The attenuation of chlorogenic acid on oxidative stress for renal injury in streptozotocin-induced diabetic nephropathy rats

Abstract

The present study was undertaken to investigate whether chlorogenic acid (CGA) could protect kidney function against oxidative stress in the diabetic nephropathy (DN) rats. The treatment with CGA could decrease significantly the levels of blood glucose, blood urea nitrogen and serum creatinine in DN rats. Moreover, CGA significantly increased the activity of superoxide dismutase, glutathione peroxidase, and catalase. Moreover, the level of lipid peroxidation malondialdehyde was reduced markedly after CGA administration. Immunohistochemical analysis also showed that CGA downregulated significantly cyclooxygenase-2 protein expression in renal tissue, which is considered as one of the major pathogeneses of oxidative stress. Furthermore, we demonstrated that CGA could block the expression of activating transcription factor-6, C/EBP homology protein and the phosphorylation of eukaryotic initiation factor 2α and double stranded RNA-activated protein kinase-like endoplasmic reticulum kinase. In addition, we attempted to detect the presence of diabetic renal tissues apoptosis-related proteins. Our data provided evidence to support this fact that CGA attenuated oxidative stress in streptozocin-induced DN rats. Its molecular mechanism may inhibit the endoplasmic reticulum-stress response in DN.

Nephroprotective effects of chlorogenic acid against sodium arsenite‐induced oxidative stress, inflammation, and apoptosis

“…Moreover, we found that these morphological alterations induced by As were successfully attenuated by preadministration of the well-established antioxidant, CGA. Our findings are in agreement with those reported by Ye et al 31 who demonstrated the excellent antioxidant effects of CGA against streptozotocin-induced diabetic nephropathy in rats. Moreover, Dkhil et al 4 have reported that CGA improves weight loss in animals and restores the body and liver weight to normal levels.…”

Renoprotective effect of JinQi-JiangTang tablet on high-fat diet and low-dose streptozotocin-induced type 2 diabetic rats

“…The results of our study were in line with a previous study that chlorogenic acid suppressed hyperglycemia caused oxidative damages. 40 These results indicated that JQ exhibited renoprotective effect by preventing the depletion of antioxidants, reducing lipid peroxidation and attenuating oxidative stress. Fig.…”

Cytoprotective effect of chlorogenic acid against hydrogen peroxide-induced oxidative stress in MC3T3-E1 cells through PI3K/Akt-mediated Nrf2/HO-1 signaling pathway

“…Therefore, it is tempting to suggest that exogenous administration of antioxidants should be investigated as a potential approach to protect cells against ROS for the treatment of osteoporosis. Previous studies have revealed the antioxidant effect of CGA, effectively scavenging free radicals and inhibiting lipid peroxidation [25, 30, 31]. Recent report demonstrated that CGA efficiently inhibits osteoblast apoptosis and ameliorates hormone-induced femoral head necrosis [32].…”

“…CGA, a type of polyphenolic compound formed by esterification of caffeic and quinic acids, is the major active ingredient found in many traditional Chinese medicines such as Flos Lonicerae Japonicae and Eucommia Ulmoides , and it is also abundant in some fruits, dietary vegetables, and daily beverages like coffee, pineapple, beans, strawberries and apples [23]. Previous reports have demonstrated that CGA exhibits a wide range of diverse pharmacological effects, including anti-inflammatory, anti-oxidative, and anti-carcinogenic activities [3, 24, 25]. However, the antioxidant effects and molecular mechanisms of CGA in osteoblasts have not been elucidated yet.…”