The Avian Embryo as a Model System for Skeletal Myogenesis

Hirst, Claire Elizabeth; Marcelle, Christophe

doi:10.1007/978-3-662-44608-9_5

Cited by 19 publications

(16 citation statements)

References 141 publications

(141 reference statements)

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“…The use of siRNA for downregulating gene expression have been reported in chick embryos in vivo [8], but our work is one of the first reports of the use of siRNA knockdown experiments in chick muscle cells grown in vitro. We suggest that this technique could be a powerful tool for functional analysis of sequence-specific gene silencing during chick myogenesis.…”

Section: Discussionmentioning

confidence: 98%

Knockdown of Lmo7 inhibits chick myogenesis

et al. 2016

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Edited by Dietmar MansteinThe multifunctional protein Lmo7 has been implicated in some aspects of myogenesis in mammals. Here we studied the distribution and expression of Lmo7 and the effects of Lmo7 knockdown in primary cultures of chick skeletal muscle cells. Lmo7 was localized within the nuclei of myoblasts and at the perinuclear region of myotubes. Knockdown of Lmo7 using siRNA specific to chick reduces the number and width of myotubes and the number of MyoD positive-myoblasts. Both Wnt3a enriched medium and Bio, activators of the Wnt/beta-catenin pathway, could rescue the effects of the Lmo7 knockdown suggesting a crosstalk between the Wnt/beta-catenin and Lmo7-mediated signaling pathways. Our data shows a role of Lmo7 during the initial events of chick skeletal myogenesis, particularly in myoblast survival.

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Section: Discussionmentioning

confidence: 98%

Knockdown of Lmo7 inhibits chick myogenesis

et al. 2016

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“…Myogenic proteins are highly conserved among mammalian and avian embryos [26] and many major discoveries in muscle development depend on avian model systems, especially the chick [27] . The chicken is the first model organism to be used and is excellent for developmental investigations [28,29] .…”

Section: Introductionmentioning

confidence: 99%

The genetic regulation of skeletal muscle development: insights from chicken studies

Luo¹,

Abdalla²,

Nie³

et al. 2017

Front. Agr. Sci. Eng.

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Skeletal muscle development is a complex multi-process trait regulated by various genetic factors. The chicken embryo is an ideal model system for studying skeletal muscle development. However, only a small proportion of the genetic factors affecting skeletal muscle development have been identified in chicken. The aim of this review is to summarize recent knowledge about the genetic factors involved in the regulation of skeletal muscle development in the chicken, such as gene polymorphisms, epigenetic modification, noncoding RNAs and transcription factors, which can influence skeletal muscle development at the genome, epigenome, transcriptome and proteome levels. Research on the regulation of skeletal muscle development in chicken is not yet comprehensive and most of the candidate genes and single nucleotide polymorphisms related to chicken muscle growth remain to be verified in experimental studies. In addition, the data derived from transcriptome sequencing and genome-wide association studies still require further investigation and analysis and comprehensive studies on the regulation of chicken skeletal muscle development will continue as a major research focus.

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“…Chicken embryos in particular have been used to elucidate the molecular mechanisms underpinning the morphogenesis of many systems (Guillot and Lecuit 2013;Le Douarin and Dieterlen-Lievre 2013;Hirst and Marcelle 2015;Hu et al 2015). The popularity of chicken egg-based models is due to their availability, short 21-d incubation period, and relative ease of manipulation compared to mammalian embryos.…”

Section: Introductionmentioning

confidence: 99%

Validating a Noninvasive Technique for Monitoring Embryo Movement In Ovo

Pollard

Pitsillides

Portugal

2016

Physiological and Biochemical Zoology

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Avian embryos are a commonly used model system for developmental studies, but monitoring of physiological parameters such as heart rate (HR) and movement in ovo poses a challenge to researchers. These are also increasingly common research objectives for ecological and embryo behavior studies in oviparous species. We therefore explored the validity of a new digital egg-monitoring system for the noninvasive monitoring of these parameters. We tested the relationship between frequency-ofmovement values gathered by digital monitoring and those gathered by the current standard method, which is comparatively invasive and requires egg windowing, and demonstrated that the digital monitoring method effectively distinguishes individual movements but cannot reliably monitor HR in actively motile embryos. We therefore provide recommendations for the appropriate use of this technique for avian physiologists. We also applied the digital monitoring method to reveal how frequency of movement varies throughout prenatal ontogeny in the chicken and showed that commonly used protocols in developmental studies can themselves alter motility; egg windowing and application of light modulate frequency of movement. Recent work has revealed the importance of embryo motility in regulating gene expression and cellular activity during developmental processes. Together with our data, this highlights the value of noninvasive monitoring methods and the importance of controlling for altered embryo motility/behavior in developmental studies.

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The Avian Embryo as a Model System for Skeletal Myogenesis

Cited by 19 publications

References 141 publications

Knockdown of Lmo7 inhibits chick myogenesis

Knockdown of Lmo7 inhibits chick myogenesis

The genetic regulation of skeletal muscle development: insights from chicken studies

Validating a Noninvasive Technique for Monitoring Embryo Movement In Ovo

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