The Asymmetric Expression of SAUR Genes Mediated by ARF7/19 Promotes the Gravitropism and Phototropism of Plant Hypocotyls

Wang, Xiaoyi; Yu, Renbo; Wang, Jiajun; Lin, Zhou; Han, Xue; Deng, Zhaoguo; Fan, Liu‐Min; He, Hang; Deng, Xing Wang; Chen, Haodong

doi:10.1016/j.celrep.2020.107529

Cited by 38 publications

(50 citation statements)

References 49 publications

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“…Auxin is a typical phytohormone involved in plant developmental processes such as embryo morphogenesis, cell division and elongation, vascular tissue differentiation, lateral root initiation, geotropism and phototropism, among others 40 – 46 . Previous studies have revealed that IAA, ARG, GH3.1, and SAURs are key proteins affecting gravitropic and auxin-mediated growth responses in Arabidopsis 42 – 44 .…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have revealed that IAA, ARG, GH3.1, and SAURs are key proteins affecting gravitropic and auxin-mediated growth responses in Arabidopsis 42 – 44 . The asymmetric expression of SAUR genes in Arabidopsis facilitates gravitropism and phototropism of hypocotyls by promoting cell elongation 45 , 46 . Several SAUR family genes, including ARG7s ( indole-3-acetic acid-induced protein ) ( Pm021879 , Pm021884 , Pm021062 , Pm021877 , Pm021896 ) and SAUR20 ( SMALL AUXIN UP RNA 20 , Pm021015 ), were down-regulated in weeping stems (W ut vs. U ut ) (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Comparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in Prunus mume

Zhang

Zheng

et al. 2021

Sci Rep

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Prunus mume (also known as Mei) is an important ornamental plant that is popular with Asians. The weeping trait in P. mume has attracted the attention of researchers for its high ornamental value. However, the formation of the weeping trait of woody plants is a complex process and the molecular basis of weeping stem development is unclear. Here, the morphological and histochemical characteristics and transcriptome profiles of upright and weeping stems from P. mume were studied. Significant alterations in the histochemical characteristics of upright and weeping stems were observed, and the absence of phloem fibres and less xylem in weeping stems might be responsible for their inability to resist gravity and to grow downward. Transcriptome analysis showed that differentially expressed genes (DEGs) were enriched in phenylpropanoid biosynthesis and phytohormone signal transduction pathways. To investigate the differential responses to hormones, upright and weeping stems were treated with IAA (auxin) and GA3 (gibberellin A3), respectively, and the results revealed that weeping stems had a weaker IAA response ability and reduced upward bending angles than upright stems. On the contrary, weeping stems had increased upward bending angles than upright stems with GA3 treatment. Compared to upright stems, interestingly, DEGs associated with diterpenoid biosynthesis and phenylpropanoid biosynthesis were significantly enriched after being treated with IAA, and expression levels of genes associated with phenylpropanoid biosynthesis, ABC transporters, glycosylphosphatidylinositol (GPI)—anchor biosynthesis were altered after being treated with GA3 in weeping stems. Those results reveal that multiple molecular mechanisms regulate the formation of weeping trait in P. mume, which lays a theoretical foundation for the cultivation of new varieties.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in Prunus mume

Zhang

Zheng

et al. 2021

Sci Rep

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“…The auxin-mediated cell elongation response to unilateral light, shade, neighbour proximity signals and high temperature depends on enhanced expression of SAUR19-24, members of the SMALL AUXIN UP RNA family [13,62,73,101]. In the Arabidopsis hypocotyl SAUR19 expression is limited to the epidermis which is the cell layer that restricts hypocotyl growth [87], and in unilateral blue light SAUR19 expression only occurs on the shaded side of the hypocotyl [62]. Activation of SAUR19 stimulates H + -ATPase proton pumps, which leads to rapid apoplast acidification and acid growth (Figure 1) [48][49][50].…”

Section: Auxin-modulated Cell Growthmentioning

confidence: 99%

“…This NPH3 dephosphorylation asymmetry is mirrored by auxin reporters [ 10 , 43 , 44 , 46 ]. Asymmetric auxin concentrations allow for phototropic hypocotyl bending through auxin-mediated cell elongation on the shaded side of the hypocotyl [ 62 ]. Although most research regarding phototropism has focused on hypocotyl bending, phototropin-mediated bending towards blue light also occurs in inflorescence stems and petioles [ 63 ].…”

