Tenderness is greatly impacted the organoleptic and quality characteristics of meat and its products (Bojorges et al., 2020;Brito Lopes et al., 2020;Ma & Kim, 2020). Inconsistency and nonuniformly texturized meat products are the major problems encountered by the meat industry that affecting tenderness and also customer satisfaction (Stadnik & Dolatowski, 2011;Uyarcan & Kayaardi, 2019;Zhou et al., 2020). Hence, over the past few decades, the industrial sections concentrated on discovering safe and innovative ingredients and methods to solve this issue. In aged animals, firmness is accompanied by the development of perimysium, endomysium thickness, and the generation of cross-links between collagen molecules.