The atherosclerosis-ameliorating effects and molecular mechanisms of BuYangHuanWu decoction

Liu, Bo; Song, Zhenyan; Yu, Jingping; Li, Ping; Tang, Yuan; Ge, Jinwen

doi:10.1016/j.biopha.2019.109664

Cited by 41 publications

(24 citation statements)

References 38 publications

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“…Recent studies have reported that inflammation plays a critical role in the formation and development of AS (Lu and Kakkar, 2014). A variety of TCM compounds or monomers for activating blood have shown to inhibit the inflammatory response of AS (Kang et al, 2015;Zeng et al, 2018;Liu et al, 2020). To achieve the innovative development of TCM, there is an unmet need to improve the TCM pharmacological effects by modifying formulas with a better understanding of the underlying mechanism of TCM in the context of both host phenotypes and the gut microbiome.…”

Section: Discussionmentioning

confidence: 99%

The Enhanced Pharmacological Effects of Modified Traditional Chinese Medicine in Attenuation of Atherosclerosis Is Driven by Modulation of Gut Microbiota

et al. 2020

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Accumulating evidence indicated that gut microbiota-targeted therapy is a promising strategy to treat Cardiovascular Disease (CVD). Traditional Chinese Medicine (TCM) has been used in CVD treatments for over 2,000 years which is believed to result from the modulation of gut microbiota, yet the underlying mechanism remains elusive. According to the theoretical system of TCM, we developed an innovative formula of TCM named "TongMai ZhuYu (TMZY)" on top of one classic Chinese herbal formula ["XueFu ZhuYu (XFZY)"], which can more effectively alleviate CVD in the clinical practice. Here, we first systematically assessed the pharmacological effects of TMZY, XFZY, and atorvastatin on atherosclerosis (AS) induced by high-fat diet (HFD) in rats. TMZY typically outperformed others in alleviating AS rats by characterization of pathological morphology, immunohistochemistry, inflammatory cytokines. Remarkably, combining this modified TCM formula (TMZY) with atorvastatin can further help the alleviation of AS in rats by suppressing immune and inflammatory responses. Furthermore, to test whether TMZY alleviated AS symptoms by altering gut microbial compositions (dysbiosis), we employed 16S amplicon sequencing to investigate gut microbiota changes in the AS mice induced by high choline diet (HCD) using both TMZY and XFZY under antibiotic-treated and untreated conditions. TCM formulas induced consistent and remarkable changes in the phenotypes and microbiota in the HCD mice. TMZY modulated more changes in the gut microbiota to improve diseased phenotypes than XFZY. Notably, the TMZY-intervention effect on CVD in mice attenuated after the suppression of gut microbial activity by antibiotics. Collectively, we demonstrated that TCM herbals could effectively modulate the gut microbiota as a mechanism for altering the pathogenesis of cardiovascular disorders in mice/rats.

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Section: Discussionmentioning

confidence: 99%

The Enhanced Pharmacological Effects of Modified Traditional Chinese Medicine in Attenuation of Atherosclerosis Is Driven by Modulation of Gut Microbiota

et al. 2020

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“…Network pharmacology is a novel field that integrates pharmacology, biochemistry, genomics, and bioinformatics [ 7 ]. It is an effective strategy to visualize and analyze complex interactive data on herbs, compounds, targets, and diseases based on computer modeling analysis and target prediction methods [ 11 ] and is especially suitable for characterizing TCM formulations.…”

Section: Discussionmentioning

confidence: 99%

“…Traditional Chinese medicine (TCM) has been used to treat various illnesses for thousands of years and is associated with fewer toxic effects compared with pharmacological drugs [ 6 , 7 ]. Yisui Qinghuang powder (YSQHP), a TCM formulation used to treat MDS, consists of indigo naturalis (qingdai), red ginseng (hongshen), and realgar (xionghuang) [ 8 , 9 ], a mineral consisting of arsenic sulfide (As 4 S 4 ) and arsenic trioxide (As 2 O 3 ).…”

Section: Introductionmentioning

confidence: 99%

“…Network pharmacology integrates systems biology with polypharmacology and is a suitable approach to characterize complex TCM formulations and identify their multiple targets and mechanisms of action [ 7 , 11 ]. We identified the bioactive compounds of YSQHP using the TCMSP [ 12 ] and HerDing databases [ 13 ] and established the network of these compounds, their putative targets, and diseases.…”

Section: Introductionmentioning

confidence: 99%

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Deciphering the Key Pharmacological Pathways and Targets of Yisui Qinghuang Powder That Acts on Myelodysplastic Syndromes Using a Network Pharmacology‐Based Strategy

