The attachment of <i>Staphylococcus epidermidis</i> on the surface of a carbon paste electrode at various positive potentials: The effect of pH, incubation time, and solid-medium type

Abstract

The attachment of <i>Staphylococcus epidermidis</i> to the surface of carbon paste electrode (CPE) by applying positive potentials (50 - 600 mV) with regard of various buffer pH, cultivation time and solid-medium type was studied. The attachment process was analyzed by measuring the electric current derived from the dye (amido black) adsorbed on the vacant areas of CPE after attachment of microbial cells. The pH was not identified as a single main factor affecting the attachment (p > 0.05), however further insight revealed that the potentials applied had different effects on the microbial cells attachment. Both cultivation time and solid-medium type significantly affected the microbial attachment. Generally, increase of cultivation time up to 168 h resulted in increase of adhesion. Applying potentials 300, 400 and 200 mV resulted in the highest attachment process for bacteria cultivated for 24, 48 and 168 h, respectively. <i>S. epidermidis</i> cultivated on the blood agar and Baird-Parker agar plates showed the higher extent of attachment

Electrochemical Control of Biofilm Formation and Approaches to Biofilm Removal

“…This phenomenon is in accordance with the previously reported data confirming an inhibition effect of alkaline media (with pH 8.5) on the adhesion of Staphylococcus epidermidis and Staphylococcus aureus cells [145]. In contrast, the adhesion of Staphylococcus epidermidis cells to the surface of a CPE was slightly enhanced in an acidic environment (pH 5) [146]. However, it should be noted that the above-mentioned knowledge cannot be used in general because a decrease in G+ Listeria monocytogenes adhesion was found in acidic conditions (pH 5) compared to a physiological pH of approximately 7 [147].…”