu-IUD insertion with minimal device-associated changes to their vaginal microbiome throughout use and have no significant changes to their physiology or menstrual cycle during any phase.ImplicationsA baboon model of Cu-IUD may allow investigations into the intersection of Cu-IUDs, reproductive tract disorders and pathogens that would not be possible in human studies.

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The baboon (Papio sp.) as a model for female reproduction studies

Cited by 34 publications

References 53 publications

Transcervical administration of polidocanol foam prevents pregnancy in female baboons

Transcervical administration of polidocanol foam prevents pregnancy in female baboons

Infection by Brucella melitensis or Brucella papionis modifies essential physiological functions of human trophoblasts

Effects of the copper IUD on composition of the vaginal microbiota in the olive baboon

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