The
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            Nuclear Restorer Gene of Male-Sterile T-Cytoplasm Maize

Cui, Xiangqin; Wise, Roger P.; Schnable, Patrick S.

doi:10.1126/science.272.5266.1334

Cited by 360 publications

(220 citation statements)

References 34 publications

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“…As Mg 2+ is a critical cofactor of ATP synthases and other enzymes in the respiration processes, Mg 2+ level should serve as an essential regulatory element of mitochondrial activity. As mitochondrial defects have long been implicated in male sterility, it is not fortuitous that AtMGT5 plays an essential role in pollen development [26][27][28][29]. Although due to technical difficulty the sub-cellular localization of AtMGT9 protein has not been determined, it stands as a possibility that this transporter may also be localized in the mitochondria and regulate Mg 2+ homeostasis in this organelle.…”

Section: Discussionmentioning

confidence: 99%

Magnesium transporter AtMGT9 is essential for pollen development in Arabidopsis

Chen

Liu

et al. 2009

Cell Res

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Magnesium (Mg 2+ ) is abundant in plant cells and plays a critical role in many physiological processes. A 10-member gene family AtMGT (also known as AtMRS2) was identified in Arabidopsis, which belongs to a eukaryote subset of the CorA superfamily, functioning as Mg 2+ transporters. Some family members (AtMGT1 and AtMGT10) function as high-affinity Mg 2+ transporter and could complement bacterial mutant or yeast mutant lacking Mg 2+ transport capability. Here we report an AtMGT family member, AtMGT9, that functions as a low-affinity Mg 2+ transporter, and is essential for pollen development. The functional complementation assay in Salmonella mutant strain MM281 showed that AtMGT9 is capable of mediating Mg 2+ uptake in the sub-millimolar range of Mg 2+ . The AtMGT9 gene was expressed most strongly in mature anthers and was also detectable in vascular tissues of the leaves, and in young roots. Disruption of AtMGT9 gene expression resulted in abortion of half of the mature pollen grains in heterozygous mutant +/mgt9, and no homozygous mutant plant was obtained in the progeny of selfed +/mgt9 plants. Transgenic plants expressing AtMGT9 in these heterozygous plants can recover the pollen phenotype to the wild type. In addition, At-MGT9 RNAi transgenic plants also showed similar abortive pollen phenotype to mutant +/mgt9. Together, our results demonstrate that AtMGT9 functions as a low-affinity Mg 2+ transporter that plays a crucial role in male gametophyte development and male fertility.

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Section: Discussionmentioning

confidence: 99%

Magnesium transporter AtMGT9 is essential for pollen development in Arabidopsis

Chen

Liu

et al. 2009

Cell Res

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“…In Arabidopsis, like in most crop species, the genomes of cytoplasmic organelles are inherited uniparentally from the mother via the egg cell. Despite the importance of maternal cytoplasm in breeding [28][29][30] , its effect on phenotype is not easy to ascertain because producing different nuclear versus cytoplasmic combinations normally requires hybridization followed by multiple generations of backcrossing. Genome elimination during haploid induction provides a rapid method for producing cytoplasm-swapped genotypes because the maternal cytoplasm is maintained while the entire maternal nuclear HI genome is eliminated 16 .…”

Section: Resultsmentioning

confidence: 99%

A haploid genetics toolbox for Arabidopsis thaliana

et al. 2014

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Genetic analysis in haploids provides unconventional yet powerful advantages not available in diploid organisms. In Arabidopsis thaliana, haploids can be generated through seeds by crossing a wild-type strain to a transgenic strain with altered centromeres. Here we report the development of an improved haploid inducer (HI) strain, SeedGFP-HI, that aids selection of haploid seeds prior to germination. We also show that haploids can be used as a tool to accelerate a variety of genetic analyses, specifically pyramiding multiple mutant combinations, forward mutagenesis screens, scaling down a tetraploid to lower ploidy levels and swapping of nuclear and cytoplasmic genomes. Furthermore, the A. thaliana HI can be used to produce haploids from a related species A. suecica and generate homozygous mutant plants from strong maternal gametophyte lethal alleles, which is not possible via conventional diploid genetics. Taken together, our results demonstrate the utility and power of haploid genetics in A. thaliana.

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“…The isolation of Rf2a, a maize nuclear restorer gene of cytoplasmic male sterility, revealed that it encodes a mitochondrial class-2 ALDH (Cui et al 1996). RF2a has been demonstrated to be involved in anther development and plays a critical role in producing functional male gametes (Liu et al 2001;Liu and Schnable 2002).…”

Section: Introductionmentioning

confidence: 99%

Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde dehydrogenase gene from maize (Zea mays)

Huang

Wang

et al. 2008

Plant Mol Biol

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Aldehyde dehydrogenases (ALDHs) play a central role in detoxification processes of aldehydes generated in plants when exposed to the stressed conditions. In order to identify genes required for the stresses responses in the grass crop Zea mays, an ALDH (ZmALDH22A1) gene was isolated and characterized. ZmALDH22A1 belongs to the family ALDH22 that is currently known only in plants. The ZmALDH22A1 encodes a protein of 593 amino acids that shares high identity with the orthologs from Saccharum officinarum (95%), Oryza sativa (89%), Triticum aestivum (87%) and Arabidopsis thaliana (77%), respectively. Real-time PCR analysis indicates that ZmALDH22A1 is expressed differentially in different tissues. Various elevated levels of ZmALDH22A1 expression have been detected when the seedling roots exposed to abiotic stresses including dehydration, high salinity and abscisic acid (ABA). Tomato stable transformation of construct expressing the ZmALDH22A1 signal peptide fused with yellow fluorescent protein (YFP) driven by the CaMV35S-promoter reveals that the fusion protein is targeted to plastid. Transgenic tobacco plants overexpressing ZmALDH22A1 shows elevated stresses tolerance. Stresses tolerance in transgenic plants is accompanied by a reduction of malondialdehyde (MDA) derived from cellular lipid peroxidation.

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The rf2 Nuclear Restorer Gene of Male-Sterile T-Cytoplasm Maize

Cited by 360 publications

References 34 publications

Magnesium transporter AtMGT9 is essential for pollen development in Arabidopsis

Magnesium transporter AtMGT9 is essential for pollen development in Arabidopsis

A haploid genetics toolbox for Arabidopsis thaliana

Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde dehydrogenase gene from maize (Zea mays)

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