The association of telomere length and telomerase activity with adverse outcomes in older patients with non-ST-elevation acute coronary syndrome

Abstract

Background Non-ST elevation acute coronary syndrome (NSTEACS) occurs more frequently in older patients with an increased occurrence of recurrent cardiac events following the index presentation. Telomeres are structures consisting of repeated DNA sequences as associated shelterin proteins at the ends of chromosomes. We aim to determine whether telomere length (TL) and telomerase activity (TA) predicted poor outcomes in older patients presenting with NSTEACS undergoing invasive care. Method Older patients undergoing invasive management for NSTEACS were recruited to the ICON-1 biomarker study (NCT01933581). Peripheral blood mononuclear cells (PBMC) were recovered on 153 patients. DNA was isolated and mean TL was measured by quantitative PCR expressed as relative T (telomere repeat copy number) to S (single copy gene number) ratio (T/S ratio), and a telomere repeat amplification assay was used to assess TA during index presentation with NSTEACS. Primary clinical outcomes consisted of death, myocardial infarction (MI), unplanned revascularisation, stroke and significant bleeding recorded at 1 year. TL and TA were divided into tertile groups for analysis. Cox proportional hazards regression was performed. Ordinal regression was performed to evaluate the relationship between TL and TA and traditional cardiovascular risk factors at baseline. Results 298 patients were recruited in the ICON-1 study of which 153 had PBMC recovered. The mean age was 81.0 ± 4.0 years (64% male). Mean telomere length T/S ratio was 0.47 ±

Disease-modifying effects of sodium selenate in a model of drug-resistant, temporal lobe epilepsy

“…Telomere length was investigated as a surrogate marker of epilepsy severity and response to pharmacological treatment ( Chan et al, 2020 ; Galletly et al, 2017 ; Kang et al, 2020 ; Lukens et al, 2009 ). Ear biopsies were taken 25 weeks post-SE to evaluate the effects of chronic epilepsy on telomere length.…”

Leukocyte Telomere Length as a Molecular Biomarker of Coronary Heart Disease

“…Chan et al [ 71 ] investigated LTL in 135 patients with ACS without ST elevations who underwent percutaneous coronary intervention. The mean age of the patients was 81 ± 4 years and 64% of them were men.…”

“…Studies of relative LTL in patients with AMI (predominantly) and with stable CAD (to a lesser extent) are in their infancy. Such studies are still in the minority, the sample sizes are small, and the results obtained in some studies are contradictory [ 60 , 69 , 70 , 71 ]. The ethnic and racial heterogeneity of the samples in the publications we analyzed draws attention, which does not allow us to assess the additional influences of ethnicity and region of residence of adults with and without the studied CVDs on the absolute and relative LTL.…”

The Correlation of Different Intensities Exercise on Telomere Length

“…Telomeres are reiterative DNA sequences (5'-TTAGGGn-3') that function to protect genomic DNA from enzymatic degradation 2,5,[16][17][18] . These repeated TTAGGG sequences are located at the end of chromosomes and tied up by a protective protein complex called Shelterin [19][20][21] . Furthermore, shelterin and the proteins that are involved in chromatin remodeling form telomere structure 4,6,20 .…”