“…Sediment samples containing natural microbial communities were obtained from the middle of a wetland in Anglesey, UK, through which a small river (southern Afon Goch) flows, and which receives AMD pollution from an abandoned Cu mine at Parys Mountain (Dean et al, 2013;Aguinaga et al, 2018). These surface sediments are rich in trace metals (0.2 -10 mg g -1 Al, 0.2 -0.6 mg g -1 As, 1 -5 mg g -1 Cu, 90 -140 mg g -1 Fe, 0.1 -2 mg g -1 Pb, 0.5 -3 mg g -1 Zn), and sulfur (3 -8 mg g -1 S), and with dissolved organic carbon (DOC) and nitrogen concentrations of approximately 0.5 mg g -1 and 0.01 mg g -1 , respectively (Aguinaga et al, 2019). They are composed of light brown, medium-to-fine sediment and contain larger soil particles and plant debris.…”