The association of glutathione-S-transferase gene polymorphisms (GSTM1, GSTT1, GSTP1) with idiopathic male infertility

Safarinejad, Mohammad Reza; Shafiei, Nayyer; Safarinejad, Shiva

doi:10.1038/jhg.2010.59

Cited by 69 publications

(66 citation statements)

References 38 publications

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“…Likewise, two more studies in the latter relation have been performed in Iran. The first, performed by Safarinejad et al, had surprising and unexpected results [22], but the second one, performed by Salehi et al, showed a correlation between idiopathic male infertility and GSTT1 and GSTM1 null deletions in men from Northern Iran [23]. Contrary to the study by Safarinejad et al we found no individual with hetero-or homozygote genotypes in codon 105 of GSTP1 (GSTP1 313 A/G and 313 G/G polymorphisms).…”

Section: Discussioncontrasting

confidence: 55%

The Association of Seminal Plasma Antioxidant Levels and Sperm Chromatin Status with Genetic Variants ofGSTM1andGSTP1(Ile105Val and Ala114Val) in Infertile Men with Oligoasthenoteratozoospermia

et al. 2013

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Abstract. In this study we aimed to examine the effects of genetic variants of GSTM1 and GSTP1 (Ile105Val and Ala114Val) on GST activity, seminal oxidative stress and sperm chromatin status in infertile men with oligoasthenoteratozoospermia (OAT). The study population (n = 121) consisted of 95 infertile men with OAT and 26 controls with normozoospermia. Multiplex polymerase chain reaction (PCR) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were utilized to detect the aforesaid genetic variants. We measured GST activity and total antioxidant capacity (TAC) of seminal plasma by spectrophotometry. Sperm chromatin integrity and maturity were assessed using toluidine blue and chromomycin A3 (CMA3-positive sperm) staining, respectively. The analysis showed that subgroups of GSTM1 null and GSTP1 C/T+T/T genotypes in comparison with GSTM1 present and GSTP1 wild type (C/C) genotypes did not have statistically significant differences in both OAT or normozoospermic men considering sperm concentration and motility, percentage of CMA3-positive sperm, seminal plasma TAC, sperm chromatin integrity and GST activity. Thus, the findings of our study suggest that there are no significant associations between GSTM1 and GSTP1 polymorphisms and sperm parameters at conventional or at molecular levels including OS status, sperm chromatin integrity or maturity in Iranian infertile men with OAT and normozoospermia. However, these polymorphisms could be related to the fertility status of the studied population but not evaluated in this study.

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Section: Discussioncontrasting

confidence: 55%

The Association of Seminal Plasma Antioxidant Levels and Sperm Chromatin Status with Genetic Variants ofGSTM1andGSTP1(Ile105Val and Ala114Val) in Infertile Men with Oligoasthenoteratozoospermia

et al. 2013

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“…Two other studies found that, the GSTP1 Ile/Ile genotype has been positively associated with PCa. 22,36 Mao et al examined the possible effect modification by the GSTP1 polymorphism on smoking and PCa. They found that in individuals with the Ile/Ile genotype, smoking was strongly associated with an increased risk of PCa.…”

Section: Discussionmentioning

confidence: 99%

Glutathione S-transferase gene polymorphisms (GSTM1, GSTT1, GSTP1) and prostate cancer: a case-control study in Tehran, Iran

