1996
DOI: 10.1111/j.1432-1033.1996.0805u.x
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The Association of Different Detergents with the Photosynthetic Reaction Center Protein of Rhodobacter sphaeroides R26 and the Effects on its Photochemistry

Abstract: Detergent-free reaction centers from Rhodobacter sphaeroides R26 were used to study the solubilization of reaction centers in various detergents and their effects on reaction center photochemistry. 500Z 100 n-octyl-P-D-glucopyranoside or 51 i 5 Triton X-100 molecules were associated with one reaction center. For N,N-alkylamine N-oxide detergents with chain lengths in the range from 8-13 carbon atoms, the number of detergent molecules associated with the reaction centers increased with decreasing chain length. … Show more

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Cited by 41 publications
(41 citation statements)
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“…S4. We note that many experimental parameters can cause shifts in the absorption peaks for the RC cofactors (45,46). In the case of the crystal used in Fig.…”
Section: Resultsmentioning
confidence: 99%
“…S4. We note that many experimental parameters can cause shifts in the absorption peaks for the RC cofactors (45,46). In the case of the crystal used in Fig.…”
Section: Resultsmentioning
confidence: 99%
“…This explains the slow exchange process of the quinone at its binding site. Conversely, the dimensions of Triton X-100 micelles [35] are larger than those formed by LDAO, thereby allowing a larger quinone pool size as well as higher ligand mobility.…”
Section: Discussionmentioning
confidence: 99%
“…Two site effects has been observed in bacterial reaction centers that cause significant modulation of the properties of the reaction center; these are the detergent effect on Rh. sphaeroides 74,75 and the effects of some large-scale mutations on Rb. capsulatus, 76 with spectral similarities 76 suggesting similar origins for these two effects.…”
Section: Discussionmentioning
confidence: 99%