The Association Between Idiopathic Hemolytic Uremic Syndrome and Infection by Verotoxin-Producing Escherichia coli

Karmali, Mohamed A.; Petric, Martin; Lim, Corazon; Fleming, Peter D.; Arbus, Gerald S.; Lior, H.

doi:10.1093/infdis/151.5.775

Cited by 1,251 publications

(453 citation statements)

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“…Hall and co-workers [36] reported dysentery in calves associated with E. coli (strain S102-9) found to be serogroup 0 5 was not seen in the course of the present study or in a previous study of VTEC( serotypes in Sri Lanka [31], although it has been found at low prevalence in cattle faecal specimens [25] and retail meat products from North America [39]. Of V'TEC 0 serogroups previously found in Sri Lanka [31], 0 86, 0 128, 0 84, 0 103, 0 111, and 0 153 have been associated with human diseases [7,. However, the H types of the strains isolated from Sri Lankan cattle were different from those associated with human disease in the temperate world [41].…”

Section: Discussioncontrasting

confidence: 46%

See 1 more Smart Citation

A longitudinal study of Vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka

Tokhi

Peiris

Scotland³

et al. 1993

Epidemiol. Infect.

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SUMIMIMARYTwo cohorts of 10 and 16 calves were followed at weekly or fortnightly intervals from 4-28 and 1-9 weeks respectively to determine whether natural infection by and an untvpable strain (0 ?.H21) were identified. All strains belonging to the samne serotype had identical profiles of reactivity with DNA probes to toxins VT1 or 2, LTI or II and a probe (CVD419) derived from a plasmid carried by enterohaemorrhagic Escherichia coli 0 157 . H7. Four of these serotypes were found in the faecal flora of the calves, taken as a group, throughout the 4-month study period.Sixty-percent of the strains hybridized with the probe for VT1, 4 % with the probe for V'T2, and 360% with both probes. Faecal V'TEC were significantly associated with overt diarrhoeal illness in animals < 10 weeks of age. but no characteristic profile of markers (serotype or hybridization pattern) in E. coli isolates was associated with diarrhoea. A serological response to VT1 wAas detected in some animnals, but faecal V-T1 V'TEC exeretion persisted in spite of seroconversion. VT1 seroconversion wNas not associated with diarrhoea. A serological response to NVT2 wras not detected even in those animals excreting VrT2 V'TEC in the faeces.

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Section: Discussioncontrasting

confidence: 46%

“…The V,Ts of E. coli are heterogeneous, two major groups being recognized, namely VT1 (SLTI) and VT2 (SLTII) [3]. Vero [2,4,5] and the haemolytic uraemic syndrome in man [6,7], and also of pig oedema disease [8]. More tentative associations have been made between VTEC and diarrhoea in calves [9], piglets [10] and cats [11].…”

Section: Introductionmentioning

confidence: 99%

A longitudinal study of Vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka

Tokhi

Peiris

Scotland³

et al. 1993

Epidemiol. Infect.

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“…Some workers have referred to these strains as enterohaemorrhagic E. coli (EHEC). VT+ strains have also been associated with cases of haemolytic-uraemic syndrome (HUS) in Canada (Karmali et al 1983(Karmali et al ,1985 and Britain (authors' unpublished results). Further studies show that VT closely resembles 'Shiga toxin', a cytotoxin produced by strains of Shigella dysenteriae 1 (O'Brien et al 1983), and it is well documented that infections with Sh.dysenteriae 1 may lead to HUS (Koster et al 1978).…”

Section: Pathogenesismentioning

confidence: 99%

Escherichia colidiarrhoea

Gross

Rowe

1985

J. Hyg.

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Ever since Escherich (1885) first isolated the organism now known asEscherichia colifrom the stools of infants, medical microbiologists have been faced with the problem of distinguishing between those strains capable of causing diarrhoea and those that are harmless gut commensals. Epidemiological investigations were greatly facilitated by the description by Kauffmann (1947) of a serotyping scheme forE. coli, and Taylor (1961) later reported that 17 0 serogroups ofE. colihad been implicated as possible causes of epidemic infantile enteritis. These infantile enteropathogenicE. coli(EPEC), having been discovered by epidemiological studies using serotyping, belonged by definition to a restricted range of serogroups. More recently it was shown that otherE. colistrains may produce enterotoxins, and these enterotoxigenicE. coli(ETEC) usually belong to particular serogroups which are different from those associated with EPEC.E. colistrains belonging to a third range of serogroups may cause an illness resembling shigella dysentery, and these may be regarded as entero-invasiveE. coli(EIEC).E. colistrains that cause diarrhoea may therefore be considered as three groups, as follows.

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“…Shigatoxin (Stx), produced by enterohemorrhagic Escherichia coli (EHEC) is associated with hemolytic uremic syndrome (HUS) (Karmali et al 1985). Stx consists of a single A subunit and a pentamer of B subunits (Jacewicz et al 1986;Lingwood et al 1998; S. Bouzari Á M. Oloomi (&) Á K. Azadmanesh Molecular Biology Unit, Pasteur Institute of Iran, Pasteur Ave., 13164 Tehran, Iran e-mail: manaoloomi@yahoo.com Endo et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Study on induction of apoptosis on HeLa and Vero cells by recombinant shiga toxin and its subunits

2009

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Verotoxin (VT) or shiga toxin (Stx) produced by enterohemorrhagic Escherichia coli (EHEC) and Shigella dysenteriae is AB5 holotoxin with potent protein synthesis inhibitor. VT can induce both apoptosis and necrosis depending on the cell type, it has been shown that VT-induced apoptosis and cytotoxicity are distinct processes, and the A subunit can be necessary for apoptosis. In other words, the precise role of each subunit in apoptosis signaling has yet to be established. In this study, induction of apoptosis has been examined by using both recombinant A and B subunits, and recombinant Stx (rStx) with different doses in HeLa and Vero cells. For this purpose, the polymyxin B extract of constructs expressing A, B and AB5 recombinant proteins was used. Therefore, amounts greater than normally reported were used to induce desire effects on cell lines. The apoptotic effect of A and B subunits appear at higher doses than that of rStx. The highest apoptotic effect was observed for rStx at low concentration, compared to A and B subunits. A or B subunits separately cannot induce the signaling pathway stimulated by holotoxin though A subunit, does induce laddering pattern similar to holotoxin. We concluded that both subunits are important in complete death signaling pathway. Since different concentration of A and B subunits and rStx was required in different assay, therefore, it could be emphasized that cell death or even apoptosis caused by either of the subunits or holotoxin depends on sensitivity or specificity of the assay and cell types used.

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The Association Between Idiopathic Hemolytic Uremic Syndrome and Infection by Verotoxin-Producing Escherichia coli

Cited by 1,251 publications

References 44 publications

A longitudinal study of Vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka

A longitudinal study of Vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka

Escherichia colidiarrhoea

Study on induction of apoptosis on HeLa and Vero cells by recombinant shiga toxin and its subunits

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