The Autoimmune Antigen me is Distinct and Related to Undifferentiated Connective Tissue Disease

Treadwell, Edward L.; Boak, Andra M.; Kovacs, Shirley A. H.; Chen, Jintao; Wang, Richard J.; Sharp, Gordon C.; Agris, Paul F.

doi:10.1002/art.1780301106

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Establishment Of Human Antibody-producing Lines1

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1988

2004

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Cited by 5 publications

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“…Immunoblotting. Immunoblotting was performed as previously described (34), except that purified (U1)snRNP was loaded onto 13% SDS-polyacrylamide gels at 0.012 mg proteinkm of gel slot.…”

Section: Establishment Of Human Antibody-producing Linesmentioning

confidence: 99%

Human autoantibody secreted by immortalized lymphocyte cell line against the 68k polypeptide of the u1 small nuclear ribonucleoprotein

Chen

Takeda

Vanderslice

et al. 1988

Arthritis & Rheumatism

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Autoantibody of an immortalized human lymphocyte cell line, Su-2E4, derived from peripheral lymphocytes of a patient with mixed connective tissue disease, showed specific binding of the 68K polypeptide of U1 small nuclear RNP (snRNP) and immunoprecipitation of U1 RNA. The reaction patterns of Su-2E4 and a murine monoclonal anti-(U1)snRNP line, 2.73, and results of a competition assay with the 2 antibodies suggest similar, but not necessarily identical, epitope recognition.Autoantibodies to nuclear and cytoplasmic components are frequently found in patients with systemic rheumatic diseases (1-3). The autoantigens are often nucleic acid-associated proteins, such as small nuclear RNP (snRNP) (4). Circulating autoantibodies to some of these antigens are characteristic of Presented by Jintao Chen in partial fulfillment of the requirements for a PhD degree,

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“…Immunoblotting. Immunoblotting was performed as previously described (34), except that purified (U1)snRNP was loaded onto 13% SDS-polyacrylamide gels at 0.012 mg proteinkm of gel slot.…”

Section: Establishment Of Human Antibody-producing Linesmentioning

confidence: 99%