“…The PML protein appears to be the primary scaffold for these bodies (Ishov et al, 1999). PML can self-assemble via elements within its Tripartite Motif (TRIM) (Antolini et al, 2003; Huang et al, 2014) and also via binding of its conserved SIM element to SUMOs conjugated at up to eight sites in the protein (Nisole et al, 2013; Shen et al, 2006). Though not strictly required for body assembly (Brand et al, 2010; Sahin et al, 2014), SUMO-SIM interactions likely contribute substantially to body architecture, as deletion of the SIM motif or perturbations to PML SUMOylation via mutagenesis, viral infection, or knockdown/overexpression of SUMO ligases/proteases can cause changes in the size, number, morphology, or dynamics of PML NBs (Best et al, 2002; Hattersley et al, 2011; He et al, 2015; Müller and Dejean, 1999; Shen et al, 2006; Weidtkamp-Peters et al, 2008).…”