Section: Photoreceptors To Sense Temperature and Light Quality Qumentioning

confidence: 99%

Photoreceptors Regulate Plant Developmental Plasticity through Auxin

Küpers

Oskam

Pierik

2020

Plants

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Light absorption by plants changes the composition of light inside vegetation. Blue (B) and red (R) light are used for photosynthesis whereas far-red (FR) and green light are reflected. A combination of UV-B, blue and R:FR-responsive photoreceptors collectively measures the light and temperature environment and adjusts plant development accordingly. This developmental plasticity to photoreceptor signals is largely regulated through the phytohormone auxin. The phytochrome, cryptochrome and UV Resistance Locus 8 (UVR8) photoreceptors are inactivated in shade and/or elevated temperature, which releases their repression of Phytochrome Interacting Factor (PIF) transcription factors. Active PIFs stimulate auxin synthesis and reinforce auxin signalling responses through direct interaction with Auxin Response Factors (ARFs). It was recently discovered that shade-induced hypocotyl elongation and petiole hyponasty depend on long-distance auxin transport towards target cells from the cotyledon and leaf tip, respectively. Other responses, such as phototropic bending, are regulated by auxin transport and signalling across only a few cell layers. In addition, photoreceptors can directly interact with components in the auxin signalling pathway, such as Auxin/Indole Acetic Acids (AUX/IAAs) and ARFs. Here we will discuss the complex interactions between photoreceptor and auxin signalling, addressing both mechanisms and consequences of these highly interconnected pathways.

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“…Extensive studies have suggested that ARF proteins are involved in distinct developmental processes. In Arabidopsis , numerous ARF genes have been implicated in embryogenesis (ARF5 and ARF17) (Mallory et al ., 2005), root growth (ARF7, ARF10, ARF16, and ARF19) (Okushima et al ., 2005; Fukaki and Tasaka, 2009; Orosa-Puente et al ., 2018; Lee et al ., 2019), hypocotyl growth (ARF6, ARF7, ARF8, and ARF19) (Liu et al ., 2018; Reed et al ., 2018; Wang et al ., 2020), shoot regeneration (ARF4 and ARF5) (Zhang et al ., 2021), flower development (ARF2, ARF3, ARF6, and ARF8) (Nagpal et al ., 2005; Finet et al ., 2010), and senescence (ARF1 and ARF2) (Ellis et al ., 2005). In the case of rice, genetic studies show that the functions of ARFs are different from the functions of Arabidopsis .…”

Section: Introductionmentioning

confidence: 99%

Hydrogen sulfide improves the cold stress resistance through the CsARF5-CsDREB3 module in cucumber

Zhang

et al. 2021

Preprint

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Hydrogen sulfide (H2S) plays a crucial role in regulating cold tolerance. But the synergistic regulation of H2S and auxin in the plant response to cold stress has not been reported. In the study, we found that sodium hydrosulfide (NaHS, an H2S donor) treatment enhanced the cold tolerance of cucumber seedlings and increased the level of auxin. CsARF5, a cucumber auxin response factor (ARF) gene was isolated and its role in regulating H2S-mediated cold stress tolerance was described. Transgenic cucumber leaves overexpressing CsARF5 were obtained. Physiological analysis indicated that overexpression of CsARF5 enhanced the cold stress tolerance of cucumber and the regulation of the cold stress response by CsARF5 depends on H2S. In addition, molecular assays showed that CsARF5 modulated cold stress response by directly activating the expression of the dehydration-responsive element-binding (DREB)/C-repeat binding factor (CBF) gene CsDREB3, which was identified as a positive regulator of cold stress. Taken together, our results suggest that CsARF5 plays an important role in H2S-mediated cold stress in cucumber. These results shed light on the molecular mechanism by which H2S regulates cold stress response by mediating auxin signaling, and will provide insights for further studies on the molecular mechanism by which H2S regulates cold stress.

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The Asymmetric Expression of SAUR Genes Mediated by ARF7/19 Promotes the Gravitropism and Phototropism of Plant Hypocotyls

Cited by 38 publications

References 49 publications

Comparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in Prunus mume

Comparative gene expression analysis reveals that multiple mechanisms regulate the weeping trait in Prunus mume

Photoreceptors Regulate Plant Developmental Plasticity through Auxin

Hydrogen sulfide improves the cold stress resistance through the CsARF5-CsDREB3 module in cucumber

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