The Association of MMP-9 Enzyme Activity, MMP-9 C1562T Polymorphism, and MMP-2 and -9 and TIMP-1, -2, -3, and -4 Gene Expression in Lung Cancer

Bayramoglu, Aysegul; Güneş, Hasan Veysi; Metintaş, Muzaffer; Degirmenci, I.; Mutlu, Fezan; Alataş, Füsun

doi:10.1089/gtmb.2009.0053

Cited by 46 publications

(36 citation statements)

References 33 publications

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“…Genotypes for the C1562T polymorphism of MMP-9 were determined by polymerase chain reaction as described previously (Bayramoglu et al 2009) using the primers 5 0 -GCCTGGCACATAGTAGGC CC-3 0 (sense) and 5 0 -TTCCTAGCCAGCCGGCATC-3 0 (antisense). A 435-bp PCR product was cleaved with 1U SphI restriction enzyme (New England Biolabs, Ontario, Canada).…”

Section: Genotype Determinationmentioning

confidence: 99%

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Is there any genetic predisposition of MMP-9 gene C1562T and MTHFR gene C677T polymorphisms with essential hypertension?

et al. 2013

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The current study was conducted to determine whether there is a relation between hypertension and two different polymorphisms, including C1562T of the Matrix metalloproteinase-9 (MMP-9) gene and C677T of the methylenetetrahydrofolate reductase (MTHFR) gene. Genomic DNA obtained from 224 persons (125 patients with hypertension and 99 healthy controls) were used in the study. Polymorphisms were determined by using polymerase chain reaction-restriction fragment length polymorphism and electrophoresis. The results were statistically analyzed and were found to be statistically significant. The frequencies of the C1562T genotypes were found to be, in controls CC 75.8 % and CT 24.2 % and in patients CC 71.2 %, and CT 28.8 %. The frequencies of C677T genotype were found to be, in controls CC 56.6 %, CT 38.4 and TT 5.1 % in controls and in patients CC 52 %, CT 30.4 % and TT 17.6 %. In conclusion, we may suggest that there is no relation between the essential hypertension and C1562T polymorphism of MMP-9 gene; on the other hand C677T polymorphism (genotype TT) of MTHFR gene can be regarded as a genetic indicator for the development of essential hypertension.

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Section: Genotype Determinationmentioning

confidence: 99%

“…It degrades type IV collagen, proteoglycan, and laminin. MMP-9 is produced by tumoral cells as well as inflammatory cells such as neutrophil, eosinophil, monocyte, lymphocyte and alveolar macrophage (Chintala et al 1999;Corbel et al 2000;Apakkan Aksun et al 2001;Bayramoglu et al 2009Bayramoglu et al , 2011.…”

Section: Introductionmentioning

confidence: 99%

Is there any genetic predisposition of MMP-9 gene C1562T and MTHFR gene C677T polymorphisms with essential hypertension?

et al. 2013

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“…Matrix metalloproteinases (MMPs) have long been linked with cancer cell invasion and metastasis (15). Matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) are main members of MMPs and patients with high MMP-2 or MMP-9 expression have poorer overall survival (16)(17)(18). Nicotine increased the activity of MMP-2 in esophageal squamous…”

Section: Introductionmentioning

confidence: 99%

Flavonoid components in Scutellaria baicalensis inhibit nicotine-induced proliferation, metastasis and lung cancer-associated inflammation in vitro

Gong

Liu

et al. 2014

International Journal of Oncology

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Abstract. The objective of the present study was to investigate the therapeutic efficacy of flavonoid components in Scutellaria baicalensis on proliferation, metastasis and lung cancer-associated inflammation during nicotine induction in the A549 and H1299 lung cancer cell lines. After experimental period, augmentation of proliferation was observed, accompanied by marked decrease in apoptotic cells in nicotine-induced lung cancer cells; additionally, nicotine-exposed cells exhibited increased invasive and migratory abilities based on invasion and wound-healing assay. Flavones in Scutellaria, baicalin, baicalein and wogonin significantly counteracted the above deleterious changes. Moreover, assessment of tumor apoptotic and metastatic factors on mRNA levels by quantitative PCR and protein levels by western blotting revealed that these phytochemical treatments effectively negated nicotine-induced upregulated expression of bcl-2, bcl-2/bax ratio, caspase-3, matrix metalloproteinase (MMP)-2 and MMP-9 as well as downregulated expression of bax. Further analysis of inflammatory markers such as tumor necrosis factor (TNF)-α and interleukin (IL)-6 in cell culture supernatant and mRNA and protein expression of nuclear transcription factor-kappaB (NF-κB) and I kappa B-alpha (IκB-α) was carried out to substantiate the anti-inflammatory effect of flavones in Scutellaria in nicotine-exposed lung cancer cells. The therapeutic effects observed in the present study are attributed to the potent potential against proliferation, metastasis and inflammatory microenvironment by flavonoid components in Scutellaria in nicotine-induced lung cancer cells.

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“…regions of other MMPs such as MMP-1 and MMP-9, may serve as promising markers for the prediction of lung cancer susceptibility and prognosis (30)(31)(32). From the molecular viewpoint, MMP-8 might increase cell adhesion by rearrangement of cytoskeleton actin, thus decreasing cell invasion (33).…”

Section: Discussionmentioning

confidence: 99%

The Contribution of MMP-8 Promoter Genotypes to Childhood Leukemia

Pei

Chang

Hsu

et al. 2017

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The Association of MMP-9 Enzyme Activity, MMP-9 C1562T Polymorphism, and MMP-2 and -9 and TIMP-1, -2, -3, and -4 Gene Expression in Lung Cancer

Cited by 46 publications

References 33 publications

Is there any genetic predisposition of MMP-9 gene C1562T and MTHFR gene C677T polymorphisms with essential hypertension?

Is there any genetic predisposition of MMP-9 gene C1562T and MTHFR gene C677T polymorphisms with essential hypertension?

Flavonoid components in Scutellaria baicalensis inhibit nicotine-induced proliferation, metastasis and lung cancer-associated inflammation in vitro

The Contribution of MMP-8 Promoter Genotypes to Childhood Leukemia

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