The autoantigen La/SSB is a calmodulinmbinding protein

Castro, Anna; Faura, M.; Agell, Neus; Renau-Plqueras, J.; Bachs, Oriol

doi:10.1016/s0143-4160(96)90091-3

Cited by 11 publications

(7 citation statements)

References 38 publications

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“…Calmodulin and calreticulin are calcium binding proteins which mediate the biological effects of Ca 2+ [56]. These proteins were found to be up-regulated in salivary gland cells of SS patients which might be due to the interaction with autoantigen La/SSB [25], [26]. The over-expression of calreticulin, in fact, caused a decrease in the rate of Ca 2+ mobilization from the internal stores [57].…”

Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Protein and Gene Expression in Salivary Glands of Sjogren’s-Like Disease NOD Mice Treated by Bone Marrow Soup

et al. 2014

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BackgroundBone marrow cell extract (termed as BM Soup) has been demonstrated to repair irradiated salivary glands (SGs) and restore saliva secretion in our previous study. In the present study, we aim to investigate if the function of damaged SGs in non-obese diabetic (NOD) mice can be restored by BM Soup treatment and the molecular alterations associated with the treatment.MethodsWhole BM cells were lysed and soluble intracellular contents (“BM Soup”) were injected I.V. into NOD mice. Tandem mass tagging with 2-D liquid chromatography-mass spectrometry was used to quantify proteins in the submandibular glands (SMGs) between untreated and BM Soup-treated mice. Quantitative PCR was used to identify genes with altered expression in the treated mice.Results BM Souprestored salivary flow rates to normal levels and significantly reduced the focus scores of SMGs in NOD mice. More than 1800 proteins in SMG cells were quantified by the proteomic approach. Many SMG proteins involved in inflammation and apoptosis were found to be down-regulated whereas those involved in salivary gland biology and development/regeneration were up-regulated in the BM Soup-treated mice. qPCR analysis also revealed expression changes of growth factors and cytokines in the SMGs of the treated NOD mice.ConclusionBM Soup treatment is effective to restore the function of damaged SGs in NOD mice. Through gene/protein expression analysis, we have found that BM Soup treatment might effectuate via inhibiting apoptosis, focal adhesion and inflammation whereas promoting development, regeneration and differentiation of the SG cells in NOD mice. These findings provide important insights on the potential mechanisms underlying the BM Soup treatment for functional restoration of damaged SGs in NOD mice. Additional studies are needed to further confirm the identified target genes and their related signaling pathways that are responsible for the BM Soup treatment.

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Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Protein and Gene Expression in Salivary Glands of Sjogren’s-Like Disease NOD Mice Treated by Bone Marrow Soup

et al. 2014

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“…Finally, the identities of the nuclear CaM-binding proteins responsible for retaining Ca 2ϩ -CaM in the nucleus remain to be determined. Possible candidates include CaM Kinase IV, a splice variant of CaM Kinase II, calcineurin, and several less completely characterized proteins, all of which exhibit nuclear localization in some cell types (30)(31)(32)(33)(34)(35). …”

Section: Discussionmentioning

confidence: 99%

Mechanism of Ca ²⁺ -dependent nuclear accumulation of calmodulin

Liao

Paschal

Luby-Phelps

1999

Proc. Natl. Acad. Sci. U.S.A.

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“…Previously, elevated levels of calmodulin‐binding proteins in the salivary glands from pSS patients have been reported (Deming et al , 2007). In particular, calmodulin is found to interact with Sjögren’s syndrome autoantigen La/SSB (Castro et al , 1996), which can explain the elevated levels of calmodulin in the minor salivary glands of pSS patients.…”

Section: Discussionmentioning

confidence: 99%