The assembly of photosynthetic proteins

Choquet, Yves; Wollman, Françis-André

doi:10.1016/b978-0-12-821430-5.00007-9

Cited by 6 publications

(6 citation statements)

References 215 publications

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“…In Chlamydomonas , CES has been observed in the assembly of cytochrome b 6 f, ATP synthase, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), and PSI. It is also involved in the synthesis of Rubisco in plants and Cox1 in yeast mitochondria (see [ 79 ]).…”

Section: Discussionmentioning

confidence: 99%

Assembly and Repair of Photosystem II in Chlamydomonas reinhardtii

Mehra,

Wang,

Russell

et al. 2024

Plants

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Oxygenic photosynthetic organisms use Photosystem II (PSII) to oxidize water and reduce plastoquinone. Here, we review the mechanisms by which PSII is assembled and turned over in the model green alga Chlamydomonas reinhardtii. This species has been used to make key discoveries in PSII research due to its metabolic flexibility and amenability to genetic approaches. PSII subunits originate from both nuclear and chloroplastic gene products in Chlamydomonas. Nuclear-encoded PSII subunits are transported into the chloroplast and chloroplast-encoded PSII subunits are translated by a coordinated mechanism. Active PSII dimers are built from discrete reaction center complexes in a process facilitated by assembly factors. The phosphorylation of core subunits affects supercomplex formation and localization within the thylakoid network. Proteolysis primarily targets the D1 subunit, which when replaced, allows PSII to be reactivated and completes a repair cycle. While PSII has been extensively studied using Chlamydomonas as a model species, important questions remain about its assembly and repair which are presented here.

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Section: Discussionmentioning

confidence: 99%

Assembly and Repair of Photosystem II in Chlamydomonas reinhardtii

Mehra,

Wang,

Russell

et al. 2024

Plants

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“…Indeed, Synechocystis Psb28-1 was proposed to play a role in regulating chlorophyll incorporation into CP47 and PSI (Beckova et al, 2017). Alternatively, PSII assembly intermediates specifically accumulating in the psb28 mutant might act as regulators of other processes resembling CES (Choquet and Wollman, 2023). It is also possible that PSII intermediates in the psb28 mutant specifically bind assembly factors, chaperones or proteases, which are then not sufficiently available for other chloroplast processes.…”

Section: Discussionmentioning

confidence: 99%

Complexome profiling of the Chlamydomonaspsb28mutant reveals THYLAKOID ENRICHED FRACTION 5 as an early photosystem II assembly factor

Lang,

König,

Venn

et al. 2024

Preprint

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Several auxiliary factors are required for the assembly of photosystem (PS) II, one of which is Psb28. While the absence of Psb28 in cyanobacteria has little effect on PSII assembly, we show here that the Chlamydomonaspsb28-null mutant is severely impaired in PSII assembly, showing drastically reduced PSII supercomplexes, dimers and monomers, while overaccumulating RCII, CP43modand D1mod. The mutant had less PSI and more Cytb6fand showed fewer thylakoid stacks and distorted chloroplast morphology. Complexome profiling of thepsb28mutant revealed that TEF5, the homolog of Arabidopsis PSB33/LIL8, co-migrated particularly with RCII. TEF5 also interacted with PSI. A Chlamydomonastef5null mutant is also severely impaired in PSII assembly and overaccumulates RCII and CP43mod. RC47 was not detectable in the light-growntef5mutant. Our data suggest a possible role for TEF5 in facilitating the assembly of CP47modinto RCII. Both thepsb28andtef5mutants exhibited decreased synthesis of CP47 and PsbH, suggesting negative feedback regulation possibly exerted by the accumulation of RCII and/or CP43modin both mutants. The strong effects of missing auxiliary factors on PSII assembly in Chlamydomonas suggest a more effective protein quality control system in this alga than in land plants and cyanobacteria.One-sentence summaryThe Chlamydomonas psb28 mutant is severely impaired in PSII assembly which via complexome profiling allowed identifying TEF5 as a novel PSII assembly factor that likely facilitates CP47 assembly.The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (https://academic.oup.com/plcell/pages/General-Instructions) is: Michael Schroda (m.schroda@rptu.de).

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“…Rubisco catalyzes the first step in two opposing chemical pathways: photorespiration (using O2 as a substrate) and photosynthetic carbon fixation (using CO2 as a substrate) ( Andrews and Lorimer, 1985 ; Erb and Zarzycki, 2018 ). The photosynthetic uptake of CO 2 results in the production of functioning sugars ( Gutteridge and Gatenby, 1995 ; Choquet and Wollman, 2023 ), which are responsible for plant development and yield ( Saschenbrecker et al., 2007 ). The Glyma.18g297900 gene that was identified in the flanking region of ss715632502, a QTN for seed weight per plant, is homologous to the Arabidopsis gene AT1G67140.3 ( SWEETIE ) and encodes HEAT repeat-containing protein.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association study of soybean (Glycine max [L.] Merr.) germplasm for dissecting the quantitative trait nucleotides and candidate genes underlying yield-related traits

et al. 2023

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Soybean (Glycine max [L.] Merr.) is one of the most significant crops in the world in terms of oil and protein. Owing to the rising demand for soybean products, there is an increasing need for improved varieties for more productive farming. However, complex correlation patterns among quantitative traits along with genetic interactions pose a challenge for soybean breeding. Association studies play an important role in the identification of accession with useful alleles by locating genomic sites associated with the phenotype in germplasm collections. In the present study, a genome-wide association study was carried out for seven agronomic and yield-related traits. A field experiment was conducted in 2015/2016 at two locations that include 155 diverse soybean germplasm. These germplasms were genotyped using SoySNP50K Illumina Infinium Bead-Chip. A total of 51 markers were identified for node number, plant height, pods per plant, seeds per plant, seed weight per plant, hundred-grain weight, and total yield using a multi-locus linear mixed model (MLMM) in FarmCPU. Among these significant SNPs, 18 were putative novel QTNs, while 33 co-localized with previously reported QTLs. A total of 2,356 genes were found in 250 kb upstream and downstream of significant SNPs, of which 17 genes were functional and the rest were hypothetical proteins. These 17 candidate genes were located in the region of 14 QTNs, of which ss715580365, ss715608427, ss715632502, and ss715620131 are novel QTNs for PH, PPP, SDPP, and TY respectively. Four candidate genes, Glyma.01g199200, Glyma.10g065700, Glyma.18g297900, and Glyma.14g009900, were identified in the vicinity of these novel QTNs, which encode lsd one like 1, Ergosterol biosynthesis ERG4/ERG24 family, HEAT repeat-containing protein, and RbcX2, respectively. Although further experimental validation of these candidate genes is required, several appear to be involved in growth and developmental processes related to the respective agronomic traits when compared with their homologs in Arabidopsis thaliana. This study supports the usefulness of association studies and provides valuable data for functional markers and investigating candidate genes within a diverse germplasm collection in future breeding programs.

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The assembly of photosynthetic proteins

Cited by 6 publications

References 215 publications

Assembly and Repair of Photosystem II in Chlamydomonas reinhardtii

Assembly and Repair of Photosystem II in Chlamydomonas reinhardtii

Complexome profiling of the Chlamydomonaspsb28mutant reveals THYLAKOID ENRICHED FRACTION 5 as an early photosystem II assembly factor

Genome-wide association study of soybean (Glycine max [L.] Merr.) germplasm for dissecting the quantitative trait nucleotides and candidate genes underlying yield-related traits

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