The association between occurrence of plasmid-mediated quinolone resistance and ciprofloxacin resistance in Escherichia coli isolates of different origins

Yang, Tong; Zeng, Zhenling; Rao, Lili; Chen, Xiaojie; He, Dongjian; Lv, Luchao; Wang, Jing; Zeng, Li; Feng, Minsha; Liu, Jianhua

doi:10.1016/j.vetmic.2014.01.019

Cited by 40 publications

(23 citation statements)

References 29 publications

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“…In addition, four qnr -variants ( qnrA, qnrB, qnrD and qnrS ) were identified in the group of animal isolates, compared to only qnrB and qnrS among the human isolates. All identified qnr variants have already been identified in both groups, however no study is consistent with the results found here [41,42]. …”

Section: Discussionsupporting

confidence: 78%

Multiresistant extended-spectrum β-lactamase-producing Enterobacteriaceae from humans, companion animals and horses in central Hesse, Germany

et al. 2014

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BackgroundMultiresistant Gram-negative bacteria producing extended-spectrum β-lactamases (ESBLs) are an emerging problem in human and veterinary medicine. This study focused on comparative molecular characterization of β-lactamase and ESBL-producing Enterobacteriaceae isolates from central Hesse in Germany. Isolates originated from humans, companion animals (dogs and cats) and horses.ResultsIn this study 153 (83.6%) of the human isolates (n = 183) and 163 (91.6%) of the animal isolates (n = 178) were confirmed as ESBL producers by PCR and subsequent sequencing of the PCR amplicons. Predominant ESBL subtypes in human and animal samples were CTX-M-15 (49.3%) and CTX-M-1 (25.8%) respectively. Subtype blaCTX-M-2 was found almost exclusively in equine and was absent from human isolates. The carbapenemase OXA-48 was detected in 19 ertapenem-resistant companion animal isolates in this study. The Plasmid-encoded quinolone resistance (PMQR) gene aac(‘6)-Ib-cr was the most frequently detected antibiotic- resistance gene present in 27.9% of the human and 36.9% of the animal ciprofloxacin-resistant isolates. Combinations of two or up to six different resistance genes (penicillinases, ESBLs and PMQR) were detected in 70% of all isolates investigated. The most frequent species in this study was Escherichia coli (74%), followed by Klebsiella pneumoniae (17.5%), and Enterobacter cloacae (4.2%). Investigation of Escherichia coli phylogenetic groups revealed underrepresentation of group B2 within the animal isolates.ConclusionsIsolates from human, companion animals and horses shared several characteristics regarding presence of ESBL, PMQR and combination of different resistance genes. The results indicate active transmission and dissemination of multi-resistant Enterobacteriaceae among human and animal populations.

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Section: Discussionsupporting

confidence: 78%

Multiresistant extended-spectrum β-lactamase-producing Enterobacteriaceae from humans, companion animals and horses in central Hesse, Germany

et al. 2014

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“…Plasmid mediated quinolone resistances ( qnrS ) were found in 2(1.3%) of 151 E. coli chicken strains in Slovakia [22]. The frequency of PMQR genotypes in our study(37.1%) is lower than the report in China [19] and higher than that in Turkey,Nigeria and Slovakia. Typically, qnrB was considered to be the most prevalent PMQR gene in Enterobacteriaceae isolates in 2009 [5].…”

Section: Discussioncontrasting

confidence: 71%

“…A study by Tong et al showed that the prevalence of PMQR among isolates from chickens were 42.4% during 2004 to 2011 in China [19]. However, PMQR genes were detected in 5 (5.3%) of 94 E. coli chicken isolates in Turkey [20], and in 15 (15.6%)of 96 E. coli in 2006 in Nigeria [21].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Characteristics of Extended-Spectrum β-Lactamase and Plasmid-Mediated Fluoroquinolone Resistance Genes in Escherichia coli Isolated from Chickens in Anhui Province, China

