The autofluorescence of plastic materials and chips measured under laser irradiation

Piruska, Aigars; Nikčević, Irena; Lee, Se Hwan; Ahn, Chong H.; Heineman, William R.; Limbach, Patrick A.; Seliskar, Carl J.

doi:10.1039/b508288a

Cited by 362 publications

(274 citation statements)

References 6 publications

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“…A negative side of Mylar is it has relatively high intensity of background fluorescence if excited in ultraviolet, blue, and green regions (Hawkins and Yager 2003;Piruska et al 2005). Meanwhile, with the optical configuration used in this experiment, the background fluorescence of the microfluidic substrates, including from the bottom and the top covers of microchannels, will partly be collected and transmitted to detector.…”

Section: Resultsmentioning

confidence: 99%

Modeling Fluorescence Detection for Microfluidic Card

Irawan

Tjin

Zhang

et al. 2007

International Journal of Optomechatronics

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Simple fluorescence detection system and disposable microfluidic card have been developed and tested. A blue LED, optical fiber, CCD detector, Mylar and PMMA, and fluorescein and BSA-FITC were used as an excitation source, waveguide, detector, microfluidic substrate, and sample, respectively. The results show the system can detect 0.01-1000 ng/ml of fluorescein solutions with linear response. A technique to avoid cross-talks between the adjacent channels in a multi-channel microfluidic card is also addressed. The test using BSA as a model analyte demonstrates its feasibility for on-chip biosensor applications. This prototype can be used as a platform to develop a compact bio-fluorescence sensor for microfluidic analysis systems.

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Section: Resultsmentioning

confidence: 99%

Modeling Fluorescence Detection for Microfluidic Card

Irawan

Tjin

Zhang

et al. 2007

International Journal of Optomechatronics

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“…The data compare well with values reported for other relevant material such as COC and PMMA. [32] The bonding quality was investigated using fluorescence imaging with Rhodamine B, as shown in Figure 4. The chip was incubated for 69 h and neither creeping of the fluorescent dye at the bond interface, nor diffusion of the dye inside the polymer could be observed.…”

Section: Resultsmentioning

confidence: 99%

Modeling and simulation of electrostatically gated nanochannels

Pardon

Wijngaart

2013

Advances in Colloid and Interface Science

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A new method that allows for mold-free, rapid and easy-to-use prototyping of microfluidic devices comprising channels, access holes and surface modified patterns, is presented. The innovative method is based on direct photolithographic patterning of an off-stoichiometry thiol-ene (OSTE) polymer formulation, tailor-made for photolithography, which offers unprecedented spatial resolution and allows for efficient, robust and reliable, room temperature surface modification and glue-free, covalent room temperature bonding. This mold-free process does not require cleanroom equipment and therefore allows for rapid, i.e. less than one hour, design-fabricate-test cycles, using a material suited for larger scale production. The excellent photolithographic properties of this new OSTE formulation allow for unprecedented, for thiol-ene polymer systems, resolution patterning in hundreds of micrometers thick layers, 200 µm thick in this work. Moreover, we demonstrated robust (covalent) and spatially-controlled modification of the microchannel surfaces with a contact angle of 76 degrees to hydrophobic/hydrophilic areas with contact angles of 102 and 43 degrees, respectively.

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“…Cells grow attached to the bottom surface of the chamber. To minimize autofluorescence of plastic packaging material, PMMA has been used as a top cover of lCCA (16).…”

Section: Original Articlementioning

confidence: 99%

Real‐time fluorescence detection of multiple microscale cell culture analog devices in situ

Sung

Tatosian

et al. 2007

Cytometry Pt A

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We investigated multiple microscale cell culture analog (lCCA) assays in situ with a high-throughput imaging system that provides quantitative, nondestructive, and realtime data on cell viability. Since samples do not move between measurements, captured images allow accurate time-course measurements of cell population response and tracking the fate of each cell type on a quantitative basis. The optical system was evaluated by measuring the short-term response to ethanol exposure and long-term growth of drug-resistant tumor cell lines with simultaneous samples. The optical system based on epi-fluorescent excitation consists of an LED and a CCD as well as discrete optical components for imaging a large number of cells simultaneously. HepG2/C3A and MESSA cell lines were cultured in two lCCA systems for continuous cell status monitoring in cell death experiments with ethanol and long-term cell growth. The experiment that tested ethanol uptake showed that ethanol immediately caused cell death. The system was applied to extracting dynamic constants in the uptake process. In the long-term cell growth experiment, growth of MESSA cells was followed by a stationary phase and eventual cell death attributed to nutrient and oxygen depletion and a change in the pH because of the accumulation of wastes by cell metabolism. HepG2/C3A cells were subject to contact inhibition and cell number did not change significantly over time. Issues related to long-term assays are also discussed. The quantitative results have been consistent with qualitative images and confirm the applicability of the portable optical system, and potential application to high-throughput analysis of cell-based assays to measure long-term dynamics. ' International Society for Analytical CytologyKey terms fluorescence detection; high-throughput screening; microscale cell culture analog; live cell imaging; cell status monitoring; green-fluorescent protein; cell dynamics A cell culture analog (CCA) device is a cell-based assay using interconnected compartments with different cell type and can be used to evaluate toxicity and efficacy of various pharmacological agents (1). Using the well-established semiconductor microfabrication technology, a microscale cell culture analog (lCCA) is fabricated from a silicon wafer. It consists of separate chambers for representing key organs in the human body, which are connected by microfluidic channels. The sizes of chambers and channels are designed so that they give similar residence times and liquid-to-cell ratios to the physiological values in the human body. A lCCA system has an advantage that it can mimic animal tissue dimensions more realistically than larger tissue culture vessels. This technology has been previously shown to replicate toxicological events that are undetectable using ordinary in vitro experiments (2,3). Microfluidic-based cell chips such as the lCCA presented in this paper are expected to provide a tool to decrease the time and cost of the drug discovery process especially in the phase prior to...

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The autofluorescence of plastic materials and chips measured under laser irradiation

Cited by 362 publications

References 6 publications

Modeling Fluorescence Detection for Microfluidic Card

Modeling Fluorescence Detection for Microfluidic Card

Modeling and simulation of electrostatically gated nanochannels

Real‐time fluorescence detection of multiple microscale cell culture analog devices in situ

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