The axonal guidance factor netrin-1 as a potential modulator of swine follicular function

Basini, Giuseppina; Cortimiglia, Claudia; Baioni, Laura; Bussolati, Simona; Grolli, Stefano; Ramoni, Roberto; Grasselli, Francesca

doi:10.1016/j.mce.2010.08.001

Cited by 18 publications

(7 citation statements)

References 31 publications

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“…Netrin-1 also is a crucial factor for regulating angiogenesis in placenta [56] and osteoclast differentiation [57]. Basini et al [58] identified the NTN1 gene as a potential modulator of swine follicular function.…”

Section: Discussionmentioning

confidence: 99%

A genome-wide single nucleotide polymorphism and copy number variation analysis for number of piglets born alive

et al. 2019

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Background In this study we integrated the CNV (copy number variation) and WssGWAS (weighted single-step approach for genome-wide association) analyses to increase the knowledge about number of piglets born alive, an economically important reproductive trait with significant impact on production efficiency of pigs. Results A total of 3892 samples were genotyped with the Porcine SNP80 BeadChip. After quality control, a total of 57,962 high-quality SNPs from 3520 Duroc pigs were retained. The PennCNV algorithm identified 46,118 CNVs, which were aggregated by overlapping in 425 CNV regions (CNVRs) ranging from 2.5 Kb to 9718.4 Kb and covering 197 Mb (~ 7.01%) of the pig autosomal genome. The WssGWAS identified 16 genomic regions explaining more than 1% of the additive genetic variance for number of piglets born alive. The overlap between CNVR and WssGWAS analyses identified common regions on SSC2 (4.2–5.2 Mb), SSC3 (3.9–4.9 Mb), SSC12 (56.6–57.6 Mb), and SSC17 (17.3–18.3 Mb). Those regions are known for harboring important causative variants for pig reproductive traits based on their crucial functions in fertilization, development of gametes and embryos. Functional analysis by the Panther software identified 13 gene ontology biological processes significantly represented in this study such as reproduction, developmental process, cellular component organization or biogenesis, and immune system process, which plays relevant roles in swine reproductive traits. Conclusion Our research helps to improve the understanding of the genetic architecture of number of piglets born alive, given that the combination of GWAS and CNV analyses allows for a more efficient identification of the genomic regions and biological processes associated with this trait in Duroc pigs. Pig breeding programs could potentially benefit from a more accurate discovery of important genomic regions. Electronic supplementary material The online version of this article (10.1186/s12864-019-5687-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

A genome-wide single nucleotide polymorphism and copy number variation analysis for number of piglets born alive

et al. 2019

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“…Among them, three DEmRNAs, NTN1, AMIGO2, and ENSCHIG00000016389, were located near ENSCHIT00000007167, ENSCHIT00000002077, and ENSCHIT00000010661, respectively, which involved in the follicular process potentially. Study had shown that NTN1 could inhibit granulosa cell viability and estradiol 17β levels while it stimulates progesterone production (Basini et al, 2011).The potential core regulating factors, 32 target pairs of DElncRNA-DEmRNA with raw P value, were predicted using bioinformatics analysis in our study. 2 of target mRNA with negative correlation, ENSCHIT00000007977-POMP and ENSCHIT00000000834-ACTR3, were upregulated in large follicles.…”

Section: Discussionmentioning

confidence: 94%

Identification of mRNAs and lncRNAs Involved in the Regulation of Follicle Development in Goat

Zhao

Zou

et al. 2020

Front. Genet.