Safarinejad¹,

Shafiei²,

Safarinejad³

2011

Prostate Cancer Prostatic Dis

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We evaluated the relationship between polymorphisms in the glutathione S-transferases (GSTs) GSTM1, GSTT1 and GSTP1 genes and prostate cancer (PCa). PCR-restriction fragment length polymorphism assay was used to genotype the GSTM1, GSTT1, and GSTP1 polymorphisms in 168 PCa cases and 336 frequency matched controls. The GSTM1 null, and GSTT1 null genotypes were associated with an increased odds ratio (OR) for PCa (OR ¼ 3.28, 95% confidence interval (CI): 2.47-5.64; P ¼ 0.005, and OR ¼ 3.21, 95% CI: 2.52-5.64; P ¼ 0.005, respectively) (Pcorrected ¼ 0.0062). The frequency of GSTP1 Val/Val genotype was 14.3% in cases compared with 2.4% in controls, this polymorphism thus being associated with a significantly increased risk of PCa (OR ¼ 3.72, 95% CI: 1.67-5.65; P ¼ 0.002). The risk associated with the concurrent absence of both of the genes (OR ¼ 4.8, 95% CI: 2.34-6.78) was greater than the product of risk in men with either null (OR ¼ 1.52, 95% CI: 0.82-2.31) genotype combinations (P ¼ 0.001, Pcorrected ¼ 0.0045). The combination of GSTP1 Ile/Val or Val/Val polymorphism with the GSTT1 null and GSTM1 null type resulted in an OR of 6.21 (95% CI: 4.83-16.87) (P ¼ 0.0001, Pcorrected ¼ 0.0062). A higher frequency of the GSTM1 null genotype and GSTT1 null genotype was observed in patients with Gleason score 47, with an OR for GSTM1 null 4.67 (95% CI: 3.64-7.62; P ¼ 0.001) and with an OR for GSTT1 null 3.62 (95% CI: 2.31-5.74; P ¼ 0.004). The results obtained demonstrated that simultaneous presence of three potentially risk alleles (GSTM1 null, GSTT1 null and GSTP1 Val) lead to a significant OR increase for PCa.

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“…The characteristics of selected studies are summarized in Table 1. A total of 12 eligible studies met the inclusion criteria, including 11 studies for GSTM1 [14,15,[25][26][27][28][29][30][31][32][33] and 9 ones for GSTT1 [17,26,27,[29][30][31][32][33]. All the included studies were case-control studies.…”

Section: Characteristics Of Eligible Studiesmentioning

confidence: 99%

Association of the Glutathione S-transferases M1 and T1 polymorphism with male infertility: a meta-analysis

Song

Zhao

Cai

et al. 2012

J Assist Reprod Genet

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Background Genes of different pathways regulate spermatogenesis, and the complexity of the spermatogenic process indicates that polymorphisms or mutations in these genes could cause male infertility. Published data on the association between the GSTM1 and GSTT1 polymorphism and male infertility risk are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. Methods A total of 11 studies regarding GSTM1 and 9 studies regarding GSTT1 between 1999 and 2012 were identified through researching MEDLINE, EMBASE and the Chinese Biomedical Database. It was performed to obtain summary estimated odd ratios and 95 % confidence intervals of GSTM1 and GSTT1 for male infertility, with attention to study quality and publication bias. Results Overall, a significant association was seen between GSTM1 (OR01.20, 95 % CI 01.02-1.40, P heterogeneity 0 0.000, P00.027) genotypes and male infertility. Significant associations were also observed in subgroups of Caucasian populations (OR01.65, 95 %CI01.16-2.34, P heterogeneity 0 0.006, P00.005), but were not observed in Asian populations (OR01.09, 95 % CI00.72-1.65, P heterogeneity 00.054, P00.697) when stratified by ethnicity. While there was no significant association was seen between GSTT1 (OR0 1.00, 95 % CI00.74-1.35, P heterogeneity 00.000, P00.980) null genotypes and male infertility. Simultaneously, significant associations were not observed in subgroups of Caucasian populations (OR 00.94, 95 %CI 00.44-2.00, P heterogeneity 00.000, P 00.867) and Asian populations (OR00.93, 95 % CI00.46-1.87, P heterogeneity 00.002, P0 0.838) when stratified by ethnicity. Conclusion Our results suggest the GSTM1 null genotype contributes to male infertility susceptibility, while GSTT1 gene polymorphisms are not associated with male infertility in our study.

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The association of glutathione-S-transferase gene polymorphisms (GSTM1, GSTT1, GSTP1) with idiopathic male infertility

Cited by 69 publications

References 38 publications

The Association of Seminal Plasma Antioxidant Levels and Sperm Chromatin Status with Genetic Variants ofGSTM1andGSTP1(Ile105Val and Ala114Val) in Infertile Men with Oligoasthenoteratozoospermia

The Association of Seminal Plasma Antioxidant Levels and Sperm Chromatin Status with Genetic Variants ofGSTM1andGSTP1(Ile105Val and Ala114Val) in Infertile Men with Oligoasthenoteratozoospermia

Glutathione S-transferase gene polymorphisms (GSTM1, GSTT1, GSTP1) and prostate cancer: a case-control study in Tehran, Iran

Association of the Glutathione S-transferases M1 and T1 polymorphism with male infertility: a meta-analysis

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