Han

Song²,

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Yisui Qinghuang powder (YSQHP) is an effective traditional Chinese medicinal formulation used for the treatment of myelodysplastic syndromes (MDS). However, its pharmacological mechanism of action is unclear. Materials and Methods. In this study, the active compounds of YSQHP were screened using the traditional Chinese medicine systems pharmacology (TCMSP) and HerDing databases, and the putative target genes of YSQHP were predicted using the STITCH and DrugBank databases. Then, we further screened the correlative biotargets of YSQHP and MDS. Finally, the compound-target-disease (C-T-D) network was conducted using Cytoscape, while GO and KEGG analyses were conducted using R software. Furthermore, DDI-CPI, a web molecular docking analysis tool, was used to verify potential targets and pathways. Finally, binding site analysis was performed to identify core targets using MOE software. Results. Our results identified 19 active compounds and 273 putative target genes of YSQHP. The findings of the C-T-D network revealed that Rb1, CASP3, BCL2, and MAPK3 showed the most number of interactions, whereas indirubin, tryptanthrin, G-Rg1, G-Rb1, and G-Rh2 showed the most number of potential targets. The GO analysis showed that 17 proteins were related with STPK activity, PUP ligase binding, and kinase regulator activity. The KEGG analysis showed that PI3K/AKT, apoptosis, and the p53 pathways were the main pathways involved. DDI-CPI identified the top 25 proteins related with PI3K/AKT, apoptosis, and the p53 pathways. CASP8, GSK3B, PRKCA, and VEGFR2 were identified as the correlative biotargets of DDI-CPI and PPI, and their binding sites were found to be indirubin, G-Rh2, and G-Rf. Conclusion. Taken together, our results revealed that YSQHP likely exerts its antitumor effects by binding to CASP8, GSK3B, PRKCA, and VEGFR2 and by regulating the apoptosis, p53, and PI3K/AKT pathways.

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“…As a new pharmacological discipline, integrating network pharmacology emphasizes the concept of a “multicomponent, multitarget therapeutic network” and highlights the overall concept of TCM [ 30 ]. Network pharmacology offers a novel concept for understanding the multitargeted mechanism of the treatment of complex diseases with TCM [ 31 ]. In this study, we explored the potential mechanism of SZS in COVID-19 treatment by utilizing integrating network pharmacological approaches.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Potential Mechanism Governing the Effect of the Shen Zhu San on COVID‐19 by Network Pharmacology

Wang

Zhuo

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Since December 2019, coronavirus disease 2019 (COVID-19) due to SARS-CoV-2 infection has emerged in Wuhan and rapidly spread throughout China and even to other countries. Combined therapy with modern medicine and traditional Chinese medicine has been proposed, in which Shen Zhu San (SZS) was regarded as one of the basic prescriptions. Methods. Network pharmacological approaches along with candidate compound screening, target prediction, target tissue location, protein-protein interaction network, gene ontology (GO), KEGG enrichment analyses, and gene microarray analyses were applied. Results. A total of 627 targets of the 116 active ingredients of SZS were identified. Targets in immune cells and tissues were much more abundant than those in other tissues. A total of 597 targets were enriched in the GO biological cellular process, while 153 signaling pathways were enriched according to the KEGG analysis. A total of 450 SARS-related targets were integrated and intersected with the targets of SZS to identify 40 common targets that were significantly enriched in five immune function aspects of the immune system process during GO analysis. Several inflammation-related pathways were found to be significantly enriched throughout the study. Conclusions. The therapeutic mechanisms of the effects of SZS on COVID-19 potentially involve four effects: suppressing cytokine storms, protecting the pulmonary alveolar-capillary barrier, regulating the immune response, and mediating cell death and survival.

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The atherosclerosis-ameliorating effects and molecular mechanisms of BuYangHuanWu decoction

Cited by 41 publications

References 38 publications

The Enhanced Pharmacological Effects of Modified Traditional Chinese Medicine in Attenuation of Atherosclerosis Is Driven by Modulation of Gut Microbiota

The Enhanced Pharmacological Effects of Modified Traditional Chinese Medicine in Attenuation of Atherosclerosis Is Driven by Modulation of Gut Microbiota

Deciphering the Key Pharmacological Pathways and Targets of Yisui Qinghuang Powder That Acts on Myelodysplastic Syndromes Using a Network Pharmacology‐Based Strategy

Investigation of the Potential Mechanism Governing the Effect of the Shen Zhu San on COVID‐19 by Network Pharmacology

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