The Association of UNC13B Gene Polymorphisms and Diabetic Kidney Disease in a Chinese Han Population

Abstract

Background: Polymorphisms in the UNC13B gene are associated with diabetic kidney disease (DKD) in the European population. Asian populations are more likely to suffer from complications of type 2 diabetes mellitus (T2DM), including diabetic kidney disease (DKD). This case-control study aimed to investigate the association between UNC13B gene polymorphisms and DKD in a Chinese Han population. Material/Methods: Five single nucleotide polymorphism (SNP) loci (rs13293564, rs17360668, rs10114937, rs661712, and rs2281999) were genotyped in the UNC13B gene in 600 Chinese Han subjects. The study population included patients with T2DM with DKD (N=292) and control patients with T2DM without DKD (N=308). SNP genotyping was performed using a Sequenom MassARRAY system using chip-based matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF MS). Results: There were no significant differences in the distribution of allele or genotype frequencies in the five UNC13B SNP markers (rs13293564, rs17360668, rs10114937, rs661712, and rs2281999) between the DKD group and control group of patients with T2DM. Haplotype analysis identified eight haplotypes for the combined effect of the five SNP markers in the UNC13B gene. The haplotype GGCCG was significantly associated with an increased risk of DKD. Conclusions: This was the first study to demonstrate an association between UNC13B gene polymorphisms and the susceptibility to DKD in a Chinese Han population with T2DM. The haplotype GGCCG was significantly associated with an increased risk of DKD. The findings highlight the joint effect of SNP markers in the pathogenesis of DKD.

Development of a biomolecular approach to identify sperm functions and fertility using sperm RNAs

“…However, our present study suggests that SLC9A3R1 may have a broader role beyond capacitation. The UNC13B protein, which is primarily found in neurons and is associated with vesicle maturation through its diacylglycerol (DAG) domain ( Wang et al, 2019 ), is closely related to the acrosome reaction in mammalian spermatozoa, a crucial process for normal fertilization. In our current study, we found a correlation between UNC13B mRNA expression and sperm motility.…”

The correlation between serum uric acid and diabetic kidney disease in type 1 diabetes patients in Anhui, China

“…Diabetic kidney disease (DKD) is one of the most common chronic kidney diseases (CKD), closely related to end-stage renal disease (ESRD) and increased risk of cardiovascular disease (CVD) in type 1 diabetes mellitus (T1DM) [ 1 – 3 ]. The incidence and distribution of T1DM patients in China differed from that in Europe and America [ 4 ], and the previous evidence showed that DKD characteristics could be heterogeneous among patients from different ethnicities [ 5 – 7 ]. It is essential to explore the association between modifiable factors and DKD for delaying the progression of DKD in other races.…”