The Autoantibody Repertoire Against Copper- or Macrophage-Modified LDL Differs in Normolipidemics and Hypercholesterolemic Patients

Fernvik, Eva; Ketelhuth, Daniel F.J.; Russo, Marco; Gidlund, Magnus

doi:10.1023/b:joci.0000019782.67993.0b

Cited by 38 publications

(32 citation statements)

References 31 publications

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“…20 The samples were oxidized sequentially from 10 to 90 min, in steps of 10 min. At each predefined time, the oxidation reaction was inhibited with the addition of 1 mM of EDTA to the sample solution.…”

Section: Ldl In Vitro Oxidation By Copper Ionsmentioning

confidence: 99%

Behavior of the thermal diffusivity of native and oxidized human low-density lipoprotein solutions studied by the Z-scan technique

et al. 2012

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2012Behavior of the thermal diffusivity of native and oxidized human low-density lipoprotein solutions studied by the Z-scan technique JOURNAL OF BIOMEDICAL OPTICS, BELLINGHAM, v. 17, n. 10, supl. Abstract. Modifications in low-density lipoprotein (LDL) have emerged as a major pathogenic factor of atherosclerosis, which is the main cause of morbidity and mortality in the western world. Measurements of the heat diffusivity of human LDL solutions in their native and in vitro oxidized states are presented by using the Z-Scan (ZS) technique. Other complementary techniques were used to obtain the physical parameters necessary to interpret the optical results, e.g., pycnometry, refractometry, calorimetry, and spectrophotometry, and to understand the oxidation phase of LDL particles. To determine the sample's thermal diffusivity using the thermal lens model, an iterative one-parameter fitting method is proposed which takes into account several characteristic ZS timedependent and the position-dependent transmittance measurements. Results show that the thermal diffusivity increases as a function of the LDL oxidation degree, which can be explained by the increase of the hydroperoxides production due to the oxidation process. The oxidation products go from one LDL to another, disseminating the oxidation process and caring the heat across the sample. This phenomenon leads to a quick thermal homogenization of the sample, avoiding the formation of the thermal lens in highly oxidized LDL solutions.

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Section: Ldl In Vitro Oxidation By Copper Ionsmentioning

confidence: 99%

Behavior of the thermal diffusivity of native and oxidized human low-density lipoprotein solutions studied by the Z-scan technique

et al. 2012

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“…9,10 Immune response against oxLDL is in the heterogeneous group and can be used as an indirect marker for the presence of oxLDL in circulation. 11 Most interestingly, studies have demonstrated an association between oxLDL Ab titer and the progression of atherosclerosis. Thus oxLDL has been suggested as a marker for the progression of atherosclerosis.…”

Section: Introductionmentioning

confidence: 99%

Measurement of the nonlinear optical response of low-density lipoprotein solutions from patients with periodontitis before and after periodontal treatment: evaluation of cardiovascular risk markers

Monteiro¹,

Jardini

Giampaoli³

et al. 2012

J. Biomed. Opt

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Abstract. The Z-Scan (ZS) technique in the thermal regime has been used to measure the nonlinear optical response of low-density lipoprotein (LDL). The ZS technique is carried out in LDL from 40 patients with chronic periodontitis before and after three, six, and 12 months of periodontal treatment. Clinical parameters such as probing depths, bleeding on probing, total and differential white blood cells counts, lipid profiles, cytokine levels, and antibodies against oxidized LDL are also determined and compared over time. Before the treatment, the ZS experimental results reveal that the LDL particles of these patients are heavily modified. Only after 12 months of the periodontal treatment, the ZS results obtained reveal behavioral characteristics of healthy particles. This conclusion is also supported by complementary laboratorial analysis showing that the periodontal treatment induces systemic changes in several inflammatory markers.

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“…Modification of LDL triggers the activation of the immune system, either inducing the production of antibodies against these particles [9], activating T-cells [2] or stimulating antigen-presenting cells [10]. The oxLDL particles bound to antibodies are taken up from the circulation via Fcγ II receptors present in cells from the mononuclear phagocytic system contributing to the inflammation in the vascular wall [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Role of PPAR-gamma in the Modulation of CD36 and FcgammaRII induced by LDL with Low and High Degrees of Oxidation During the Differentiation of the Monocytic THP-1 Cell Line

Ríos¹,

Jancar²,

Melo³

et al. 2008

Cell Physiol Biochem

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Scavenger or Fcγ receptors are important for capture and clearance of modified LDL particles by monocytes/macrophages. Uptake via scavenger receptors is not regulated by intracellular levels of cholesterol and in consequence, macrophages develop into foam cells in the arterial intima. The levels of scavenger receptor CD36 are increased in atherosclerotic lesions and there is evidence that some components of oxLDL auto-regulate the expression of this receptor. Fcγ receptor expression is increased in cardiovascular diseases but it is not known weather their expression is regulated by oxLDL. The biological properties of oxLDLs vary depending on the degree of oxidation. In the present study we investigated the effect of LDL particles showing extensive or low oxidation (HoxLDL and LoxLDL) on the expression of CD36 and FcγRII in a human monocytic cell line (THP-1), differentiated or not to macrophage, and the involvement of PPARγ. It was found that both forms of oxLDL are able to increase the expression of CD36 and FcγRII and that this effect is dependent on the degree of oxidation and of the stage of cell differentiation (monocyte or macrophage). We also showed that the increased expression of FcγRII is dependent on PPARγ whereas that of the CD36 is independent of PPARγ.

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The Autoantibody Repertoire Against Copper- or Macrophage-Modified LDL Differs in Normolipidemics and Hypercholesterolemic Patients

Cited by 38 publications

References 31 publications

Behavior of the thermal diffusivity of native and oxidized human low-density lipoprotein solutions studied by the Z-scan technique

Behavior of the thermal diffusivity of native and oxidized human low-density lipoprotein solutions studied by the Z-scan technique

Measurement of the nonlinear optical response of low-density lipoprotein solutions from patients with periodontitis before and after periodontal treatment: evaluation of cardiovascular risk markers

Role of PPAR-gamma in the Modulation of CD36 and FcgammaRII induced by LDL with Low and High Degrees of Oxidation During the Differentiation of the Monocytic THP-1 Cell Line

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