Wang

Feng

et al. 2014

PLoS ONE

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The aim of this study was to characterize the prevalence of extended-spectrum β-lactamase (ESBL) genes and plasmid-mediated fluoroquinolone resistance (PMQR) determinants in 202 Escherichia coli isolates from chickens in Anhui Province, China, and to determine whether ESBL and PMQR genes co-localized in the isolates. Antimicrobial susceptibility for 12 antimicrobials was determined by broth microdilution. Polymerase chain reactions (PCRs), DNA sequencing, and pulsed field gel electrophoresis (PFGE) were employed to characterize the molecular basis for β-lactam and fluoroquinolone resistance. High rates of antimicrobial resistance were observed, 147 out of the 202 (72.8%) isolates were resistant to at least 6 antimicrobial agents and 28 (13.9%) of the isolates were resistant to at least 10 antimicrobials. The prevalence of bla CTX-M, bla TEM-1 and bla TEM-206 genes was 19.8%, 24.3% and 11.9%, respectively. Seventy-five out of the 202 (37.1%) isolates possessed a plasmid-mediated quinolone resistance determinant in the form of qnrS (n = 21); this determinant occurred occasionally in combination with aac(6′)-1b-cr (n = 65). Coexistence of ESBL and/or PMQR genes was identified in 31 of the isolates. Two E. coli isolates carried bla TEM-1, bla CTX-M and qnrS, while two others carried bla CTX-M, qnrS and aac(6′)-1b-cr. In addition, bla TEM-1, qnrS and aac(6′)-1b-cr were co-located in two other E. coli isolates. PFGE analysis showed that these isolates were not clonally related and were genetically diverse. To the best of our knowledge, this study is the first to describe detection of TEM-206-producing E. coli in farmed chickens, and the presence of bla TEM-206, qnrS and aac(6′)-1b-cr in one of the isolates.

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“…Wastewater treatment plants are considered as an important reservoir (hot-spots) of antibiotic-resistant bacteria (ARB), and antibiotic resistance genes (ARGs) [12][13][14] representing a major source of their dissemination in the environment [15,16]. The wastewater flowing into WWTPs create favorable conditions for the survival of ARB and transfer of ARGs, due to the high content of both microbial biomass and the nutrients [13].…”

Section: Introductionmentioning

confidence: 99%

Impact of type of wastewater treatment process on the antibiotic resistance of bacterial populations

et al. 2017

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Abstract. Antibiotic resistant bacteria reach the environment directly with faeces, and indirectly with sewage discharged from wastewater treatment plants (WWTPs). The aim of the study was to determine the level of removal of bacteria during wastewater treatment. Samples of untreated and treated sewage were collected from 13 WWTPs with different capacity, modification of treatment and type of inflowing wastewater. Microbiological characterization of the samples included determination of the total number of bacteria resistant (ARB) to β-lactams and tetracyclines as well as the number of Escherichia coli resistant to the same drugs. The counts of ARB and E. coli were determined on TSA and mFc media with/without antibiotic supplementation, respectively. The highest percent of reduction in number of ARB (at least 99.9%) and E. coli (above 99.3%) was obtained for WWTPs with A 2 O system. The lowest percentage reduction of ARB and E. coli was observed for WWTPs operating with SBR system. The lowest number of microorganisms resistant to analyzed antibiotics was observed for ARB and E. coli resistant to cefotaxime and doxycycline. The results indicate a large variation in the removal of antibiotic-resistant bacteria in WWTPs depending on modifications of treatment system and type of inflowing wastewater.

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The association between occurrence of plasmid-mediated quinolone resistance and ciprofloxacin resistance in Escherichia coli isolates of different origins

Cited by 40 publications

References 29 publications

Multiresistant extended-spectrum β-lactamase-producing Enterobacteriaceae from humans, companion animals and horses in central Hesse, Germany

Multiresistant extended-spectrum β-lactamase-producing Enterobacteriaceae from humans, companion animals and horses in central Hesse, Germany

Prevalence and Characteristics of Extended-Spectrum β-Lactamase and Plasmid-Mediated Fluoroquinolone Resistance Genes in Escherichia coli Isolated from Chickens in Anhui Province, China

Impact of type of wastewater treatment process on the antibiotic resistance of bacterial populations

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