The B3-Domain Transcription Factor VAL1 Regulates the Floral Transition by Repressing <i>FLOWERING LOCUS T</i>

Jing, Yanjun; Guo, Qiang; Lin, Rongcheng

doi:10.1104/pp.19.00642

Cited by 35 publications

(28 citation statements)

References 72 publications

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“…HSI2 has been shown to regulate the developmental transition from seeds to seedlings, at least in part, by directly silencing the expression of the embryogenesis-promoting gene AGL15 (Chen et al, 2018). HSI2 also affects the switch from vegetative growth to flowering by the down-regulation of FLC and FLOWERING LOCUS T (FT) (Qüesta et al, 2016; Yuan et al, 2016; Jing et al, 2019). In these cases, HSI2 was shown to interact, through its B3 DNA binding domain, with a pair of RY elements, located in the proximal promoters of AGL15 , the first intron of FLC or the second intron of FT .…”

Section: Discussionmentioning

confidence: 99%

HSI2/VAL1 and HSL1/VAL2 function redundantly to regulate seed dormancy by controllingDOG1expression in Arabidopsis

Allen

Chen

Wang

et al. 2019

Preprint

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27 28 DELAY OF GERMINATION1 (DOG1) represents a major quantitative locus for the genetic 29 regulation of seed dormancy in Arabidopsis. Accumulation of DOG1 in seeds leads to deep 30 dormancy and delayed germination. Here, we report that the conserved B3 DNA binding 31 domains of the transcriptional repressors HIGH-LEVEL EXPRESSION OF SUGAR 32 INDICIBLE GENE2/ VIVIPAROUS-1/ABSCISIC ACID INSENSITIVE 3-LIKE1 33 (HSI2/VAL1) and HSI2-LIKE1/ VIVIPAROUS-1/ABSCISIC ACID INSENSITIVE 3-LIKE2 34(HSL1/VAL2), which play critical roles in the developmental transition from seed maturation to 35 seedling growth, interact with RY elements in the DOG1 proximal promoter leading to 36 repression of DOG1 transcription during germination and seedling establishment. DOG1 37 expression is partially de-repressed in hsi2/val1 (hsi2-2) but not in hsl1/val2 (hsl1-1) knockout 38 mutants and is strongly upregulated in a hsi2/val1 hsl1/val2 double mutant, indicating that 39 HSI2/VAL1 and HSL1/VAL2 act redundantly to repress DOG1 expression. HSI2/VAL1 and 40 HSL1/VAL2 form homo-and hetero-dimers in vivo, and dimerization is dependent on the 41 HSI2/VAL1 PHD-like domain. Complementation of hsi2-2 with HSI2/VAL1 harboring a 42 disrupted plant homeodomain (PHD)-like domain results in stronger de-repression of DOG1 43 expression than the hsi2-2 knockout, indicating that the PHD-like domain plays a critical role in 44 mediating functional interactions between HSI2/VAL1 and HSL1/VAL2. Both HSI2/VAL1 and 45 HSL1/VAL2 interact with components of polycomb repressive complex 2 (PRC2), including 46 CURLY LEAF and MULTICOPY SUPPRESSOR OF IRA1 (MSI1), along with LIKE 47 HETERCHROMATIN PROTEIN 1 (LHP1), which are involved in the deposition and expansion 48 of histone H3 lysine 27 trimethylation (H3K27me3) marks in repressive chromatin. Thus, 49 HSI2/VAL1 HSL1/VAL2-dependent recruitment of PRC2 leads to silencing of DOG1 through 50 the deposition of H3K27me3.51 52 Transcriptional repression 54 55 109 to positively recruit PRC2 to chromatin for the deposition of H3K27me3 (Derkacheva et al., 110 2013). LHP1 also directly interacts with RING-RAWUL proteins and EMBRYONIC 111 FLOWER1 (EMF1, a component of PRC1), suggesting LHP1 can be present in several PRC1-112 like complexes and may act as a bridge between PRC1 and PRC2 (Xu et al., 2008; Bratzel et al., 113 2010). In Arabidopsis, LHP1 co-localizes with H3K27me3 across the genome, and is responsible 114 for the expansion of H3K27me3 associated with the stabilization of transcriptional repression 115 ( Turck et al., 2007; Zhang et al., 2007; Exner et al., 2009). Expression of many tissue specific 116 genes is upregulated in lhp1 loss-of-function mutants (Lafos et al., 2011; Libault et al., 2005), 117 5 with decreased levels of H3K27me3 seen at direct target gene loci, including FLC ( Yuan et al., 118 2016; Veluchamy et al., 2016), indicating that LHP1-dependent gene repression correlates with 119 deposition of H3K27me3. Molitor et al. (2014) reported that DOG1 is negatively regulated by 120 ALFIN1-like prot...

