We aimed to investigate the relationship among urinary stone disease, androgenetic alopecia (AGA), and serum testosterone (T) levels. Between January 2008 and November 2015, we retrospectively investigated the biochemical parameters and anthropometric characteristics (height and weight) of 200 patients who had urinary tract stones. For the control group, we selected at random 168 participants who had no history of urolithiasis. Demographic data, such as participants' age, body mass index (BMI), hypertension (HT), diabetes mellitus (DM), baldness pattern, and serum T level were recorded. All participants were male, over 18 and under 60 years of age. We determined that risk of urolithiasis increased 1.3-fold in patients with vertex pattern alopecia and 2.1-fold in patients with total alopecia compared with those with no hair loss. In the severe balding Groups (Group III and Group IV), presence of testosterone deficiency was more frequent in patients with urinary stone disease (P = 0.041, OR = 2.38). Although in the non- balding and mild balding Groups (Group I and Group II), presence of testosterone deficiency was also more frequent in patients with urinary stone disease, a statistically significant difference was not seen. Significantly, we found that the presence of testosterone deficiency was more frequent in patients with urinary stone disease and who had severe AGA.