The Association of pre-miR27a Gene Polymorphism and Clinicopathological Data in Thai Breast Cancer Patients

Sanguansin, Sirima; Saelee, Pensri; Kritsiriwuthinan, Kanyanan; Pongstaporn, Wanida

doi:10.31557/apjcp.2023.24.6.2055

Cited by 2 publications

(2 citation statements)

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“…This molecule can reportedly regulate the expression of multiple genes and plays a crucial role in the development of diseases [ 25 ]. Numerous studies have shown that miR27a plays a critical role in various diseases, including hyperlipidemia [ 26 ], breast cancer [ 27 ], and rectal cancer [ 28 ]. miR27a may utilized as a potential biomarker for these diseases.…”

Section: Discussionmentioning

confidence: 99%

YTHDF2-Mediated m6A methylation inhibition by miR27a as a protective mechanism against hormonal osteonecrosis in BMSCs

Yuan,

Liu,

Abudoukadier

et al. 2024

BMC Musculoskelet Disord

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Background With the increasing incidence of steroid-induced necrosis of the femoral head (SNFH), numerous scholars have investigated its pathogenesis. Current evidence suggests that the imbalance between lipogenesis and osteoblast differentiation in bone marrow mesenchymal stem cells (BMSCs) is a key pathological feature of SNFH. MicroRNAs (miRNAs) have strong gene regulatory effects and can influence the direction of cell differentiation. N6-methyladenosine (m6A) is a prevalent epigenetic modification involved in diverse pathophysiological processes. However, knowledge of how miRNAs regulate m6A-related factors that affect BMSC differentiation is limited. Objective We aimed to investigate the role of miR27a in regulating the expression of YTHDF2 in BMSCs. Methods We compared miR27a, YTHDF2, and total m6A mRNA levels in SNFH-affected and control BMSCs. CCK-8 and TUNEL assays were used to assess BMSC proliferation and apoptosis. Western blotting and qRT‒PCR were used to measure the expression of osteogenic (ALP, RUNX2, and OCN) and lipogenic (PPARγ and C/EBPα) markers. Alizarin Red and Oil Red O staining were used to quantify osteogenic and lipogenic differentiation, respectively. miR27a was knocked down or overexpressed to evaluate its impact on BMSC differentiation and its relationship with YTHDF2. Bioinformatics analyses identified YTHDF2 as a differentially expressed gene in SNFH (ROC analysis) and revealed potential signaling pathways through GSEA. The effects of YTHDF2 silencing on the lipogenic and osteogenic functions of BMSCs were assessed. Results miR27a downregulation and YTHDF2 upregulation were observed in the SNFH BMSCs. miR27a knockdown/overexpression modulated YTHDF2 expression, impacting BMSC differentiation. miR27a silencing decreased m6A methylation and promoted osteogenic differentiation, while YTHDF2 silencing exerted similar effects. GSEA suggested potential signaling pathways associated with YTHDF2 in SNFH. Conclusion miR27a regulates BMSC differentiation through YTHDF2, affecting m6A methylation and promoting osteogenesis. This finding suggests a potential therapeutic target for SNFH.

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Section: Discussionmentioning

confidence: 99%

YTHDF2-Mediated m6A methylation inhibition by miR27a as a protective mechanism against hormonal osteonecrosis in BMSCs

Yuan,

Liu,

Abudoukadier

et al. 2024

BMC Musculoskelet Disord

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“…Additionally, investigations have linked miR-34 rs4938723 and miR-195-5p to the prognosis of colorectal cancer (14,15). Moreover, research findings indicate that single nucleotide polymorphisms in miRNAs are correlated with the prognosis of various types of tumors (16)(17)(18)(19). While numerous studies have established associations between miRNA single nucleotide polymorphisms and the prognosis of various types of tumors, limited attention has been directed toward exploring the connection between miRNA single nucleotide polymorphisms and the prognosis of gastric cancer.…”

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confidence: 99%

MiRNA polymorphisms affect the prognosis of gastric cancer: insights from Xianyou, Fujian

Wu,

Zhang,

Lyu

et al. 2024

Front. Oncol.

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IntroductionGastric cancer, characterized by high incidence and substantial disease burden, has drawn continuous attention regarding its occurrence and prognosis. Genetics plays a crucial role in influencing the prognosis of gastric cancer, and single nucleotide polymorphisms are closely associated with the occurrence, development, and prognosis of this malignant tumor. Our study aims to conduct survival analysis on patients carrying different single nucleotide polymorphisms, exploring the relationship between miRNA single nucleotide polymorphisms and the prognosis of gastric cancer.MethodsGenetic data from 344 patients in Xianyou, Fujian, formed the basis of our study. We delineated the survival rate and median survival time, utilizing the log-rank test and COX regression analysis as statistical tools.ResultsUpon stratifying the data by sex or operation, it was discerned that the GG genotype at MSH2 rs17502941 independently posed a heightened risk for gastric cancer. Other stratification analyses suggested that the subsequent single nucleotide polymorphisms were correlated with patient prognosis: rs17502941, rs884225, rs1468063, rs7143252, and rs2271738.DiscussionThe outcomes of this study strongly suggest that miRNA polymorphisms significantly influence the survival time of gastric cancer patients and can serve as effective predictors for the prognosis of gastric cancer.

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The Association of pre-miR27a Gene Polymorphism and Clinicopathological Data in Thai Breast Cancer Patients

Cited by 2 publications

References 14 publications

YTHDF2-Mediated m6A methylation inhibition by miR27a as a protective mechanism against hormonal osteonecrosis in BMSCs

YTHDF2-Mediated m6A methylation inhibition by miR27a as a protective mechanism against hormonal osteonecrosis in BMSCs

MiRNA polymorphisms affect the prognosis of gastric cancer: insights from Xianyou, Fujian

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