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Section: Discussionmentioning

confidence: 99%

HSI2/VAL1 and HSL1/VAL2 function redundantly to regulate seed dormancy by controllingDOG1expression in Arabidopsis

Allen

Chen

Wang

et al. 2019

Preprint

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“…VAL1 interacts with AtBMI1A and AtBMI1B, the components of plant PRC1, to initiate H2A lysine 121 ubiquitination (H2A121ub) at several seed maturation genes ( 30 ). VAL1 binds to the RY element (CATGCA/TGCATG) via its B3 domain ( 20–23 , 32 , 33 ), and mutation of the RY element in the first intron of the floral repressor FLOWERING LOCUS C ( FLC ) leads to decreased H3K27me3 accumulation at FLC ( 22 , 23 ). Furthermore, val1 val2 mutant seedlings show a reduction in H3K27me3 deposition at FLC ( 22 , 23 ).…”

Section: Introductionmentioning

confidence: 99%

The transcriptional repressors VAL1 and VAL2 recruit PRC2 for genome-wide Polycomb silencing in Arabidopsis

Yuan

Song

Zhang

et al. 2020

Nucleic Acids Research

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The Polycomb repressive complex 2 (PRC2) catalyzes histone H3 Lys27 trimethylation (H3K27me3) to repress gene transcription in multicellular eukaryotes. Despite its importance in gene silencing and cellular differentiation, how PRC2 is recruited to target loci is still not fully understood. Here, we report genome-wide evidence for the recruitment of PRC2 by the transcriptional repressors VIVIPAROUS1/ABI3-LIKE1 (VAL1) and VAL2 in Arabidopsis thaliana. We show that the val1 val2 double mutant possesses somatic embryonic phenotypes and a transcriptome strikingly similar to those of the swn clf double mutant, which lacks the PRC2 catalytic subunits SWINGER (SWN) and CURLY LEAF (CLF). We further show that VAL1 and VAL2 physically interact with SWN and CLF in vivo. Genome-wide binding profiling demonstrated that they colocalize with SWN and CLF at PRC2 target loci. Loss of VAL1/2 significantly reduces SWN and CLF enrichment at PRC2 target loci and leads to a genome-wide redistribution of H3K27me3 that strongly affects transcription. Finally, we provide evidence that the VAL1/VAL2–RY regulatory system is largely independent of previously identified modules for Polycomb silencing in plants. Together, our work demonstrates an extensive genome-wide interaction between VAL1/2 and PRC2 and provides mechanistic insights into the establishment of Polycomb silencing in plants.

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“…In addition to FLC-based LHP1 recruitment, the transcriptional repressor VIVIPAROUS1/ABSCISIC ACID INSENSITIVE3-LIKE1 (VAL1) recognizes the intronic cis-regulatory DNA element and recruits LHP1 to FT chromatin, leading to an H3K27me3 peak at FT (Y. Jing et al, 2019a) (Fig. 4).…”

Section: Regulation Of Ft Expression By Chromatin Modificationsmentioning

confidence: 99%

Transcriptional regulatory network of the light signaling pathways

Jing

Lin

2020

New Phytologist

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The developmental program by which plants respond is tightly controlled by a complex cascade in which photoreceptors perceive and transduce the light signals that drive signaling processes and direct the transcriptional reprogramming, yielding specific cellular responses. The molecular mechanisms involved in the transcriptional regulation include light-regulated nuclear localization (the phytochromes and UVR8) and nuclear accumulation (the cryptochrome, cry2) of photoreceptors. This regulatory cascade also includes master regulatory transcription factors (TFs) that bridge photoreceptor activation with chromatin remodeling and regulate the expression of numerous light-responsive genes. Light signaling-related TFs often function as signal convergence points in concert with TFs in other signaling pathways to integrate complex endogenous and environmental cues that help the plant adapt to the surrounding environment. Increasing evidence suggests that chromatin modifications play a critical role in regulating lightresponsive gene expression and provide an additional layer of light signaling regulation. Here, we provide an overview of our current knowledge of the transcriptional regulatory network involved in the light response, particularly the roles of TFs and chromatin in regulating light-responsive gene expression.

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The B3-Domain Transcription Factor VAL1 Regulates the Floral Transition by Repressing FLOWERING LOCUS T

Cited by 35 publications

References 72 publications

HSI2/VAL1 and HSL1/VAL2 function redundantly to regulate seed dormancy by controllingDOG1expression in Arabidopsis

HSI2/VAL1 and HSL1/VAL2 function redundantly to regulate seed dormancy by controllingDOG1expression in Arabidopsis

The transcriptional repressors VAL1 and VAL2 recruit PRC2 for genome-wide Polycomb silencing in Arabidopsis

Transcriptional regulatory network of the light signaling pathways

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