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Follicular development and maturation has a significant impact on goat reproductive performance, and it is therefore important to understand the molecular basis of this process. The importance of long non-coding RNAs (lncRNAs) in mammalian reproduction has been established, but little is known about the roles of lncRNAs in different follicular stages, especially in goats. In this study, RNA sequencing (RNA-seq) of large follicles (>10 mm) and small follicles (<3 mm) of Chuanzhong black goats was performed to investigate the regulatory mechanisms of lncRNAs and mRNAs in follicular development and maturation. A total of 8 differentially expressed lncRNAs (DElncRNAs) and 1,799 DEmRNAs were identified, and the majority of these were upregulated in small follicles. MRO, TC2N, CDO1, and NTRK1 were potentially associated with follicular maturation. KEGG pathway analysis showed that the DEmRNAs involved in ovarian steroidogenesis (BMP6, CYP11A1, CYP19A1, 3BHSD, STAR, LHCGR, and CYP51A1) and cAMP signaling play roles in regulating follicular maturation and developmental inhibition respectively. Five target pairs of DElncRNA-DEmRNA, namely, ENSCHIT00000001255-OTX2, ENSCHIT00000006005-PEG3, ENSCHIT00000009455-PIWIL3, ENSCHIT00000007977-POMP, and ENSCHIT00000000834-ACTR3 in co-expression analysis provide a clue in follicular development and maturation of lncRNA-mRNA interaction. Our findings provide a valuable resource for lncRNA studies, and could potentially provide a deeper understanding of the genetic basis and molecular mechanisms of goat follicular development and maturation.

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“…A similar geneactivation effect might occur ectopically on granulosa cell tumor cells, or perhaps it would imply some aspects of neuronal differentiation of granulosa cells. An axonal guidance factor or related gene sets were reported to be involved in granulosa cell function 39,40 , and the axon guidance was actually one of up-regulated gene sets with PS treatment in KEGG pathways, as above described. Taken together, GSEA revealed that PS treatment suppressed DNA replication-or cell cycle-related gene expression which led to chemotherapeutic cell death and in addition, induced activation of adherens junction towards a normalized direction as well as activation of neuron-related gene sets that might imply unexpected differentiation potential due to epigenetic modi cation by a HDAC inhibitor.…”

Section: Discussionmentioning

confidence: 77%

Histone Deacetylase Inhibitor, Panobinostat Exerts Anti-proliferative Effect with Partial Normalization from Aberrant Epigenetic States on Granulosa Cell Tumor Cell Lines

Hazama

Tsujioka

Sakamoto

et al. 2021

Preprint

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The prognosis of the patients with inoperable or advanced granulosa cell tumors (GCTs) is still poor, and therefore it is important to establish a novel treatment strategy. Here we investigated the in vitro effects of a histone deacetylase inhibitor, panobinostat (PS) on two GCT cell lines (KGN and COV434). GCT cell lines were found to be susceptible to PS treatment and it inhibited cell growth mainly by apoptosis. In cell cycle analysis, PS reduced only the ratio of S phase in GCT cell lines. Combined treatment of PS with a deubiquitinases inhibitor, VLX1570 exerted an additive anti-proliferative effect on KGN and COV434. The gene set enrichment analysis revealed that PS treatment suppressed DNA replication- or cell cycle-related gene expression which led to chemotherapeutic cell death and in addition, this treatment induced activation of the gene set of adherens junction towards a normalized direction as well as activation of neuron-related gene sets that might imply unexpected differentiation potential due to epigenetic modification by a HDAC inhibitor in KGN cells. In the present study, we indicate a basis of a novel therapeutic availability of a HDAC inhibitor for the treatment of GCTs and further investigations will be warranted.

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The axonal guidance factor netrin-1 as a potential modulator of swine follicular function

Cited by 18 publications

References 31 publications

A genome-wide single nucleotide polymorphism and copy number variation analysis for number of piglets born alive

A genome-wide single nucleotide polymorphism and copy number variation analysis for number of piglets born alive

Identification of mRNAs and lncRNAs Involved in the Regulation of Follicle Development in Goat

Histone Deacetylase Inhibitor, Panobinostat Exerts Anti-proliferative Effect with Partial Normalization from Aberrant Epigenetic States on Granulosa Cell Tumor Cell Lines

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