The avian embryo to study development of the cardiac conduction system

Kelder, Tim P.; Vicente‐Steijn, Rebecca; Poelmann, Robert E.; Mummery, Christine L.; DeRuiter, Marco C.; Jongbloed, Monique R.M.

doi:10.1016/j.diff.2016.01.006

Cited by 8 publications

(9 citation statements)

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“…To perform microinjection of minute quantities of either vital dye or viral constructs, the embryo needs to be physically accessible to the researcher. For this reason most labelling experiments examining heart development using micro-injections have been performed in oviparous vertebrates, such as zebrafish [45], xenopus [46] and chick embryos [47,48]. For use in mice, it is first necessary to extract the embryo from the uterus, followed by re-incubation in culture medium, for example in rolling bottles [49].…”

Section: Limitations Of Vital Dye and Viral Labellingmentioning

confidence: 99%

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The Role of Cell Tracing and Fate Mapping Experiments in Cardiac Outflow Tract Development, New Opportunities through Emerging Technologies

Peterson

Kelder

Goumans

et al. 2021

JCDD

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Whilst knowledge regarding the pathophysiology of congenital heart disease (CHDs) has advanced greatly in recent years, the underlying developmental processes affecting the cardiac outflow tract (OFT) such as bicuspid aortic valve, tetralogy of Fallot and transposition of the great arteries remain poorly understood. Common among CHDs affecting the OFT, is a large variation in disease phenotypes. Even though the different cell lineages contributing to OFT development have been studied for many decades, it remains challenging to relate cell lineage dynamics to the morphologic variation observed in OFT pathologies. We postulate that the variation observed in cellular contribution in these congenital heart diseases might be related to underlying cell lineage dynamics of which little is known. We believe this gap in knowledge is mainly the result of technical limitations in experimental methods used for cell lineage analysis. The aim of this review is to provide an overview of historical fate mapping and cell tracing techniques used to study OFT development and introduce emerging technologies which provide new opportunities that will aid our understanding of the cellular dynamics underlying OFT pathology.

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Section: Limitations Of Vital Dye and Viral Labellingmentioning

confidence: 99%

“…1: somite 1, 2: somite 2, OV: otic vesicle. Image obtained with permission from TP Kelder & R Vincente-Steijn et al [ 48 ].…”

Section: Figurementioning

confidence: 99%

The Role of Cell Tracing and Fate Mapping Experiments in Cardiac Outflow Tract Development, New Opportunities through Emerging Technologies

Peterson

Kelder

Goumans

et al. 2021

JCDD

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“…The chick embryo is readily accessible for microsurgical procedures and follow-up after intervention is relatively easy (Kelder et al, 2016). Inhibition of RHOA-ROCK signaling was achieved as previously described (Vicente-Steijn et al, 2017).…”

Section: In Ovo Rock Inhibitionmentioning

confidence: 99%

Disruption of RHOA‐ROCK Signaling Results in Atrioventricular Block and Disturbed Development of the Putative Atrioventricular Node

Kelder

Vicente‐Steijn

Poelmann

et al. 2018

The Anatomical Record

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The RHOA‐ROCK signaling pathway is involved in numerous developmental processes, including cell proliferation, differentiation and migration. RHOA is expressed in the atrioventricular node (AVN) and altered expression of RHOA results in atrioventricular (AV) conduction disorders in mice. The current study aims to detect functional AVN disorders after disturbing RHOA‐ROCK signaling in chicken embryos. RHOA‐ROCK signaling was inhibited chemically by using the Rho‐kinase inhibitor compound Y‐27632 in avian embryos (20 experimental and 29 control embryos). Morphological examination of control embryos show a myocardial sinus venosus to atrioventricular canal continuity, contributing to the transitional zone of the AVN. ROCK inhibited embryos revealed lateralization and diminished myocardial sinus venosus to atrioventricular canal continuity and at the severe end of the phenotype hypoplasia of the AVN region. Ex ovo micro‐electrode recordings showed an AV conduction delay in all treated embryos as well as cases with first, second (Wenkebach and Mobitz type) and third‐degree AV block which could be explained by the spectrum of severity of the morphological phenotype. Laser capture microdissection and subsequent qPCR of tissue collected from this region revealed disturbed expression of HCN1, ISL1, and SHOX2. We conclude that RHOA‐ROCK signaling is essential for normal morphological development of the myocardial continuity between the sinus venosus and AVN, contributing to the transitional zone, and possibly the compact AVN region. Disturbing the RHOA‐ROCK signaling pathway results in AV conduction disturbances including AV block. The RHOA‐ROCK inhibition model can be used to further study the pathophysiology and therapeutic strategies for AV block. Anat Rec, 302:83–92, 2019. © 2018 Wiley Periodicals, Inc.

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“…The advantages of using embryonic chicks have been reviewed by [8,9]. Briefly, they are easy to access since they develop ex utero.…”

Section: Introductionmentioning

confidence: 99%

Mechanosensitive Pathways in Heart Development: Findings from Chick Embryo Studies

Alser

Shurbaji

Yalcin

2021

JCDD

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The heart is the first organ that starts to function in a developing embryo. It continues to undergo dramatic morphological changes while pumping blood to the rest of the body. Genetic regulation of heart development is partly governed by hemodynamics. Chick embryo is a major animal model that has been used extensively in cardiogenesis research. To reveal mechanosensitive pathways, a variety of surgical interferences and chemical treatments can be applied to the chick embryo to manipulate the blood flow. Such manipulations alter expressions of mechanosensitive genes which may anticipate induction of morphological changes in the developing heart. This paper aims to present different approaches for generating clinically relevant disturbed hemodynamics conditions using this embryonic chick model and to summarize identified mechanosensitive genes using the model, providing insights into embryonic origins of congenital heart defects.

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The avian embryo to study development of the cardiac conduction system

Cited by 8 publications

References 106 publications

The Role of Cell Tracing and Fate Mapping Experiments in Cardiac Outflow Tract Development, New Opportunities through Emerging Technologies

The Role of Cell Tracing and Fate Mapping Experiments in Cardiac Outflow Tract Development, New Opportunities through Emerging Technologies

Disruption of RHOA‐ROCK Signaling Results in Atrioventricular Block and Disturbed Development of the Putative Atrioventricular Node

Mechanosensitive Pathways in Heart Development: Findings from Chick Embryo Studies

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