The association between murine beta 2-microglobulin and HLA class I heavy chains results in serologically detectable conformational changes of both chains.

Ferrier, Pierre; Layet, Corine; Caillol, Danielle; Jordan, Bertrand; Lemonnier, F A

doi:10.4049/jimmunol.135.2.1281

Cited by 53 publications

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“…Cancer cells can evade T-cell recognition by downregulating or completely losing MHC class I molecules (34). The structural deficiency of MHC may change the expression of epitopes and increase the expression of β2-MG (35). Chen et al found that a higher level of β2-MG was significantly associated with greater invasion potential and tumor burden.…”

Section: Discussionmentioning

confidence: 99%

Clinicopathological characteristics and prognostic factors of primary pulmonary lymphoma

Tan

Xue

et al. 2021

J Thorac Dis

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Background: Primary pulmonary lymphoma (PPL) is a rare extranodal lymphoma originating from the lung, accounting for 0.5-1.0% of primary lung malignant tumors. Previous case reports or cohort studies included a limited sample size; therefore, the understanding of the disease remains inadequate, and clinical data regarding PPL are limited. Methods: Patients with PPL diagnosed histologically and radiologically between January 2000 and December 2019 at our center were retrospectively analyzed. Results: In total, 90 consecutive cases were included in this research. Forty-seven (52.2%) patients were female, and the median age was 54 years old. Non-Hodgkin's lymphoma (PPNHL) was the most common type of PPL (71/90, 78.9%), and mucosa-associated lymphoid tissue (MALT) lymphoma was the most common pathological subtype of PPNHL (56.3%) followed by diffuse large B-cell lymphoma (DLBCL) (32.4%). Thirty-nine (43.3%) patients underwent surgical treatment, and the others received chemotherapy alone or combined with radiotherapy. The estimated 5-year overall survival (OS) rates of MALT lymphoma and non-MALT lymphoma were 68.9% and 65.9%, respectively. Univariate analysis of PPL showed that clinicopathological features that significantly correlated with worse OS were age over 60 years (P=0.006<0.05), elevated LDH (P=0.029<0.05) and β2-MG (P=0.048<0.05) levels, clinical stage II2E and greater (P=0.015<0.05), and nonsurgical treatment (P=0.046<0.05). Age (P=0.013<0.05) was an independent prognostic factor for the 5-year OS of patients through multivariate analysis.Conclusions: Age over 60 years old, elevated LDH and β2-MG levels, clinical stage II2E disease or higher, and nonsurgical treatment were associated with poor prognosis in patients with PPL. Age can be used as a potential independent prognostic factor for PPL.

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Section: Discussionmentioning

confidence: 99%

Clinicopathological characteristics and prognostic factors of primary pulmonary lymphoma

Tan

Xue

et al. 2021

J Thorac Dis

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The prognostic significance of cytokine levels in newly diagnosed acute myeloid leukemia and high‐risk myelodysplastic syndromes

Tsimberidou

Estey

Wen

et al. 2008

Cancer

114

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BACKGROUND.Tumor necrosis factor (TNF)‐α and other cytokines are involved in the pathogenesis of acute myeloid leukemia (AML) and high‐risk myelodysplastic syndromes (MDS), but their prognostic significance in these diseases is unknown. In the current study, the authors assessed the association between serum levels of various cytokines and clinical outcomes in patients with untreated AML or high‐risk MDS.METHODS.Serum levels of TNF‐α, interleukin (IL)‐1 receptor antagonist, IL‐6, IL‐10, and endostatin were measured in patients with AML or high‐risk MDS who presented for treatment at The University of Texas M. D. Anderson Cancer Center from September 1994 through January 2001. Univariate and multivariate analyses were performed to test for correlations with clinical outcomes.RESULTS.Higher TNF‐α levels were found to correlate with poorer performance status; higher leukocyte counts; higher levels of β2‐microglobulin, creatinine, uric acid, and alkaline phosphatase; lower levels of creatinine clearance and albumin; baseline infection; and M4‐M5 AML subtypes. TNF‐α levels <10 pg/mL were associated with higher rates of complete remission (P = .003), survival (P = .0003), and event‐free survival (EFS) (P = .0009). However, on multivariate analyses, TNF‐α level ≥10 pg/mL was not found to be an independent factor predicting clinical outcomes, but became statistically significant when leukocyte count was excluded from the models. The other cytokines were not found to be predictive of clinical outcomes.CONCLUSIONS.High serum TNF‐α level is an adverse prognostic factor for survival and EFS in patients with untreated AML or high‐risk MDS. Cancer 2008. © 2008 American Cancer Society.

