The Association Between Breast Cancer and Blood-Based Methylation of S100P and HYAL2 in the Chinese Population

Yin, Qiming; Yang, Xiaoqin; Li, Lixi; Tian, Xu; Zhou, Wenjie; Gu, Wanjian; Ma, Fei; Yang, Rongxi

doi:10.3389/fgene.2020.00977

Cited by 9 publications

(4 citation statements)

References 49 publications

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“…The Genomic DNA Extraction Kit (Rebece, Nanjing, China) was used to extract human peripheral blood cells' genomic DNA as described previously [37]. Then the extracted DNA was further bisulfate-converted by EZ-96 DNA Methylation Gold Kit (Zymo Research, Orange County, USA) according to the manufacturer's protocol.…”

Section: Dna Processing and Bisulfite Conversionmentioning

confidence: 99%

HTRA1 methylation in peripheral blood as a potential marker for the preclinical detection of stroke: a case–control study and a prospective nested case–control study

Liu

Yin

et al. 2022

Clin Epigenet

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Background Stroke is the leading cause of mortality in China. DNA methylation has essential roles in multiple diseases, but its association with stroke was barely studied. We hereby explored the association between blood-based HTRA serine protease 1 (HTRA1) methylation and the risk of stroke. Results The association was discovered in a hospital-based case–control study (cases/controls = 190:190) and further validated in a prospective nested case–control study including 139 cases who developed stroke within 2 years after recruitment and 144 matched stroke-free controls. We observed stroke-related altered HTRA1 methylation and expression in both case–control study and prospective study. This blood-based HTRA1 methylation was associated with stroke independently from the known risk factors and mostly affected the older population. The prospective results further showed that the altered HTRA1 methylation was detectable 2 years before the clinical determination of stroke and became more robust with increased discriminatory power for stroke along with time when combined with other known stroke-related variables [onset time ≤ 1 year: area under the curve (AUC) = 0.76]. Conclusions In our study, altered HTRA1 methylation was associated with stroke at clinical and preclinical stages and thus may provide a potential biomarker in the blood for the risk evaluation and preclinical detection of stroke.

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Section: Dna Processing and Bisulfite Conversionmentioning

confidence: 99%

HTRA1 methylation in peripheral blood as a potential marker for the preclinical detection of stroke: a case–control study and a prospective nested case–control study

Liu

Yin

et al. 2022

Clin Epigenet

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“…AHRR methylation has been associated with CHD in Caucasians, but could not be validated in the Chinese population. Other studies have shown that ethnic genetic background or lifestyle could play a role in epigenetic modifications [46][47][48]. Thus, DNA methylation patterns warrant validation when the different ethnic population is considered.…”

Section: Discussionmentioning

confidence: 99%

The association between DNA methylation of 6p21.33 and AHRR in blood and coronary heart disease in Chinese population

Zhu

Yang

et al. 2022

BMC Cardiovasc Disord

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Background Early detection could significantly improve the prognosis of coronary heart disease (CHD). In-invitro diagnostic technique may provide a solution when sufficient biomarkers could be identified. Pertinent associations between blood-based aberrant DNA methylation and smoking, the pathogenesis of atherosclerosis, and CHD have been robustly demonstrated and replicated, but that studies in Chinese populations are rare. The blood-based methylation of aryl-hydrocarbon receptor repressor (AHRR) cg05575921 and 6p21.33 cg06126421 has been associated with cardiovascular mortality in Caucasians. Here, we aim to investigate whether the AHRR and 6p21.33 methylation in the blood is associated with CHD in the Chinese population. Methods In this case–control study, 180 CHD patients recruited at their first registration in our study center, and 184 controls randomly selected from the people who participated in the annual health examination were enrolled. Methylation intensities of 19 CpG sites, including AHRR cg05575921, 6p21.33 cg06126421, and their flanking CpG sites, were quantified by mass spectrometry. The association between methylation intensities and CHD was estimated by logistic regression analyses adjusted for covariant. Results Compared to the controls, lower methylation of 6p21.33_CpG_4.5/cg06126421 was independently associated with increased odds of being a CHD patient (OR per − 10% methylation = 1.42 after adjustment for age, gender, and batch effect; p = 0.032 by multiple testing corrections). No association between blood-based AHRR methylation and CHD was found. Conclusions 6p21.33 methylation exhibits a significant association with CHD. The combination of 6p21.33 methylation and conventional risk factors might be an intermediate step towards the early detection of CHD.

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“…Agena MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometry (Agena Bioscience, San Diego, CA, USA) described by Yang et al ( 24 ) and Yin et al ( 25 ) was used to determine the level of DNA methylation quantitatively. Briefly, the bisulfite-converted DNA was amplified by bisulfite-specific primers ( Supplementary Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

The association between ACTB methylation in peripheral blood and coronary heart disease in a case-control study

Jin

Zhu

Zhao

et al. 2022

Front. Cardiovasc. Med.

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BackgroundCoronary heart disease (CHD) brings a heavy burden to society worldwide. Novel and minimally invasive biomarkers for the risk evaluation of CHD are urgently needed. Previous study has revealed that blood-based hypomethylation of β-actin (ACTB) was associated with increased risk of stroke, but not reported in CHD yet.ObjectivesWe aimed to explore the association between blood-based ACTB methylation and the risk of CHD in a case-control study in the Chinese population.MethodsThe methylation level of ACTB was quantitatively determined by mass spectrometry in 281 CHD patients and 272 controls. The association between ACTB methylation and CHD risk was estimated by logistic regression analyses adjusted for possible confounding effects.ResultsWe found a significant association between hypermethylation of ACTB in peripheral blood and increased risk of CHD (odds ratios (ORs) per +10% methylation: 1.19–1.45, p < 0.013 for nine out of thirteen CpG sites), especially in male subjects and heart failure (HF) patients (ORs per +10% methylation: 1.20–1.43, 1.38–1.46; p < 0.030, 1.52 × 10−4, respectively). Hypermethylation of ACTB_CpG_2.3, ACTB_CpG_7.8, and ACTB_CpG_9.10 was observed in the CHD patients with minor to medium cardiac function impairment (NYHA I&II CHD cases) (ORs per +10% methylation: 1.38–1.44; p < 0.001). The combination of ACTB_CpG_2.3, ACTB_CpG_7.8, and ACTB_CpG_9.10 methylation levels could efficiently discriminate CHD cases, male CHD patients, HF and NYHA I&II CHD patients from controls (area under curve (AUC) = 0.75, 0.74, 0.73, and 0.77, respectively).ConclusionsOur study reveals a strong association between blood-based ACTB hypermethylation and CHD risk. The combination of ACTB methylation and conventional risk factors might provide a novel strategy to improve risk assessment of CHD.

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The Association Between Breast Cancer and Blood-Based Methylation of S100P and HYAL2 in the Chinese Population

Cited by 9 publications

References 49 publications

HTRA1 methylation in peripheral blood as a potential marker for the preclinical detection of stroke: a case–control study and a prospective nested case–control study

HTRA1 methylation in peripheral blood as a potential marker for the preclinical detection of stroke: a case–control study and a prospective nested case–control study

The association between DNA methylation of 6p21.33 and AHRR in blood and coronary heart disease in Chinese population

The association between ACTB methylation in peripheral blood and coronary heart disease in a case-control study

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