The attractive recombinant phytase from Bacillus licheniformis: biochemical and molecular characterization

Abstract

The phyL gene encoding phytase from the industrial strain Bacillus licheniformis ATCC 14580 (PhyL) was cloned, sequenced, and overexpressed in Escherichia coli. Biochemical characterization demonstrated that the recombinant enzyme has an apparent molecular weight of nearly 42 kDa. Interestingly, this enzyme was optimally active at 70-75 °C and pH 6.5-7.0. This enzyme is distinguishable by the fact that it preserved more than 40 % of its activity at wide range of temperatures from 4 to 85 °C. This new phytase displayed also a high specific activity of 316 U/mg. For its maximal activity and thermostability, this biocatalyst required only 0.6 mM of Ca(2+) ion and exhibited high catalytic efficiency of 8.3 s(-1) μM(-1) towards phytic acid.

A novel phytase characterized by thermostability and high pH tolerance from rice phyllosphere isolated Bacillus subtilis B.S.46

“…KHU-10 phytase (10 mM CaCl 2 ) [30] , and recombinant phytase (rePhyCm) [34] . However, Gulati et al [17] , El-Toukhy et al [35] , Borgi et al [36] , and Nuñal et al [37] reported different findings for B. laevolacticus , B. subtilis MJA, B. licheniformis ATCC 14580 (PhyL), and different Bacillus strains, respectively. B. amyloliquefaciens US573 had a similar optimum pH of 7.5, but higher optimum temperature (70 °C) than B. subtilis B.S.46 [31] .…”

Characterization of an extremely salt-tolerant and thermostable phytase from Bacillus amyloliquefaciens US573

“…The apparent K m , V max , k cat and catalytic efficiency k cat /K m were 1.125 mM, 27.71 U/mg, 1687.37 min −1 and 1499.88 mM −1 min −1 respectively. The catalytic efficiency of PHY US573 was higher than other phytases characterized from the genus Bacillus [35][36][37] and other bacterial genera like Erwinia carotovora, Pedobacter nyackensis MJ11 CGMCC 2503, Klebsiella pneumoniae and E. coli [38,39].…”

Microbial production of phytases for combating environmental phosphate pollution and other diverse applications

“…Coban et al (2015) enhanced the phytase production in SmF by using a novel fermentation technique by testing the effect of the various microparticles on A. ficuum. Borgi et al (2014), characterized the recombinant phytase from B. licheniformis ATCC 14580 (PhyL). This enzyme (42 kDa) was optimally active at 70-75 C and pH 6.5-7.0.…”