The Association of Targeted Gut Microbiota with Body Composition in Type 2 Diabetes Mellitus

Hung, Wei‐Chun; Hung, Wei‐Wen; Tsai, Hung-Chin; Chang, Chen-Chia; Chiu, Yi‐Wen; Hwang, Shiuh-Lin; Kuo, Mei‐Chuan; Chen, Szu-Chia; Dai, Chia‐Yen; Tsai, Yi‐Chun

doi:10.7150/ijms.51164

Cited by 33 publications

(35 citation statements)

References 51 publications

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“…Among the common bacterial communities, Bacteroidetes, Firmicutes, Proteobacteria, Actinobacteria, and Verrucomicrobia were the main five phyla present in the gut microbiota of all groups (Figures 6A,B). Many studies have shown that obesity and diabetes mellitus are closely related to the ratio of Firmicutes to Bacteroidetes (Ley et al, 2005;Grigor'eva, 2020;Hung et al, 2021). However, the results revealed a slight increase of the Firmicutes/Bacteroidetes ratio in db/db mice than in WT mice at each age, but without significant difference (Figure 6C).…”

Section: Differentially Represented Bacterial Taxa In Db/db Micementioning

confidence: 86%

Gut Microbiota Alteration Is Associated With Cognitive Deficits in Genetically Diabetic (Db/db) Mice During Aging

Zhang

Yuan

et al. 2022

Front. Aging Neurosci.

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Recent studies have revealed that the microbiota may be implicated in diabetes-related cognitive dysfunction. However, the relationship between gut microbiota and cognitive dysfunction during the progression of type 2 diabetes remains elusive. We used 16S rRNA sequencing combined with conventional behavioral tests to explore the longitudinal changes of gut microbiota and cognition in diabetic db/db mice (leptin receptor knockout mice) and their wild-type littermates at different ages. Prussian blue staining was performed to detect the microhemorrhage in the brain, and immunofluorescent study was applied to analyze microglia activation. Moreover, a Meso Scale Discovery kit was used to determine the cytokine levels in the brain. Db/db mice exhibited age dependent pathological characteristics, including cognitive deficits, neuron damage, spontaneous hemorrhages and neuroinflammation. Furthermore, we observed that the diversity and composition of gut microbiota significantly differed between the wild-type and db/db mice during aging. We found that compared to age-matched wild-type mice, genus Helicobacter was significant higher in db/db mice at 18 and 26 weeks. Correlation analysis revealed that Helicobacter is positively associated with Iba-1 positive cells and TNF-α expression. Collectively, our longitudinal study suggests that diabetic cognitive impairment during aging is associated with abnormal gut microbiota composition, which may play a role in the regulation of neuroinflammation.

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Section: Differentially Represented Bacterial Taxa In Db/db Micementioning

confidence: 86%

Gut Microbiota Alteration Is Associated With Cognitive Deficits in Genetically Diabetic (Db/db) Mice During Aging

Zhang

Yuan

et al. 2022

Front. Aging Neurosci.

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“…The detailed methods were described previously [ 33 ]. In brief, fecal samples were collected at the same day of blood and urine collection.…”

Section: Methodsmentioning

confidence: 99%

“…We used real-time qPCR in a StepOnePlus Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA) to detect bacterial 16S rRNA gene copies in stool according to a previous study [ 26 , 33 ]. The 8 pairs of 16S rRNA gene primers specific to Firmicutes , Bacteroides , the C. leptum group , F. prausnitzii , Bifidobacterium , E. coli , and A. muciniphila are listed in Table S1 .…”

Section: Methodsmentioning

confidence: 99%

Gut Microbiota and Non-Alcoholic Fatty Liver Disease Severity in Type 2 Diabetes Patients

Tsai

Hung

et al. 2021

JPM

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Introduction: Non-alcoholic fatty liver disease (NAFLD) remains an important health issue worldwide. The increasing prevalence of NAFLD is linked to type 2 diabetes (T2D). The gut microbiota is associated with the development of NAFLD and T2D. However, the relationship between gut microbiota and NAFLD severity has remained unclear in T2D patients. The aim of this study was to evaluate the relationship of gut microbiota with the severity of NAFLD in T2D patients. Methods: This cross-sectional study used transient elastography (FibroScan) to evaluate the severity of hepatic steatosis. We utilized qPCR to measure the abundance of Bacteroidetes, Firmicutes, Faecalibacterium prausnitzii, Clostridium leptum group, Bacteroides, Bifidobacterium, Akkermansia muciniphila, and Escherichia coli. Results: Of 163 T2D patients, 83 with moderate to severe NAFLD had higher abundance of bacteria of the phylum Firmicutes with respect to 80 patients without NAFLD or with mild NAFLD. High abundance of the phylum Firmicutes increased the severity of NAFLD in T2D patients. A positive correlation between NAFLD severity and the phylum Firmicutes was found in T2D male patients with body mass index ≥24 kg/m2 and glycated hemoglobin <7.5%. Conclusion: Enrichment of the fecal microbiota with the phylum Firmicutes is significantly and positively associated with NAFLD severity in T2D patients. The gut microbiota is a potential predictor of NAFLD severity in T2D patients.

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“…In addition to the aforementioned positive effects of regular physical activity on glycemic control/insulin sensitivity, exercise training (together with other lifestyle changes) is known to contribute to changes in body composition (increases in muscle mass and reductions in fat mass) [37,38]. It is important to note that a changed body composition can result in a different microbiome composition [39].…”

Section: Influence Of Exercise Training On the Gut Microbiota And The...mentioning

confidence: 99%

Exercise for the Diabetic Gut—Potential Health Effects and Underlying Mechanisms

Valder

Brinkmann

2022

Nutrients

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It can be assumed that changes in the gut microbiota play a crucial role in the development of type 2 diabetes mellitus (T2DM). It is generally accepted that regular physical activity is beneficial for the prevention and therapy of T2DM. Therefore, this review analyzes the effects of exercise training on the gut microbiota composition and the intestinal barrier function in T2DM. The current literature shows that regular exercise can influence the gut microbiota composition and the intestinal barrier function with ameliorative effects on T2DM. In particular, increases in the number of short-chain fatty acid (SCFA)-producing bacteria and improvements in the gut barrier integrity with reduced endotoxemia seem to be key points for positive interactions between gut health and T2DM, resulting in improvements in low-grade systemic inflammation status and glycemic control. However, not all aspects are known in detail and further studies are needed to further examine the efficacy of different training programs, the role of myokines, SCFA-producing bacteria, and SCFAs in the relevant metabolic pathways. As microbial signatures differ in individuals who respond differently to exercise training programs, one scientific focus could be the development of computer-based methods for the personalized analysis of the gut microbiota in the context of a microbiota/microbiome-based training program.

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The Association of Targeted Gut Microbiota with Body Composition in Type 2 Diabetes Mellitus

Cited by 33 publications

References 51 publications

Gut Microbiota Alteration Is Associated With Cognitive Deficits in Genetically Diabetic (Db/db) Mice During Aging

Gut Microbiota Alteration Is Associated With Cognitive Deficits in Genetically Diabetic (Db/db) Mice During Aging

Gut Microbiota and Non-Alcoholic Fatty Liver Disease Severity in Type 2 Diabetes Patients

Exercise for the Diabetic Gut—Potential Health Effects and Underlying Mechanisms

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