The association between gene polymorphism of certain cytokines (IL-6, IL-10) and hepatitis C virus infection locally

Abstract

Thirty blood samples were collected from people infected with HCV and collected 30 blood samples from healthy individuals as a control group. The samples were collected from the virus department at Diwaniyah Teaching Hospital, Women's Hospital and Educational Children and the Blood Bank in Diwaniyah Governorate, during the period from July 2018 until February 2019. Antibodies (Anti-HCV ) to HCV were detected in the infected serum using ELISA technique, and the diagnosis was confirmed Using the polymerase chain reaction test. Select gene polymorphism of interleukin -6 at site 174, which represents 3 genotypes GG, GC, CC in patients with HCV and control group. GG was significantly increased in patients with hepatitis C virus (63.3%) compared to the control group (30 %). The genotypes GC, CC were significantly decreased in patients with hepatitis C virus (16.7% and 20%) compared with the control group (43.3% and 26.7%), respectively. It also identified gene polymorphism of interleukin -10 at site 592, which represented 3 genotypes AA, AC, CC in patients with hepatitis C virus and control group. Genotype AC was significantly increased in patients with hepatitis C virus (53%) compared with the control group (30%). The genotypes CC, AA were significantly decreased in patients with hepatitis C virus (26.7% and 20%) compared with the control group (26.7% and 43.3%) respectively. Conclude from our current study that genotypes or alleles of IL-6 and IL-10 may play an essential role in increasing the risk of HCV in humans or play a vital role for prevention against.