1986
DOI: 10.1073/pnas.83.18.6795
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The avian beta-adrenergic receptor: primary structure and membrane topology.

Abstract: Partial amino acid sequence information allowed the isolation of cDNA clones encoding the turkey erythrocyte (3-adrenergic receptor. Antisera raised against synthetic peptides encoded by the cDNA crossreacted with the purified receptor and appropriate tryptic fragments, confirming the identity of the cDNA. The receptor is composed of 483 amino acids and has a molecular mass of 54 kDa. Its sequence suggests that it is arranged predominantly in seven membranespanning sequences and a long cytoplasmic carboxyl-ter… Show more

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Cited by 223 publications
(57 citation statements)
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References 35 publications
(34 reference statements)
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“…Incubation of the affinity-labeled 50 kDa receptor with endoglycosidase F resulted in a change in its electrophoretic migration corresponding to a molecular mass of 45 kDa (not shown), as described before [12], indicating that the carbohydrate moiety located in the N-terminal region [2,7] of the affinity-labeled t45'AR was removed by the glycosidase. Upon treatment of the NTCBlabeled fragments (FI8, F23, F28) with endoglycosidase F, an electrophoretic pattern identical to that of the untreated fragments ( fig.…”
Section: Enzymatic Treatments Of Ntcb-labeled Fragmentssupporting
confidence: 57%
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“…Incubation of the affinity-labeled 50 kDa receptor with endoglycosidase F resulted in a change in its electrophoretic migration corresponding to a molecular mass of 45 kDa (not shown), as described before [12], indicating that the carbohydrate moiety located in the N-terminal region [2,7] of the affinity-labeled t45'AR was removed by the glycosidase. Upon treatment of the NTCBlabeled fragments (FI8, F23, F28) with endoglycosidase F, an electrophoretic pattern identical to that of the untreated fragments ( fig.…”
Section: Enzymatic Treatments Of Ntcb-labeled Fragmentssupporting
confidence: 57%
“…residues located along the polypeptide chain [2]; indeed, the smallest labeled polypeptide observed after cleavage was still twice the size (15 kDa) of the longest segment between two neighbouring cysteine residues present in the protein (Cysl°-CysSS).…”
Section: Localization Of the Labeled Fragments In The Receptor Polypementioning
confidence: 96%
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