The association of type II diabetes with gut microbiota composition

Navab-Moghadam, Fatemeh; Sedighi, Mansour; Khamseh, Mohammad E.; Alaei-Shahmiri, Fariba; Talebi, Malihe; Razavi, Shabnam; Amirmozafari, Nour

doi:10.1016/j.micpath.2017.07.034

Cited by 93 publications

(42 citation statements)

References 46 publications

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“…In humans, decreased levels of Prevotella copri and Faecalibacterium prausnitzii populations were observed in severe forms of chronic kidney disease (CKD), which also negatively correlate with the presence of important diagnostic markers, such as C-reactive protein and cystatin C levels [49][50][51]. Similarly, Prevotella copri and Faecalibacterium prausnitzii were significantly depleted in humans with diabetic nephropathy [43,52]. Conversely, the symptoms of diabetes and inflammation were also shown to be ameliorated upon the administration of Faecalibacterium prausnitzii to such patients [53], suggesting an important diagnostic and therapeutic relevance of these microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Microbiome-Metabolome Signature of Acute Kidney Injury

et al. 2020

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Intestinal microbiota play a considerable role in the host’s organism, broadly affecting its organs and tissues. The kidney can also be the target of the microbiome and its metabolites (especially short-chain fatty acids), which can influence renal tissue, both by direct action and through modulation of the immune response. This impact is crucial, especially during kidney injury, because the modulation of inflammation or reparative processes could affect the severity of the resulting damage or recovery of kidney function. In this study, we compared the composition of rat gut microbiota with its outcome, in experimental acute ischemic kidney injury and named the bacterial taxa that play putatively negative or positive roles in the progression of ischemic kidney injury. We investigated the link between serum creatinine, urea, and a number of metabolites (acylcarnitines and amino acids), and the relative abundance of various bacterial taxa in rat feces. Our analysis revealed an increase in levels of 32 acylcarnitines in serum, after renal ischemia/reperfusion and correlation with creatinine and urea, while levels of three amino acids (tyrosine, tryptophan, and proline) had decreased. We detected associations between bacterial abundance and metabolite levels, using a compositionality-aware approach—Rothia and Staphylococcus levels were positively associated with creatinine and urea levels, respectively. Our findings indicate that the gut microbial community contains specific members whose presence might ameliorate or, on the contrary, aggravate ischemic kidney injury. These bacterial taxa could present perspective targets for therapeutical interventions in kidney pathologies, including acute kidney injury.

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Section: Discussionmentioning

confidence: 99%

Microbiome-Metabolome Signature of Acute Kidney Injury

et al. 2020

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“…Although the characteristics of the DM gut microbiome are still mired in controversy, gut microbiome studies have hinted at the biological functions or pathogenic roles of gut microbiota in DM progression . The differential richness of specific bacteria is potentially correlated with DM development . Faecalibacterium prausnitzii ( F. prausnitzii ) are the predominant butyrate‐producing bacteria in the gastrointestinal tract.…”

Section: Introductionmentioning

confidence: 99%

“…[14][15][16] The differential richness of specific bacteria is potentially correlated with DM development. 17 Faecalibacterium prausnitzii (F. prausnitzii) are the predominant butyrateproducing bacteria in the gastrointestinal tract. Studies have revealed that F. prausnitzii are critical in host health and in many diseases.…”

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confidence: 99%

Faecalibacterium prausnitzii‐derived microbial anti‐inflammatory molecule regulates intestinal integrity in diabetes mellitus mice via modulating tight junction protein expression

Liang

Zhang

et al. 2019

Journal of Diabetes

108

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Background Impaired intestinal barrier structure and function have been validated as an important pathogenic process in type 2 diabetes mellitus (T2DM). Gut dysbiosis is thought to be the critical factor in diabetic intestinal pathogenesis. As the most abundant commensal bacteria, Faecalibacterium prausnitzii (F. prausnitzii) play important roles in gut homeostasis. The microbial anti‐inflammatory molecule (MAM), an F. prausnitzii metabolite, has anti‐inflammatory potential in inflammatory bowel disease (IBD). Thus, we aimed to explore the function and mechanism of MAM on the diabetic intestinal epithelium. Methods 16S high‐throughput sequencing was used to analyze the gut microbiota of db/db mice (T2DM mouse model). We transfected a FLAG‐tagged MAM plasmid into human colonic cells to explore the protein‐protein interactions and observe cell monolayer permeability. For in vivo experiments, db/db mice were supplemented with recombinant His‐tagged MAM protein from E. coli BL21 (DE3). Results The abundance of F. prausnitzii was downregulated in the gut microbiota of db/db mice. Immunoprecipitation (IP) and mass spectroscopy (MS) analyses revealed that MAM potentially interacts with proteins in the tight junction pathway, including zona occludens 1 (ZO‐1). FLAG‐tagged MAM plasmid transfection stabilized the cell permeability and increased ZO‐1 expression in NCM460, Caco2, and HT‐29 cells. The db/db mice supplemented with recombinant His‐tagged MAM protein showed restored intestinal barrier function and elevated ZO‐1 expression. Conclusions Our study shows that MAM from F. prausnitzii can restore the intestinal barrier structure and function in DM conditions via the regulation of the tight junction pathway and ZO‐1 expression.

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“…and Faecalibacterium prausnitzii have been associated 5 with gut disease states, e.g. Crohn's disease 12 , type 1, type 2 and gestational diabetes [13][14][15] , celiac disease 16 and obesity 17 .…”

Section: Introductionmentioning

confidence: 99%

Exploring the Molecular Pathways Behind the Effects of Nutrients and Dietary Polyphenols on Gut Microbiota and Intestinal Permeability: A Perspective on the Potential of Metabolomics and Future Clinical Applications

Peron

Hidalgo‐Liberona

González‐Domínguez

et al. 2019

J. Agric. Food Chem.

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The gastrointestinal tract hosts the largest microbial population of the human body, which works in symbiosis with the host to provide several important functions and contributes to the maintenance of host health. The diet is one of the factors that can most affect the gut microbiota, with subsequent consequences on host health. One consequence of changes in microbiota is changes in intestinal permeability (IP); disruption of this latter is related to the development of several diseases and is a frequent condition in older people. Nevertheless, the molecular pathways regulating these effects are still unclear, and a comprehensive understanding of the dietary components that can affect IP is lacking. Metabolomics, that has been widely used to study the transformation of nutrients by intestinal microbiota, could be a suitable approach for this purpose. However, up to now, the research has focused mainly on dietary fibers and tryptophan, while the activity of dietary polyphenols remains almost completely unexplored. Hence, the aim here was to review the most recent literature concerning the application of metabolomics in the study of the correlation between diet-induced alterations of gut microbiota and the effects on intestinal permeability, with a particular focus on the discovery of the molecular pathways involved. An additional aim was to give a perspective on the future research involving dietary polyphenols, given that despite their potential implication for the prevention and treatment of several diseases related to increased intestinal permeability, few studies have been reported to date.

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The association of type II diabetes with gut microbiota composition

Cited by 93 publications

References 46 publications

Microbiome-Metabolome Signature of Acute Kidney Injury

Microbiome-Metabolome Signature of Acute Kidney Injury

Faecalibacterium prausnitzii‐derived microbial anti‐inflammatory molecule regulates intestinal integrity in diabetes mellitus mice via modulating tight junction protein expression

Exploring the Molecular Pathways Behind the Effects of Nutrients and Dietary Polyphenols on Gut Microbiota and Intestinal Permeability: A Perspective on the Potential of Metabolomics and Future Clinical Applications

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