The assessment of apoptosis, toxicity effects and anti-leishmanial study of Chitosan/CdO core-shell nanoparticles, eco-friendly synthesis and evaluation

Bahraminegad, Sina; Pardakhty, Abbas; Sharifi, Iraj; Ranjbar, Mehdi

doi:10.1016/j.arabjc.2021.103085

Cited by 11 publications

(6 citation statements)

References 35 publications

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“…Subsequently, there is no significant change in mass. In the CoFe 2 O 4 @CTS samples, the polymer degradation temperature was observed as a well-defined drop of 8.9% (by mass) at 182.5 °C. , The value is like that obtained by Morawski et al, who synthesized nanoparticles of MnFe 2 O 4 @CTS using a similar methodology. The authors reported that 7.4% was the amount of CTS incorporated into the surface of the nanoparticles in the core, providing core–shell nanoparticles with high magnetic character confirmed by VSM measurements. , …”

Section: Resultssupporting

confidence: 80%

Core–Shell Chitosan Cobalt Ferrite Nanoparticles for Ultrasensitive Simultaneous Voltammetric Determination of Pb(II) and Cd(II)

Neto,

Costa,

Morawski

et al. 2024

ACS Appl. Nano Mater.

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In water samples from industrial regions, the presence of highly toxic and nonbiodegradable heavy metal ions (HMI) poses a significant threat to environmental quality. While analytical methods based on mercury electrodes are widely recognized for their high performance in the sensitive and selective quantification of various metal contaminants, the development of environmentally friendly strategies for the voltammetric determination of HMI remains a formidable challenge. In this study, we propose a material utilizing small-size chitosan (CTS) cobalt ferrite core–shell nanoparticles (CoFe2O4@CTS) as an electrode modifier for the simultaneous voltammetric determination of Pb(II) and Cd(II). The magnetic nanoparticles were synthesized using the solvothermal method and characterized using various techniques to explore their crystalline structure, magnetic properties, and chemical composition. The resulting electrochemical sensor, CoFe2O4@CTS/GCE, was generated through a one-step modification of a glassy carbon electrode (GCE). Utilizing differential pulse adsorptive stripping voltammetry (DPAdSV), our proposed sensor exhibited high sensitivity, with low limits of detection (LOD) values of 0.04 and 0.31 μg L–1 (E d = −1.0 V; t d = 90 s) for lead and cadmium, respectively. The exceptional sensitivity observed is indicative of interactions between HMI and CTS amine groups, leading to the effective preconcentration of the analytes. Furthermore, our sensor demonstrated excellent selectivity with an interference response below 8.93% for Pb(II) and 20% for Cd(II). Accuracy was evaluated using SRM 1643e (NIST). The application of green analytical chemistry (GAC) metrics resulted in a score of 0.91, highlighting the environmentally conscious aspects of our methodology.

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Section: Resultssupporting

confidence: 80%

Core–Shell Chitosan Cobalt Ferrite Nanoparticles for Ultrasensitive Simultaneous Voltammetric Determination of Pb(II) and Cd(II)

Neto,

Costa,

Morawski

et al. 2024

ACS Appl. Nano Mater.

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“…Then they were washed twice with the cold PBS solution and 1 mL of binding buffer was added to them. Finally, 100 µL of the whole mixture was removed and 5 µL of annexin V-IFTC and 5 µL of 7aminoactinomycin D (AAD7) were added to it and the mixture was subsequently evaluated by a flow cytometer [6,40].…”

Section: Evaluation Of Drug Toxicity To Macrophagesmentioning

confidence: 99%

“…The apoptotic, necrotic, and viable cells percentage for each sample was determined by analyzing the flow cytometry diagrams using FlowJo software. For this purpose, 6 promastigotes/ml of L. major were cultured in RPMI medium containing 10% (v/v) fetal bovine serum (FBS-Serum, Germany) at 25 °C with 5% CO2. According to the kit instructions, the parasites were stained by annexin V-IFTC and 7AAD and analyzed using a Becton Dickinson flow cytometer.…”

Section: Flow Cytometry Apoptosis Assaymentioning

confidence: 99%

“…According to the World Health Organization (WHO), leishmaniasis as a major public health problem is one of the seven most important tropical diseases due to its wide spectrum of clinical manifestations [3], [4,5]. Leishmaniasis is a sophisticated disease for both humans and animals [6,7]. Cutaneous manifestation include localised cutaneous leishmaniasis [LCL] (ulcerative skin lesions) and diffuse cutaneous leishmaniasis [DCL] (multiple non-ulcerative nodules) [5,8,9].…”