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Analysis of the expression of cloned HLA class I genes in mouse transfected L cells by quantitative flow cytometry

et al. 1985

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By using a calibrated cell sorter and monoclonal antibodies conjugated to fluorochromes, a quantitative analysis of the levels of expression of HLA class I molecules at the surface of cloned murine L cells transfected with purified A3, B7, or C W 3 genes was performed and compared with radioimmunoassay data. We selected clones of heterogenous levels of HLA class I expression, which were shown to remain constant over a period of 4 mo in absence of HAT selection and not to be correlated to the DNA copy number of the corresponding integrated gene.Key terms: DNA mediated gene transfer, monoclonal antibodies, HLA class I gene, flow cytometry.Recent technological developments in DNA-mediated gene transfer and flow cytometry (FCM) have made possible novel approaches to the analysis of the expression and regulation of gene products (10,311 such as those of the major histocompatibility complex (6,8,18,32). When total DNA is used for transfection, populations expressing very rare cell surface antigens can be isolated by repeated sortings (lO,ll), which gives valuable material to cloning of corresponding genes.When cloned genes are used, a qualitative analysis of their products expressed at the surface of transfected cells is generally performed (2,15,20,25). Beyond this qualitative analysis there is a need for quantitative data giving the number of molecules expressed, particularly for the study of the regulation of expression. Several methods using flow cytometry that allow such a quantitative analysis have been described (3,9,17,26,34).The aims of this paper are: 1) to define, with a Calibrated cell sorter and monoclonal antibodies (mAb) conjugated to fluorochrome (171, the quantitative levels of expression of HLA class I at the surface of selected cloned transfected L-A3, B7, CW3 cells; 2) to analyse the stability of this expression; and 3) to define whether a correlation existed between the levels of expression and the number of integrated HLA genes. MATERIALS AND METHODS Monoclonal Antibodies (mAb) and Their Conjugation to FluorochromeThe origin and characteristics of the mAb used are listed in Table 1. B9.12.1, B1.23.2, BlG6, 11-4.1, and 10.3.6 mAb were conjugated to fluorescein isothiocyanate (FITC) according to the method described in reference (17). Molar fluorochrome/protein (FP) ratios of FITC-conjugated mAb, determined after correction for quenching (17), were: B9.12.1,2.0; B1.232, 2.0; Bl.lG6, 1.2; 11.4.1,2.3; and 10.3.6,l.O. Transformation of Ltk-cellsCloned Ltk-murine fibroblasts (H-2kf, Iak-) were transfected by the calcium phosphate technique (35), either with cosmids 44 or 64 (HLA-A3) (19), cosmid 42 (HLA-CW3) (19), or cosmid 37 (HLA-B7) (12), all of which contained the herpes virus thymidine kinase (tk) gene. Control HLA-t k + cells were obtained by transfection with pAG0 DNA which contains the tk gene (20). TKt transfectants were selected in HAT medium (35). Transfected cells were cloned once or twice by limiting dilution (average 0.2 cell/well) and maintained in RPMI 1640 medium supplemented with 5% f...

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The association between murine beta 2-microglobulin and HLA class I heavy chains results in serologically detectable conformational changes of both chains.

Cited by 53 publications

References 0 publications

Clinicopathological characteristics and prognostic factors of primary pulmonary lymphoma

Clinicopathological characteristics and prognostic factors of primary pulmonary lymphoma

The prognostic significance of cytokine levels in newly diagnosed acute myeloid leukemia and high‐risk myelodysplastic syndromes

Analysis of the expression of cloned HLA class I genes in mouse transfected L cells by quantitative flow cytometry

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