The atp1 and atp2 operons of the cyanobacterium Synechocystis sp. PCC 6803

Abstract

The two operons atp1 and atp2, encoding the subunits of the F0F1 ATP-synthase, have been cloned and sequenced from the cyanobacterium Synechocystis sp. PCC 6803. The organization of the different genes in the operons have been found to resemble that of the cyanobacteria Synechococcus sp. PCC 6301 and Anabaena sp. PCC 7120. The Synechocystis F0F1 ATP-synthase has nine subunits. A tenth open reading frame with unknown function was detected at the 5' end of atp1, coding for a putative gene product similar to uncI in Escherichia coli. A promoter structure was inferred for the Synechocystis atp operons and compared to other known promoters of cyanobacteria. Even though the operon structure of atp1 and atp2 in Synechocystis resembles the corresponding operons of Synechococcus, the amino acid sequences of individual gene products show marked differences. Genetic distances between cyanobacterial genes and genes for ATP-synthase subunits from other species have been calculated and compiled into evolutionary trees.

Sequence of subunit c of the Na+‐translocating F1FO ATPase of Acetobacterium woodii: proposal for determinants of Na+ specificity as revealed by sequence comparisons

“…2. Alignment of subunit c from A. woodii with subunit c from P. modestum [20], E. hirae [21], E. coli [22], V. alginolyticus [23], thermophilic bacterium PS3 [24], Bacillus megaterium [25], and Synechocystis 6803 [26]. The topology of subunit c is indicated.…”

Transcriptional Regulation of the Respiratory Genes in the CyanobacteriumSynechocystissp. PCC 6803 during the Early Response to Glucose Feeding

“…S2). In Synechocystis, the atpI/G genes form the atp1 operon together with the atpD/A/C genes (Lill and Nelson, 1991); however, accumulation of these transcripts was differentially affected by Cm treatment (Supplemental Fig. S2), indicating the possibility of either the selective regulation of transcription or differences in mRNA stability in response to Glc feeding.…”

The H+/ATP coupling ratio of the ATP synthase from thiol‐modulated chloroplasts and two cyanobacterial strains is four

“…With different techniques based on independent approaches it appears that this ratio is 4 in two membrane systems: thiol-modulated (reduced) broken spinach chloroplasts and membrane vesicles from two cyanobacterial strains, the thermophile Synechococcus 6716 and the transformable Synechocystis 6803. The F2F 0 from those cyanobacteria appears to be very closely related to CFIF 0 in sequence homology of the subunits [17,18] and functionally corresponds to the reduced form of the latter [19,201. …”