Section: Introductionmentioning

confidence: 99%

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Therapeutic Effects of The As-Synthesized Polylactic Acid/Chitosan Nanofibers Decorated With Amphotricin B For In Vitro Treatment of Leishmaniasis

Bahraminegad

Pardakhty

Sharifi

et al. 2021

Preprint

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Leishmaniasis as a third most important vector-borne disease caused by different species of a Leishmania flagellum protozoan. Leishmaniasis as a one of the biggest concerns of the World Health Organization due to the severe side effects, the emergence of resistance, secondary bacterial infections, reports as epidemics especially in immunocompromised individuals. The main purpose of this study was synthesis and characterization of the polylactic acid/chitosan nanofibers decorated with amphotricin B as a new delivery system for in vitro treatment of Leishmania wounds. The prepared nanofibers were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), Dynamic light scattering (DLS), X-ray diffraction (XRD) and in vitro drug release test. The anti leishmaniasis effect of nanofibers decorated with amphotricin B against Leishmania major promastigotes and its cytotoxicity on macrophages were determined respectively with flow cytometry test. The in-vitro drug release assay indicates 84% of the amphotricin B loaded in nanofibers after 400 minutes. The average concentration of the amphotricin B loaded in polylactic acid/chitosan nanofibers and conventional form of amphotricin B that prevented the growth of 50% of the promastigotes of L.major was 1.29 and 4.34 µg/ml, respectively.

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“…Based on the World Health Organization (WHO), about 12 million people are infected with this disease, and 1 million people are added to this number annually. Nearly 70 percent have been reported in 10 countries: Afghanistan, Iran, Syria, Sudan, Brazil, Peru, Costa Rica, Syria, and Saudi Arabia ( World Health Organization, 2020a ; Bahraminegad et al, 2021 ). Many determinants, including the human immunodeficiency virus (HIV) outbreak, a rise in international travel, the absence of efficacious vaccines, problems in controlling vectors, global conflicts, and the expansion of resistance to drugs, could upsurge the incidence of leishmaniasis ( Sereno et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Amphotericin B and AmBisome: In Silico and In Vivo Evaluation Employing the Chick Embryo Model

et al. 2022

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Leishmaniasis has been identified as a significant disease in tropical and subtropical regions of the world, with Iran being one of the disease-endemic areas. Various treatments have been applied for this disease, and amphotericin B (Amp B) is the second line of treatment. Side effects of this drug have been reported in various organs. The present study investigated the effects of different types of Amp B on fetal organs using in silico and in vivo assays (chicken embryos). In vivo analysis was done by checking pathological changes, angiogenesis, and apoptosis alterations on eggs treated by Amp B and AmBisome. In silico approach was employed to predict the affinity of Amp B and AmBisome to the vascular endothelial growth factor A (VEGF-A), its receptor (KDR1), apoptotic-regulator proteins (Bcl-2-associated X protein (Bax), B-cell lymphoma (Bcl-2), and Caspase-8. The ADME-toxicity prediction reveals that AmBisome possesses a superior pharmacological effect to Amp B. The best result of all the dockings in the Molegro Virtual Docker (MVD) was obtained between Bax, Bcl-2, Caspase-8, KDR1, and VEGF-A targets. Due to the lower Egap (HOMO–LUMO) of AmBisome, the chemical reactivity of AmBisome was higher than that of Amp B. In vivo analysis showed that embryos that received Amp B exhibited less vascular density than AmBisome. Amp B alone significantly increased the expression of apoptosis and decreased angiogenesis genes compared to AmBisome. The histopathology analysis of the treated embryos showed a reduction in the blood vessel collapse and an increase in degenerative and apoptotic–necrotic changes in the embryonic tissues. Overall, the results suggest the potential benefits of AmBisome over Amp B, which might be a better treatment strategy to treat leishmaniasis during pregnancy.

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The assessment of apoptosis, toxicity effects and anti-leishmanial study of Chitosan/CdO core-shell nanoparticles, eco-friendly synthesis and evaluation

Cited by 11 publications

References 35 publications

Core–Shell Chitosan Cobalt Ferrite Nanoparticles for Ultrasensitive Simultaneous Voltammetric Determination of Pb(II) and Cd(II)

Core–Shell Chitosan Cobalt Ferrite Nanoparticles for Ultrasensitive Simultaneous Voltammetric Determination of Pb(II) and Cd(II)

Therapeutic Effects of The As-Synthesized Polylactic Acid/Chitosan Nanofibers Decorated With Amphotricin B For In Vitro Treatment of Leishmaniasis

Cytotoxicity of Amphotericin B and AmBisome: In Silico and In Vivo Evaluation Employing the Chick Embryo Model

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